Levels of Progesterone, Lh and FSH in the Plasma of Sheep During the Oestrous Cycle

Cunningham, N. F.; Symons, A. M.; Saba, N.

doi:10.1530/jrf.0.0450177

Cited by 26 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…During the luteal phase of the oestrous cycle the uterus of the ewe is exposed to high levels of circulating progesterone for about 9 days (Moore et al 1969;Thorburn et al 1969;Cunningham et al 1975). The uterus is also exposed to high plasma levels of oestradiol for periods of 2-3 days.…”

Section: Introductionmentioning

confidence: 99%

Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Stone¹,

Murphy²,

Miller³

1978

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Oestradiol and progesterone were administered alone or together in various combinations to different groups of ovariectomized ewes and several aspects of uterine and endometrial metabolism were determined. Levels of cytosol receptors for oestradiol and progesterone and metabolism of these steroids in whole uterus were measured, as well as mean cell content of RNA, rate of protein synthesis, tissue DNA concentration and alkaline phosphatase activity in the endometrium, uterine weight, and the amount of protein in uterine flushing.Oestradiol increased the level of both receptors, the RNA: DNA ratio and the rate of protein synthesis, it decreased tissue DNA concentration but it had no effect on alkaline phosphatase activity. Progesterone increased the level of the oestradiol receptor, the RNA: DNA ratio, the rate of protein synthesis and alkaline phosphatase activity, it decreased tissue DNA concentration but it did not change the level of the progesterone receptor. There were few interactions between oestradiol and progesterone. However, in ewes which received oestradiol the administration of progesterone for 9 days caused small decreases in the level of the oestradiol receptor and larger decreases in the level of the progesterone receptor. None of the hormone treatments had significant effects on oestradiol or progesterone metabolism or on the amount of protein in uterine flushings. The results are discussed in relation to the possible roles of steroid receptors in the uterus, and to changes occurring in the uterus of the intact ewe during the oestrous cycle.

show abstract

Section: Introductionmentioning

confidence: 99%

Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Stone¹,

Murphy²,

Miller³

1978

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

show abstract

“…During the first 48-72 h after the onset of oestrus (Day 0) little oestradiol or progesterone can be found in ovarian venous blood. From Day 3 until about Day 14 progesterone is secreted by the corpus luteum (Moore, Barrett, Brown, Schindler, Smith & Smyth, 1969; Thorburn, Bassett & Smith, 1969; McNatty, Revfeim «fe Young, 1973;Cunningham, Symons <& Saba, 1975). The pro-oestrous surge of oestradiol secretion occurs principally during the 36 h before the onset of behavioural oestrus, and very little proges¬ terone is secreted during this period.…”

Section: Introductionmentioning

confidence: 99%

Hormone Receptor Levels and Hormone, Rna and Protein Metabolism in the Genital Tract During the Oestrous Cycle of the Ewe

Miller¹,

Murphy²,

Stone³

1977

Journal of Endocrinology

View full text Add to dashboard Cite

The concentrations of soluble oestradiol and progesterone receptor proteins and several metabolic activities were measured in the genital tracts of ewes killed on Days 0 (oestrus), 2, 5, 10 or 14 of the oestrous cycle. In caruncular endometrium and in whole uterus the concentrations of oestradiol receptor (pmol steroid bound/mg tissue DNA) were highest at oestrus and declined steadily thereafter to minimal values at Day 14. The concentrations of progesterone receptor were highest on Days 0-5, then declined to low levels on Day 10-14. There was little metabolism of either [3H]oestradiol or [3H]progesterone in minces of whole uterus and with either steroid the pattern of metabolism did not change at any stage of the cycle. The in-vivo rates of synthesis of protein in caruncular endometrium and in isthmic oviduct were highest at or shortly after oestrus (Days 0-2), then declined to low levels on Days 10-14. RNA:DNA ratios in these two tissues were also greatest at oestrus and subsequently fell to minimal values by Day 14. The results are discussed in relation to ovarian secretion of oestradiol and progesterone during the oestrous cycle of the ewe.

show abstract

“…A series of seven repeat cultures were performed on a total of 472 COC of which 164 COCs were cultured in serum-free IVM medium containing concentrations of ovine LH (100 ng/ml) and FSH (100 ng/ml) within the range of physiological values measured during the pre-ovulatory gonadotrophin surge in sheep (Cunningham et al 1975, Narayana & Dobson 1979, Wheaton et al 1982, Atkinson et al 1989. A further 138 COCs were incubated in the serum-free IVM medium without gonadotrophins (KLH/FSH), and 170 COCs were matured in a serum-based IVM medium containing pharmacological levels of ovine LH (5 mg/ml) and FSH (5 mg/ml).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, gonadotrophin concentrations within the physiological range of levels measured for the pre-ovulatory surge in sheep were used to trigger oocyte maturation and cumulus mucification and expansion in vitro. The peak concentrations of LH and FSH measured during the preovulatory surge in sheep have been shown to range from 40 to 240 ng/ml for LH, and 5 to 350 ng/ml for FSH (Cunningham et al 1975, Narayana & Dobson 1979, Wheaton et al 1982, Atkinson et al 1989. Comparable rates of IVM and fertilisation were achieved using the physiological serum-free IVM system when compared with an established serum-based IVM system containing 10% FCS (Obrien et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of the cellular and molecular responses of ovine oocytes and their supporting somatic cells to pre-ovulatory levels of LH and FSH during in vitro maturation

2012

View full text Add to dashboard Cite

The response of Graafian follicles to pre-ovulatory surge levels of FSH and LH in vivo triggers the terminal differentiation of granulosa cells and oocyte maturation. In polyovular species, the LH-driven signalling uses the epidermal growth factor (EGF)-like ligands AREG, EREG and BTC to promote oocyte maturation and cumulus expansion. This experimental series used a physiologically relevant ovine in vitro maturation (IVM) system to evaluate the impact of exposure to pre-ovulatory levels (100 ng/ml) of LH and FSH on ovine cumulus cell expression of EGF-like ligands in vitro. The serum-free sheep IVM system supported high levels (91.4%) of gonadotrophin-induced maturation of cumulus-enclosed oocytes and embryo development to the blastocyst stage (34.5%). Results were equivalent to a serumbased IVM system (85.1% IVM, 25.8% blastocyst rate; PO0.05) but were significantly different (P!0.05) to serum-free medium without gonadotrophins (69.5% IVM; 8.0% blastocyst rate). Ovine BTC was cloned and sequenced. Gonadotrophin-induced AREG, EREG, BTC and EGFR expressions were quantified in cumulus and mural granulosa cells during IVM. A rapid induction of AREG expression was apparent in both cell types within 30 min of gonadotrophin exposure in vitro. LHCGR (LHR) was detected in mural cells and FSHR in both cumulus and mural granulosa cells. The data confirm the involvement of AREG and EGFR during gonadotrophin-induced cumulus expansion, oocyte maturation and the acquisition of developmental competence by sheep oocytes matured in vitro.

show abstract

Levels of Progesterone, Lh and FSH in the Plasma of Sheep During the Oestrous Cycle

Cited by 26 publications

References 13 publications

Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Hormone Receptor Levels and Hormone, Rna and Protein Metabolism in the Genital Tract During the Oestrous Cycle of the Ewe

Characterisation of the cellular and molecular responses of ovine oocytes and their supporting somatic cells to pre-ovulatory levels of LH and FSH during in vitro maturation

Contact Info

Product

Resources

About