We present a sustainable fabrication method for cheap point-of-care microfluidic systems, employing hot embossing of natural shellac as a key feature of an energyefficient fabrication method that exclusively uses renewable materials as consumables. Shellac is a low-cost renewable biomaterial that features medium hydrophilicity (e.g., a water contact angle of ca. 73 ) and a high chemical stability with respect to common solvents such as cyclohexane or toluene, rendering it an interesting candidate for low-cost microfluidics and a competitor to well-known systems such as paperbased or polydimethylsiloxane-based microfluidics. Moreover, its high replication accuracy for small features down to 30 lm lateral feature size and its ability to form smooth surfaces (surface roughness R a ¼ 29 nm) at low embossing temperatures (glass transition temperature T g ¼ 42.2 C) enable energy-efficient hot embossing of microfluidic structures. Proof-of-concept for the implementation of shellac hot embossing as a green fabrication method for microfluidic systems is demonstrated through the successful fabrication of a microfluidic test setup and the assessment of its resource consumption. Published by AIP Publishing. [http://dx