Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars

Abstract

The aim of this work was to evaluate the effect of fi ve light qualities [fl uorescent lamps (FL); white (WL), red (RL), blue (BL), or red/blue LEDs (RBL)] and two types of fl ask sealing (with or without porous membranes) in the in vitro germination and growth of two Capsicum frutescens cultivars (Espaguetinho and Cayenne). For this, two seeds were inoculated per fl ask (350 mL) containing 50 mL of MS medium with 100 mg L -1 myo-inositol, 30 g L -1 sucrose, 6.0 g L -1 agar, and pH 5.8 ± 0.1. The plants were kept under culture room conditions (16-h photoperiod, 25 ± 2°C and irradiance of 40 μmol m -2 s -1 ).The experiment was arranged in CRD, 2x5x2 factorial scheme (Cultivar X Light qualities X Sealing type). At 42 days, the following traits were evaluated: percentage of germination; root and shoot length; number of green leaves, senescent leaves and nodal segments; and fresh and dry weight. Plants grown under RL and BRL, showed greater number of green leaves, and longer shoots and roots. The use of porous membranes remarkably reduced the number of senescent leaves. Thus, the use of porous membranes, RL and BRL are recommended since they improve growth and development of pepper in vitro.

Led enhance the growth characteristics and affects the ex vitro aclimatization of sugarcane

“…For the sealing types, flasks without membranes (28.00) increased this number of leaves (Figure 2B). Similar results were observed in Capsicum frutescens cultivars that is, plants under red LEDs and blue/ red LEDs and flasks without membranes produced more green leaves (Gris et al, 2021).…”

“…Under these conditions, CO 2 concentrations in cultivation flasks are generally low, and together with other factors, they can cause physiological stress to plants which directly affects morphogenesis and growth (Xiao;Niu;Kozai, 2011). To overcome these restrictions and improve the in vitro culture environment, gas-porous membranes have been used (Gris et al, 2021), and are capable of allowing gas exchange between the in vitro and ex vitro environments, providing a reduction in relative humidity inside the culture flasks, an increase in transpiration and absorption of nutrients and water by plants, which favors photosynthesis and improves rates growth (Kozai, 2010;Xiao;Niu;Kozai, 2011).…”

“…Several studies report the advantage of using natural ventilation and forced ventilation with CO 2enrichment. For natural ventilation, studies with Pfaffia glomerata (Iarema et al, 2012;Saldanha et al, 2012;Corrêa et al, 2015a;Corrêa et al, 2015b), Capsicum annuum (Batista et al, 2017), Physalis angulata (Santos et al, 2020), Acca sellowiana (Caetano et al, 2021), Capsicum frutescens (Gris et al, 2021), Epidendrum fulgens (Fritsche;Pinheiro;Guerra, 2022), Guazuma ulmifolia (Jesus-Santana et al, 2022), for forced ventilation, studies with Pfaffia glomerata (Saldanha et al, 2013;Saldanha et al, 2014;Ferreira et al, 2019), Lippia alba (Batista et al, 2017), Etlingera elatior (Pinheiro et al, 2021), among others. These studies brought the benefits of the use of gas-porous membranes, which provides a reduction in relative humidity and ethylene gas inside the flasks, which can eliminate or drastically reduce morphophysiological disorders and increase survival during acclimatization.…”