Lignin degradation by water buffalo

Xu, Qingbiao; Hui-min, Zhong; Zhou, Jiayan; Wu, Yanjuan; Ma, Zhenhua; Yang, Liguo; Wang, Zhen; Ling, Chuqi; Li, Xiang

doi:10.1007/s11250-021-02787-z

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…In addition to biodegradable fiber, the results also proved the ADL degradability in eight kinds of roughage by buffalo. The above results are consistent with Xu and Zhong's study [3]. They brought forward that buffalo had lignin degradation by comparing buffalo and cattle and found that the ADL degradation rate of rice straw in buffalo rumen was significantly higher than that in cattle rumen.…”

Section: Discussionsupporting

confidence: 90%

“…Xu and Zhong found that when ingesting the same forage with high fiber and low protein, buffalo showed higher dry mater intake and stronger growth performance than cattle. By conducting comparative experiments between buffalo and cattle, they reported that buffalo could degrade lignin efficiently but cattle cannot [3]; however, the studies focusing on this area are scarce. In rumen, lignin limits microbial enzymatic access to lignocellulose, which affects roughage digestibility [4] and closely disturbs the utilization extent.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

et al. 2021

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The water buffalo (Bubalus bubalis) is known for its unique utilization of low-quality fibrous feeds and outstanding digestion performance, highlighting its role as an animal model in studying fiber fractions degradation. Among roughage, lignin attracted wide attention in ruminant nutrition studies, which affects animal digestibility. Therefore, the present study aims to investigate the functional relation between three lignin monomeric compositions of coniferyl alcohol (G), ρ-coumaryl alcohol (H) and sinapyl alcohol (S) and ruminal fiber degradation in water buffalo. Hence, three female water buffaloes (Nili-Ravi × Mediterranean, five years old, 480 ± 20 kg) were assigned for an in vivo study by utilizing the nylon-bag method, examining eight kinds of roughage. All the experimental roughage types were analyzed for the effective degradability (ED) of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL) and hemicellulose (HC) fractions. Then, prediction models for the roughage fiber degradation were established based on the characteristics of lignin monomer content. The results showed that S, S/G and S/(G+S+H) were positively correlated with the ED of NDF, ADF, CEL and HC; H/S was negatively correlated. For the effective degradability of ADL (ADLD), S and S/(G+S+H) were positively correlated with it; H, H/G, H/S and H/(G+S+H) were negatively correlated. The model with the highest fitting degree was ADLD = 0.161 − 1.918 × H + 3.152 × S (R2 = 0.758, p < 0.01). These results indicated that the lignin monomer composition is closely related to the utilization rate of roughage fiber. S-type lignin monomer plays a vital role in the fiber degradation of roughage. The experiment found the effect of lignin monomer composition on the degradation of fiber fractions using buffalo as the experimental animal and constructed prediction models, providing a scientific basis for building a new technological method using lignin composition to evaluate buffalo roughage. Furthermore, the capacity of ADL degradation of buffalo was proved in this experiment. In order to further explore the ability of lignin degradation by the buffalo, the DNA of rumen microorganisms was extracted for sequencing. The top three composition of rumen microorganisms at the genus level were Prevotella_1, 226, Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG-011. Six strains with lignin degradation ability were screened from buffalo rumen contents. This experiment also revealed that the buffalos possess rumen microorganisms with lignin degradation potential.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

et al. 2021

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“…Buffalo exhibited greater in situ ruminal degradation of straw than Holstein, as indicated by greater a and ED of DM and NDF, together with greater c of fraction b in our study. Xu et al (2021) reported that buffalo exhibit greater in situ ruminal fiber degradation of rice straw than other cattle. In vitro ruminal batch culture indicates that inoculating rumen fluid from buffalo has greater NDF and ADF degradation of steam-treated sugarcane than that from Holstein cattle ( Jabari et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Buffalo exhibits better adaptation to hot and humid tropics than other cattle for their greater resistance to heat, certain diseases, and pests. In addition, buffalo also have a greater ability to digest roughage, especially when the roughage is of low quality ( Hussain and Cheeke, 1996 ; Xu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of in situ ruminal straw fiber degradation and bacterial community between buffalo and Holstein fed with high-roughage diet

Zhang

et al. 2023

Front. Microbiol.

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Buffalo exhibits great efficiency in utilizing low-quality roughage, which can be due to the combined effect of host physiological feature and roughage diet fed. The present study was designed to compare the ruminal fiber degradation and the bacterial community attached to straws in buffalo and Holstein when fed with the same high-roughage diet using in situ ruminal incubation technique. Rice and wheat straws were selected as the incubation substrates and sampled at 0, 4, 12, 24, 48, 72, 120, and 216 h of incubation time to measure the kinetics of dry matter (DM) and neutral detergent fiber (NDF) disappearance. Additional two bags were incubated and sampled at 4 and 48 h of incubation time to evaluate the bacterial community attached to straws. The results showed that buffalo exhibited a greater (p ≤ 0.05) fraction of rapidly soluble and washout nutrients and effective ruminal disappearance for both DM and NDF of straw than Holstein, together with a greater (p ≤ 0.05) disappearance rate of potentially degradable nutrient fraction for NDF. Principal coordinate analysis indicated that both host and incubation time altered the bacterial communities attached to straws. Buffalo exhibited greater (p ≤ 0.05) 16S rRNA gene copies of bacteria and greater (p ≤ 0.05) relative abundance of Ruminococcus attached to straw than Holstein. Prolonging incubation time increased (p ≤ 0.05) the 16S rRNA gene copies of bacteria, and the relative abundance of phyla Proteobacteria and Fibrobacters by comparing 4 vs. 48 h of incubation time. In summary, buffalo exhibits greater ruminal fiber degradation than Holstein through increasing bacterial population and enriching Ruminococcus, while prolonging incubation time facilitates fiber degradation through enriching phyla Proteobacteria and Fibrobacteres.

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“…Buffaloes were found to have higher digestibilities of DM, crude protein, and organic matter in rice straw than cattle (Terramoccia et al, 2000 ; Chanthakhoun et al, 2012 ). Interestingly, buffalo was the first mammal to be verified to have the ability of lignin degradation (Xu et al, 2021 ). According to a prior study, strains with lignin degradation ability were isolated from buffalo rumen, and the degradation rate of sodium lignosulfonate could reach 11.1% (Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of ligninolytic bacterium (Bacillus cereus) from buffalo (Bubalus bubalis) rumen and its effects on the fermentation quality, nutrient composition, and bacterial community of rape silage

et al. 2023

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This study aimed to isolate and identify a ligninolytic bacterium from the rumen of buffalo (Bubalus bubalis) and investigate its effects as a silage additive for whole-plant rape. Three lignin-degradation strains were isolated from the buffalo rumen, with AH7-7 being chosen for further experiments. Strain AH7-7, with acid tolerance and a 51.4% survival rate at pH 4, was identified as Bacillus cereus. It exhibited a lignin-degradation rate of 20.5% after being inoculated in a lignin-degrading medium for 8 days. We divided the rape into four groups according to the various additive compositions to examine the fermentation quality, nutritional value, and bacterial community after ensiling: Bc group (inoculated with B. cereus AH7-7 3.0 × 106 CFU g FW−1), Blac group (inoculated with B. cereus AH7-7 1.0 × 106 CFU g FW−1, L. plantarum 1.0 × 106 CFU g FW−1, and L. buchneri 1.0 × 106 CFU g FW−1), Lac group (inoculated with L. plantarum 1.5 × 106 CFU g FW−1 and L. buchneri 1.5 × 106 CFU g FW−1), and Ctrl group (no additives). After 60 days of fermentation, the application of B. cereus AH7-7 was potent in modulating the fermentation quality of silage, especially when combined with L. plantarum and L. buchneri, as indicated by lower dry matter loss and higher contents of crude protein, water-soluble carbohydrate, and lactic acid. Furthermore, treatments with the B. cereus AH7-7 additive decreased the contents of acid detergent lignin, cellulose, and hemicellulose. The B. cereus AH7-7 additive treatments reduced the bacterial diversity and optimized the bacterial community compositions of silage, with an increase in the relative abundance of beneficial Lactobacillus and a decrease in the relative abundance of undesirable Pantoea and Erwinia. Functional prediction revealed that inoculation with B. cereus AH7-7 could increase the cofactors and vitamins metabolism, amino acid metabolism, translation, replication and repair, and nucleotide metabolism, while decreasing the carbohydrate metabolism, membrane transport, and energy metabolism. In brief, B. cereus AH7-7 improved the microbial community, fermentation activity, and ultimately the quality of silage. The ensiling with B. cereus AH7-7, L. plantarum, and L. buchneri combination is an effective and practical strategy to improve the fermentation and nutrition preservation of rape silage.

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Lignin degradation by water buffalo

Cited by 8 publications

References 24 publications

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

Comparison of in situ ruminal straw fiber degradation and bacterial community between buffalo and Holstein fed with high-roughage diet

Isolation and identification of ligninolytic bacterium (Bacillus cereus) from buffalo (Bubalus bubalis) rumen and its effects on the fermentation quality, nutrient composition, and bacterial community of rape silage

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