Lignin peroxidase from Chrysonilia sitophila: Heat-denaturation kinetics and pH stability

Ferrer, Irene; Espósito, Elisa; Durán, Nélson

doi:10.1016/0141-0229(92)90010-l

Cited by 23 publications

(5 citation statements)

References 13 publications

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“…P. chrysosporium and other basidiomycetes are known to produce laccases, lignin peroxidases, manganese peroxidase and versatile peroxidases which would all contribute to the degradation of the various lignin linkages in cocopeat. Although ascomycetes such as Neurospora primarily produce laccases and lignin peroxidases [15,16,26,27], the extremely rapid growth of N. discreta seems to compensate, thus resulting in lignin degradation in cocopeat similar to that by P.chrysosporium.…”

Section: Carbon Utilizationmentioning

confidence: 99%

Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Pamidipati

Ahmed

2016

Appl Biochem Biotechnol

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Locally isolated fungus, Neurospora discreta, was evaluated for its ability to degrade lignin in two agricultural residues: cocopeat and sugarcane bagasse with varying lignin concentrations and structures. Using Klason's lignin estimation, high-performance liquid chromatography, and UV-visible spectroscopy, we found that N. discreta was able to degrade up to twice as much lignin in sugarcane bagasse as the well-known white rot fungus Phanerochaete chrysosporium and produced nearly 1.5 times the amount of lignin degradation products in submerged culture. Based on this data, N. discreta is a promising alternative to white rot fungi for faster microbial pre-treatment of agricultural residues. This paper presents the lignin degrading capability of N. discreta for the first time and also discusses the difference in biodegradability of cocopeat and sugarcane bagasse as seen from the analysis carried out using Fourier transform infrared spectroscopy.

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Section: Carbon Utilizationmentioning

confidence: 99%

Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Pamidipati

Ahmed

2016

Appl Biochem Biotechnol

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“…radiata [36]. Ferrer et al [37] reported on the characterization of three major LiP isozymes (LIG-I, LIG-II, and LIG-III) from the ascomyete, Chrysonilia sitophila. The molecular masses of LIG-I, LIG-II, and LIG-III were 68, 50, and 47 kDa, respectively.…”

Section: Lips M Other Wood-rotting Fungimentioning

confidence: 99%

Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium

Reddy¹

1994

FEMS Microbiology Reviews

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The white-rot basidiomycete Phanerochaete chrysosporium produces lignin peroxidases (LiPs), a family of extracellular glycosylated heme proteins, as major components of its lignin-degrading system. Up to 15 LiP isozymes, ranging in M(r) values from 38,000 to 43,000, are produced depending on culture conditions and strains employed. Manganese-dependent peroxidases (MnPs) are a second family of extracellular heme proteins produced by P. chrysosporium that are also believed to be important in lignin degradation by this organism. LiP and MnP production is seen during secondary metabolism and is completely suppressed under conditions of excess nitrogen and carbon. Excess Mn(II) in the medium, on the other hand, suppresses LiP production but enhances MnP production. Nitrogen regulation of LiP and MnP production is independent of carbon and Mn(II) regulation. LiP activity is also affected by idiophasic extracellular proteases. Intracellular cAMP levels appear to be important in regulating the production of LiPs and MnPs, although LiP production is affected more than MnP production. Studies on the sequencing and characterization of lip cDNAs and genes of P. chrysosporium have shown that the major LiP isozymes are each encoded by a separate gene. Each lip gene encodes a mature protein that is 343-344 amino acids long, contains 1 putative N-glycosylation site, a number of putative O-glycosylation sites, and is preceded by a 27-28-amino acid leader peptide ending in a Lys-Arg cleavage site. The coding region of each lip gene is interrupted by 8-9 introns (50-63 bp), and the positions of the last two introns appear to be highly conserved. There are substantial differences in the temporal transcription patterns of the major lip genes. The sequence data suggest the presence of three lip gene subfamilies. The genomic DNA of P. chrysosporium strain BKMF-1767 was resolved into 10 chromosomes (genome size of 29 Mb), and that of strain ME-446 into 11 chromosomes (genome size of 32 Mb). The lip genes have been localized to five chromosomes in BKMF-1767 and to four chromosomes in ME-446. DNA transformation studies have reported both integrative and non-integrative transformation in P. chrysosporium.

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“…Dentre outros resultados, destaca-se a degradação de efluentes derivados do processo de branqueamento de polpa, utilizando-se lignina peroxidase (de Phanerochaete chrysosporium) imobilizada em resinas de troca iônica 70 . Bons resultados têm sido obtidos na imobilização de peroxidase de raiz forte e lignina peroxidase (de Chrysonilia sitophila) em Sepharose [71][72][73] e lacase em alginato 74 . Este último estudo reporta uma eficiência significativamente maior dos sistemas enzimáticos imobilizados.…”

Section: Processos Enzimáticosunclassified

Novas tendências para o tratamento de resíduos industriais contendo espécies organocloradas

Freire¹,

Pelegrini²,

Kubota³

et al. 2000

Quím. Nova

Self Cite

146

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, and mainly the low efficiency of the current remediation processes, are important aspects that have been promoted to study new degrading technologies. Among the great number of new physical and chemical alternatives, the photochemical and electrochemical processes have been played an important role, mainly due to their high degradation capacity through relatively low-cost procedures. In these contexts biological processes, the use of white-rot fungi, or even ligninolytic enzymes produced from them, are also very promising alternatives. However, the necessity of long reaction time and the high cost of the enzyme production process are some of the drawbacks that difficult the definitive consolidation of these processes.Keywords: organochloride compounds; industrial effluents; degradation processes. REVISÃO INTRODUÇÃOCom a evolução dos processos industriais e o conseqüente surgimento de inúmeros produtos que rapidamente tornaram-se de primeira necessidade, a atividade industrial adquiriu um caráter essencial na sociedade contemporânea. Embora a sua importância seja indiscutível, a atividade industrial costuma ser responsabilizada, e muitas vezes com justa razão, pelo fenô-meno de contaminação ambiental, principalmente graças a dois fatores de extrema importância: a) o acúmulo de matérias primas e insumos, que envolve sérios riscos de contaminação por transporte e disposição inadequada; e b) ineficiência dos processos de conversão, o que necessariamente implica a geração de resíduos.Embora exista uma preocupação universal em se evitar episódios de contaminação ambiental, estes eventos prejudiciais continuam acontecendo, principalmente porque, em função dos fatores acima comentados, grande parte dos processos produtivos são intrinsecamente poluentes. Ao longo das décadas, a atividade industrial tem produzido rejeitos gasosos, líquidos e sólidos nocivos ao meio ambiente. Substâncias químicas presentes na atmosfera, principalmente compostos organoclorados voláteis produzidos pelo homem, tem colocado em risco a vida na terra através da destruição da camada de ozônio. Da mesma forma, processos industriais que utilizam grandes volumes de água contribuem significativamente com a contaminação dos corpos d'água, principalmente pela ausência de sistemas de tratamento para os grandes volumes de efluentes líquidos produzidos. Dentro deste contexto, uma importante parcela do processo de contaminação pode ser atribuída às atividades das refinarias de petróleo, indústrias químicas, têxteis e papeleiras. No entanto, não menos importante é a contribuição da atividade agrícola, dos esgotos sanitários e dos resíduos domésticos.Em função deste panorama, muitos estudos têm sido realizados buscando desenvolver tecnologias capazes de minimizar o volume e a toxicidade dos efluentes industriais [1][2][3][4][5] . Infelizmente, a aplicabilidade destes tipos de sistemas está subordinada ao desenvolvimento de processos modificados e ao estabelecimento de sistemas de reciclagem de efluentes, atividades que implicam em tecnologias evolutivas e ...

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Lignin peroxidase from Chrysonilia sitophila: Heat-denaturation kinetics and pH stability

Cited by 23 publications

References 13 publications

Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium

Novas tendências para o tratamento de resíduos industriais contendo espécies organocloradas

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