Ligand-binding activity of growth hormone receptor (GH-R) in bulls of different breeds with identified GH-R genotypes

Abstract

The relationship between the ligand-binding activity of growth hormone receptor (GH-R) and polymorphism \nAlul, Accl and Stul sites in the 5' flanking region of bovine GH-R gene was examined. The study was performed on twenty six 15-month old bulls of dairy (Friesians) and beef breeds (Charolais, Limousine, Piemontese, Aberdeen Angus, Hereford). Receptor binding capacity (B max ) and dissociation constant (K d ) for GH-R were determined by the Scatchard method. The polymorphism in the GH-R gene was analyzed by PCR-RFLP technique. Our results showed significant differences in the liver GH-R B and K. between the dairy and beef breeds. B was greater (P<0.05)in Polish Friesians as compared to the beef breeds, while K d revealed the lowest figure in the dairy breed (P<0.01). No significant differences were found in the B max and K d values between genotypes within the 5' flanking region of bovine GH-R gene. In conclusion, ligand-binding activity of the liver GH receptors was shown to differ between the dairy and beef bulls. Furthermore, the polymorphism in the 5' regions of GH-R gene seems to have no influence on ligand-binding properties of the liver GH receptor in cattle.

Polymorphism in the 5’‑noncoding region of the bovine growth hormone receptor gene and its association with meat production traits in cattle

“…milk yield and composition, are correlated with the polymorphism of GHR [1,6]. Moreover, we found significant differences in the ligand binding parameters to liver GHR (dissociation constant -K d ) between RFLP-AluI and -AccI genotypes within the 5'-flanking region of the bovine GHR gene in Polish Friesians [11].…”

Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia

“…Effects of GH on growth are observed in several tissues, including bone, muscle and adipose tissue. Therefore is a great interest in using GH gene as a promising candidate for selection purposes in breeding program of animals (Grochowska et al, 2002). GH gene in cattle is located on chromosome 19 consisting an arranged 191 amino acids (Hediger et al, 1990;Schlee et al, 1994), it has 2856 bp of nucleotide and consists of five exons and four introns (Woychik et al, 1982;Gordon et al, 1983;Vukasinovic et al, 1999).…”

Single nucleotide polymorphism (SNP) in the 5'-noncoding region of the bovine growth hormone receptor gene and its association with dairy production traits in Polish Black-and-White cattle

“…The RFLPs at AluI and AccI sites were estimated as previously described (Grochowska et al, 2002). To genotype GHR gene at Fnu4HI site the following primers were used to amplify the 836-bp fragment (-1866 to -1031 nt): forward -5'-TGCGTGCACAGCAGCTCAACC-3'; reverse -5'-AGCAACCCCACTG-CTGGGCAT-3'.…”

“…milk yield and composition, are correlated with polymorphism of GHR (Falaki et al, 1996;Aggrey et al, 1999). Significant differences in the ligand binding parameters to the liver GHR were found between dairy and beef breeds (Grochowska et al, 2002); B max (receptor binding capacity) was greater in Friesians as compared to beef breeds, while K d (ligand dissociation constant) revealed the lowest figure in the dairy breed. A difference was also found in the K d value between RFLP-AluI and -AccI genotypes within the 5'-flanking region of bovine GHR gene in Polish Friesians.…”