Levels and molecular size distribution of serum laminin in adult type I diabetic patients with and without microangiopathy

Werle, E; Diehl, E.; Hasslacher, Christoph

doi:10.1016/s0026-0495(98)90194-9

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…All tested surfaces induced higher CaP precipitation when incubated in SBF containing laminin with a concentration equivalent to the human blood plasma as compared to blasted surfaces solely incubated in SBF [36]. A possible mechanism for laminin promoting CaP precipitation may be its function as a nucleation center.…”

Section: Discussionmentioning

confidence: 99%

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In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

Bougas

Stenport²,

Currie³

et al. 2011

JOMR

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ObjectivesThe aim of the present study was to evaluate calcium phosphate precipitation and the amount of precipitated protein on three potentially bioactive surfaces when adding laminin in simulated body fluid.Material and MethodsBlasted titanium discs were prepared by three different techniques claimed to provide bioactivity: alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA). A blasted surface incubated in laminin-containing simulated body fuid served as a positive control (B) while a blasted surface incubated in non laminin-containing simulated body fuid served as a negative control (B-). The immersion time was 1 hour, 24 hours, 72 hours and 1 week. Surface topography was investigated by interferometry and morphology by Scanning Electron Microscopy (SEM). Analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX) and the adsorbed laminin was quantified by iodine (125I) labeling.ResultsSEM demonstrated that all specimens except for the negative control were totally covered with calcium phosphate (CaP) after 1 week. EDX revealed that B- demonstrated lower sum of Ca and P levels compared to the other groups after 1 week. Iodine labeling demonstrated that laminin precipitated in a similar manner on the possibly bioactive surfaces as on the positive control surface.ConclusionsOur results indicate that laminin precipitates equally on all tested titanium surfaces and may function as a nucleation center thus locally elevating the calcium concentration. Nevertheless further studies are required to clarify the role of laminin in the interaction of biomaterials with the host bone tissue.

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Section: Discussionmentioning

confidence: 99%

“…HEPES was dissolved in 100 ml distilled water before being added to the solution and the final pH was adjusted to 7.4 at 37 °C. Laminin marked with 125 I was added to adjust to a final concentration of 300 ng/ml, which corresponds to the concentration of laminin included in the human blood plasma [36]. …”

Section: Methodsmentioning

confidence: 99%

In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

Bougas

Stenport²,

Currie³

et al. 2011

JOMR

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“…Because the serum levels of basement membrane (BM) constituents could reflect these metabolic changes, their usefulness as markers of diabetic microangiopathy has been extensively investigated. In this regard, serum levels of laminin, a major specific non-collagenous glycoprotein of BM, have been proposed as an index of diabetic microangiopathy,2 and high concentrations have been reported in patients with diabetes with proliferative diabetic retinopathy 23 Notably, laminin contributes to the net increase in BMs that occurs during diabetic retinopathy (DR) and is one of the main constituents of the periodic acid Schiff positive material deposited in BMs of retinal vessels of patients with DR 4…”

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confidence: 99%

Effect of panretinal photocoagulation on serum levels of laminin in patients with diabetes: a prospective study

Masmiquel¹,

Burgos²,

Mateo³

et al. 1999

British Journal of Ophthalmology

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Background/aim-Laminin, a major specific non-collagenous glycoprotein of basement membrane, has been proposed as an index of diabetic retinopathy and high serum concentrations have been reported in patients with proliferative diabetic retinopathy. On the other hand, panretinal photocoagulation (PRP) prevents the progression of severe diabetic retinopathy and reverses preretinal neovascularisation. The aim of the study was to investigate the eVect of PRP on serum levels of laminin in patients with diabetes. Methods-20 patients with diabetes undergoing PRP and 15 patients with mild or moderate non-proliferative diabetic retinopathy in whom a PRP was not performed were included in the study. Serum laminin-P1 (Lam-P1), the largest pepsin resistant fragment of laminin, was determined by radioimmunoassay in each patient before starting PRP and 3 months after it was accomplished. Similarly, a baseline and a 4 month sample were analysed in the non-photocoagulated controls. Results-Serum Conclusion-PRPdecreases serum Lam-P1 levels in patients with severe diabetic retinopathy. Thus, the studies addressed to evaluate the usefulness of Lam-P1 as a marker of diabetic retinopathy should consider previous PRP as an influencing factor. Finally, our results suggest that retinal source of Lam-P1 strongly contributes to serum Lam-P1 in patients with severe diabetic retinopathy. (Br J Ophthalmol 1999;83:1056-1059

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Laminin inhibition of ?-amyloid protein (A?) fibrillogenesis and identification of an A? binding site localized to the globular domain repeats on the laminin a chain

et al. 2000

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β‐Amyloid protein (Aβ) is a major component of neuritic plaques and cerebrovascular amyloid deposits in the brains of patients with Alzheimer's disease (AD). Inhibitors of Aβ fibrillogenesis are currently sought as potential future therapeutics for AD and related disorders. In the present study, the basement membrane protein laminin was found to bind Aβ 1–40 with a single dissociation constant, Kd = 2.7 × 10–9 M, and serve as a potent inhibitor of Aβ fibril formation. 25 μM of Aβ 1–40 was incubated at 37°C for 1 week in the presence of 100 nM of laminin or other basement membrane components, including perlecan, type IV collagen, and fibronectin to determine their effects on Aβ fibril formation as evaluated by thioflavin T fluorometry. Of all the basement membrane components tested, laminin demonstrated the greatest inhibitory effect on Aβ‐amyloid fibril formation, causing a ninefold inhibition at 1 and 3 days and a 21‐fold inhibition at 1 week. The inhibitory effects of laminin on Aβ fibrillogenesis occurred in a dose‐dependent manner and were still effective at lower concentrations. The inhibitory effects of laminin on Aβ 1–40 fibril formation was confirmed by negative stain electron microscopy, whereby laminin caused an almost complete inhibition of Aβ fibril formation and assembly by 3 days, resulting in the appearance of primarily amorphous nonfibrillar material. Laminin also caused partial disassembly of preformed Aβ‐amyloid fibrils following 4 days of coincubation. Laminin was not effective as an inhibitor of islet amyloid polypeptide fibril formation, suggesting that laminin's amyloid inhibitory effects were Aβ‐specific. To identify a potential Aβ‐binding site(s) on laminin, laminin was first digested with V8, trypsin, or elastase. An Aβ‐binding elastase digestion product of ∼120–130 kDa was found. In addition, a ∼55 kDa fragment derived from V8 and elastase‐digested laminin interacted with biotinylated Aβ 1–40. Amino acid sequencing of the ∼55 kDa fragment identified a conformationally dependent Aβ‐binding site within laminin localized to the globular repeats on the laminin A chain. These studies demonstrate that laminin not only binds Aβ with relatively high affinity but is a potent inhibitor of Aβ‐amyloid fibril formation. In addition, further identification of an Aβ‐binding domain within the globular repeats on the laminin A chain may lead to the design of new therapeutics for the inhibition of Aβ fibrillogenesis. J. Neurosci. Res. 62:451–462, 2000. © 2000 Wiley‐Liss, Inc.

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Levels and molecular size distribution of serum laminin in adult type I diabetic patients with and without microangiopathy

Cited by 6 publications

References 27 publications

In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

In vitro Evaluation of Calcium Phosphate Precipitation on Possibly Bioactive Titanium Surfaces in the Presence of Laminin

Effect of panretinal photocoagulation on serum levels of laminin in patients with diabetes: a prospective study

Laminin inhibition of ?-amyloid protein (A?) fibrillogenesis and identification of an A? binding site localized to the globular domain repeats on the laminin a chain

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