Light spectra affect the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by changing the protein profile and polyamine contents

Oliveira, Tadeu dos Reis de; Aragão, Victor Paulo Mesquita; Moharana, Kanhu C.; Fedosejevs, Eric T.; Amaral, Fernanda Plucani do; Sousa, Kariane Rodrigues de; Thelen, Jay J.; Venâncio, Thiago M.; Silveira, Vanildo; Santa–Catarina, Claudete

doi:10.1016/j.bbapap.2020.140529

Cited by 22 publications

(15 citation statements)

References 64 publications

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“…Wavelength, photoperiod, and flux density have a significant impact on In Vitro morphogenesis, photosynthesis, and phototropism [129] and require further study in Cannabis. Different species have different responses to light conditions [129,130]. Some plants may respond positively to the addition of photosynthetic photon flux, par-ticularly under mixotrophic/photoautotrophic conditions (CO 2 -rich and low sugar level).…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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“…Among the auxins commonly used in tissue culture, IBA has been widely used due to low explant phytotoxicity, providing positive results for in vitro rooting, as demonstrated for Caryocar brasiliense (Santos et al, 2006), Ocotea porosa (Pelegrini et al, 2011) and Parapiptadenia rigida (Kielse et al, 2009). However, in general, responses are dependent on genetic material and are influenced by culture medium composition, auxin type and concentrations (Oliveira;Dias;Brondani, 2013).…”

Section: Auxin Treatmentsmentioning

confidence: 99%

In vitro multiplication of Cedrela fissilis Vell.: A threatened brazilian hardwood forest tree

Bonfá

Nascimento

Werner

2021

PCC&M

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Cedrela fissilis Vell. is a valuable wood species from Atlantic Forest and is at risk of extinction. Therefore, a protocol for clonal multiplication was developed for C. fissilis using different explants from in vitro seedlings. The seeds were germinated in total or half-strength MS and B5 basal media. The half-strength MS medium was chosen based on its high germination rate (82 %) and germination speed index (1.218). Shoot tips, nodal segments and cotyledonary nodes explants were extracted from the in vitro seedlings and cultivated for shoot induction in MS medium supplemented with KIN or BAP (0, 0.5, 2 and 4 μM). The highest number (2.2) and length (3.84 cm) of shoots were obtained from cotyledonary nodes cultured in the absence of cytokinin. For rooting, the MS medium was supplemented with IBA or NAA (0, 1 and 3 μM). Rooting was induced in all auxin treatments. The highest number of roots per explant was observed in the treatment supplemented with 3μM IBA (2.54). The present study provides in vitro shoot multiplication and root regeneration systems for C. fissilis, with great potential to be used for micropropagation, germplasm preservation and genetic transformation.

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“…Mature seeds of C. ssilis obtained from Caiçara Comércio de Sementes LTDA located in Brejo Alegre (21°10'S and 50°10'W), São Paulo State, Brazil, were germinated in vitro according to Oliveira et al (2020). Sixty-day-old seedlings were used as the source of apical and cotyledonary nodal segment explants to obtain the shoots that were used for rooting in each subculture.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In vitro germination was performed according to Oliveira et al (2020). First, the seeds were surface disinfected and then transferred to glass test tubes (15 x 2.5 cm; Laborglas; São Paulo, Brazil) containing 10 mL of MS (Murashige and Skoog 1962) culture medium (M519; Phytotechnology Lab; Lenexa, USA) supplemented with 20 g L -1 sucrose (Vetec; Rio de Janeiro, Brazil) and 2 g L -1 Phytagel (Sigma-Aldrich; St. Louis, USA).…”

Section: In Vitro Germinationmentioning

confidence: 99%

Long-term subculture affects rooting competence via changes in the hormones and protein profiles in Cedrela fissilis Vell. (Meliaceae) shoots

Oliveira

Balfagón

Sousa

et al. 2021

Plant Cell Tiss Organ Cult

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Long-term subculture plays an essential role in the large-scale multiplication and production of somatic plantlets. We investigated the effects of long-term subculture on in vitro shoot development and ex vitro rooting associated with changes in the hormones and protein pro les in C. ssilis. The number of subcultures of shoots induced a decrease in the ex vitro rooting response. The reduction in adventitious root (AR) formation was associated with decreases in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), 12-oxo phytodienoic acid (OPDA), putrescine (Put), and spermine and increases in jasmonic acid (JA), jasmonoyl-isoleucine, trans-cinnamic acid, and salicylic acid contents in shoots at the fourth subculture compared to the rst. The ornithine decarboxylase enzyme preferentially functions in the Put biosynthesis pathway and was related to the highest AR formation in shoots at the rst subculture. Down-accumulation of the auxin-binding protein ABP19a in shoots from the fourth subculture compared to the rst subculture was related to a decrease in both IAA contents and AR formation. In addition, downaccumulation of glucose-6-phosphate isomerase, glutamine synthetase leaf isozyme chloroplastic, 5methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, L-ascorbate peroxidase, cytosolic, monodehydroascorbate reductase, and 2-Cys peroxiredoxin BAS1-like, chloroplastic and up-accumulation of caffeoyl-CoA O-methyltransferase 1 and isoforms of peroxidase 4 proteins in shoots from the fourth relative to the rst subculture were associated with a reduction in AR formation. These results showed that the understanding of hormonal and molecular mechanisms related to the potential of AR formation in shoots under successive subcultures is relevant to improving large-scale plantlet production in C. ssilis. Key MessageLong-term subculture affects the competence to ex vitro rooting of Cedrela ssilis shoots by changes in endogenous hormones and protein pro les.

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Light spectra affect the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by changing the protein profile and polyamine contents

Cited by 22 publications

References 64 publications

The Past, Present and Future of Cannabis sativa Tissue Culture

The Past, Present and Future of Cannabis sativa Tissue Culture

In vitro multiplication of Cedrela fissilis Vell.: A threatened brazilian hardwood forest tree

Long-term subculture affects rooting competence via changes in the hormones and protein profiles in Cedrela fissilis Vell. (Meliaceae) shoots

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