Levels of complement components iC3b, C3c, C4, and SC5b-9 in peritoneal fluid and serum of infertile women with endometriosis

Kabut, Jacek; Kondera-Anasz, Zdzisława; Sikora, Justyna; Mielczarek‐Palacz, Aleksandra

doi:10.1016/j.fertnstert.2006.12.061

Cited by 34 publications

(20 citation statements)

References 42 publications

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“…As anticipated, the macrophage secretion signature collectively ranked very high within this ordering, along with canonical macrophage surface markers such as CD14, CD68, and HLA-A/B/C. Moreover, numerous gene products previously associated with endometriosis in independent studies were observed within the top ten percent of genes in the co-expression profile, including IL-23 (26), soluble TNF receptors I & II (27), MIP-3β/CCL19 (28), ENA-78/CXCL5 (29), MMP-9 (30), CCR1 (31), C3 (32), NFκB (33), and Cox-1/2 (34). To verify that these inferences were not unique to the expression structure present in IRIS, we examined a second co-expression profile derived from the Human Immune Cell Transcriptome (HICT) compendia (35), which demonstrated broad correspondence with IRIS profile rankings ( P < 10 −9 , Spearman correlation) and comparable enrichment of the independently validated inflammatory markers noted above among the top ten percent of proximal co-expression partners.…”

Section: Resultsmentioning

confidence: 95%

Molecular Network Analysis of Endometriosis Reveals a Role for c-Jun–Regulated Macrophage Activation

et al. 2014

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Clinical management of endometriosis is limited by the complex relationship between symptom severity, heterogeneous surgical presentations, and variability in clinical outcomes. As a complement to visual classification schemes, molecular profiles of disease activity may improve risk stratification to better inform treatment decisions and identify novel approaches to targeted treatment. Here, we employ a network analysis of information flow within and between inflammatory cells to discern consensus behaviors characterizing patient sub-populations. Unsupervised multivariate analysis of cytokine profiles quantified by multiplex immunoassays identified a subset of patients with a shared “consensus signature” of thirteen elevated cytokines that was associated with common clinical features, but was not observed among patient subpopulations defined by morphologic presentation alone. Enrichment analysis of consensus markers reinforced the primacy of peritoneal macrophage infiltration and activation, which was demonstrably elevated in ex vivo cultures. Although familiar targets of the NFκB family emerged among over-represented transcriptional binding sites for consensus markers, our analysis provides evidence for a previously unrecognized contribution from c-Jun, c-Fos, and AP-1 effectors of mitogen associated kinase signaling. Their crucial involvement in propagation of macrophage-driven inflammatory networks was confirmed via targeted inhibition of upstream kinases. Collectively, these analyses provide in vivo validation of a clinically relevant inflammatory network that may serve as an objective measure for guiding treatment decisions for endometriosis management, and in the future may provide a mechanistic endpoint for assessing efficacy of novel agents aimed at curtailing inflammatory mechanisms that drive disease progression.

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Section: Resultsmentioning

confidence: 95%

Molecular Network Analysis of Endometriosis Reveals a Role for c-Jun–Regulated Macrophage Activation

et al. 2014

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“…Aberrant expression of inflammatory cytokines has been found in peritoneal fluids from women with endometriosis [14], and it has been demonstrated that MBL can modulate cytokine production [15]. Furthermore, it seems that the complement system might be involved in the development of endometriosis: Tao et al [16] reported an increased expression of complement component 3 in endometriotic lesions compared to eutopic endometrium, and three more recent studies have indicated that changes in the regulation of the complement component activation are associated with endometriosis [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Mannan-binding lectin polymorphisms and serum levels in patients with endometriosis

Kruse¹,

Steffensen²,

Nielsen³

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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“…In the present study we detected a downregulation of the complement component 4A in OE (3.4-fold) and PE (1.4-fold). Down-regulation of complement components in PF has previously been demonstrated in infertile women suffering from endometriosis (22).…”

mentioning

confidence: 85%

Two-dimensional gel electrophoresis in peritoneal fluid samples identifies differential protein regulation in patients suffering from peritoneal or ovarian endometriosis

Wölfler

Meinhold‐Heerlein

Söhngen

et al. 2011

Fertility and Sterility

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Levels of complement components iC3b, C3c, C4, and SC5b-9 in peritoneal fluid and serum of infertile women with endometriosis

Cited by 34 publications

References 42 publications

Molecular Network Analysis of Endometriosis Reveals a Role for c-Jun–Regulated Macrophage Activation

Molecular Network Analysis of Endometriosis Reveals a Role for c-Jun–Regulated Macrophage Activation

Mannan-binding lectin polymorphisms and serum levels in patients with endometriosis

Two-dimensional gel electrophoresis in peritoneal fluid samples identifies differential protein regulation in patients suffering from peritoneal or ovarian endometriosis

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