Lewinella maritima sp. nov., and Lewinella lacunae sp. nov., novel bacteria from marine environments

Abstract

Two Gram-staining-negative, rod-shaped, aerobic, marine bacteria, designated HME9321T and HME9359T, were isolated from seawater and lagoon water samples in the Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus Lewinella within the family Saprospiraceae. The 16S rRNA gene sequence similarity of strain HME9321T showed highest similarities with Lewinella aquimaris HDW-36T (95.2 %), Lewinella marina MKG-38T (94.7 %) and Lewinella xylanilytica 13-9-B8T (94.0 %). Strain HME9359T had highest sequence similarities with Lewinella agarilytica SST-19T (94.7 %), Lewinella persica T-3T (94.1 %) and Lewinella antarctica IMCC3223T (93.3 %). The predominant fatty acids of strain HME9321T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and summed feature 9 (comprising iso-C16 : 0 10-methyl and/or C17 : 1ω9c) while those of strain HME9359T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and iso-C15 : 0. The major isoprenoid quinone of both strains was MK-7. Strain HME9321T contained the polar lipids, phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and nine unidentified polar lipids, while strain HME9359T contained phosphatidylethanolamine, one unidentified phospholipid and nine unidentified polar lipids. The DNA G+C contents of strains HME9321T and HME9359T were 58.7 and 62.0 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Lewinella maritima sp. nov. and Lewinella lacunae sp. nov. are proposed. The type strains are HME9321T (=KACC 17619T=CECT 8419T) and HME9359T (=KCTC 42187T=CECT 8679T), respectively.

Bacterial Communities in the Rhizosphere and Phyllosphere of Halophytes and Drought-Tolerant Plants in Mediterranean Ecosystems

“…On the other hand, the taxa Marinobacter, Gracilimonas, Lewinella and Jannaschia have been detected in >20% of the sample's reads only in the saline environment of the National Park of Delta Axios, and similarly can be characterized as indicator taxa for similar ecosystems, when in high abundances. These taxa have all been commonly isolated from marine sediments and high-salinity environments [87][88][89][90] and are considered characteristic halotolerant bacteria. Stressed environments overall can play critical roles in structuring the plant bacterial communities by selecting stress tolerant groups of microbes not necessarily correlated directly to the vegetation of the ecosystem [82].…”

Lewinella aurantiaca sp. nov., a carotenoid pigment-producing bacterium isolated from surface seawater

“…At the time of writing, the genus Lewinella comprises 12 species with validly published names (https://lpsn.dsmz.de/genus/lewinella) [4]. Members of the genus Lewinella have been isolated from different sources such as brown mud, beach sediment, beach sand, lagoon, seawater, marine sand, Antarctica coastal seawater and sea snail [1, 5–10]. Lewinella species are Gram-stain-negative, aerobic, orange- or yellow-pigmented, devoid of flagella, motile by gliding or non-motile, chemoheterotrophic, rod-, ovoid- or filament-shaped bacteria that contain MK-7 as the predominant menaquinone and phosphatidylethanolamine as the major polar lipid.…”

Portibacter marinus sp. nov., isolated from the sediment on the surface of plastics and proposal of a novel genus Neolewinella gen. nov. based on the genome-based phylogeny of the family Lewinellaceae

“…The description is as for Lewinella maritima [23]. The type strain is HME9321 T (=KACC 17619 T =CECT 8419 T ).…”

“…Additionally, the cellular fatty acid composition of L. cohaerens DSM 23179 T also demonstrated a clearly different pattern from other Lewinella species (Table4). L. cohaerens DSM 23179 T had a large amount of iso-C 15 : 1 F and a minor amount of summed feature 3, in comparison with L. aurantiaca SSH13 T , L. persica DSM 23188 T , L. agarilytica KCTC 12774 T , L. marina NBRC 102633 T and L. litorea HSMS-39 T[21][22][23]. The major respiratory quinone of the new genus Neolewinella is MK-7, similar to the genus Lewinella and other genera of the family Lewinellaceae.…”