LINC01232 Sponges Multiple miRNAs and Its Clinical Significance in Pancreatic Adenocarcinoma Diagnosis and Prognosis

Du, Wei; Lei, Chengbin; Wang, Yanzhen; Ding, Yiwen; Tian, Peng

doi:10.1177/1533033820988525

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Non-coding RNAs (ncRNAs) are key components of the transcriptome and play important roles in both normal biological activity and pathological processes, such as viral infection and tumorigenesis [ 19 ]. Previous studies have demonstrated that LINC01232 exerts an oncogenic role in numerous types of tumors [ 8 , 9 ]. In this study, we found that the expression of LINC01232 was upregulated in STAD tissues and gastric cancer lines, indicating that LINC01232 may be a functional gene and a molecular target for clinical treatment of gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

“…LncRNA has a variety of biological functions in all stages of cancer development, including cell development and immunity, cell proliferation and differentiation, and apoptosis regulation [ 7 ]. Previous evidence has indicated that long intergenic nonprotein-coding RNA 1232 (LINC01232) exerts an oncogenic role in numerous types of tumors, including pancreatic adenocarcinoma, esophageal squamous cell carcinoma, et al , [ 8 , 9 ]. In this study, we pre-analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool.…”

Section: Introductionmentioning

confidence: 99%

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LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2

Liu

et al. 2021

J. Microbiol. Biotechnol.

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To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2

Liu

et al. 2021

J. Microbiol. Biotechnol.

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“…Analysis of the TCGA database and clinical tissues showed that the expression of LINC01232, TM9SF2, and EIF4A3 is upregulated in PAAD. LINC01232 is an lncRNA that is highly expressed in PAAD and has been associated with poor prognosis ( 47 ). However, no correlation between EIF4A3 and LINC01232 expression has been identified, suggesting that EIF4A3 might be recruited by LINC01232.…”

Section: Eif4a3 Acts As An Oncogene In Malignant Tumorsmentioning

confidence: 99%

Eukaryotic Initiation Factor 4A-3: A Review of Its Physiological Role and Involvement in Oncogenesis

She

Liu

et al. 2021

Front. Oncol.

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EIF4A3, a member of the DEAD-box protein family, is a nuclear matrix protein and a core component of the exon junction complex (EJC). Under physiological conditions, EIF4A3 participates in post-transcriptional gene regulation by promoting EJC control of precursor mRNA splicing, thus influencing nonsense-mediated mRNA decay. In addition, EIF4A3 maintains the expression of significant selenoproteins, including phospholipid hydroperoxide glutathione peroxidase and thioredoxin reductase 1. Several recent studies have shown that EIF4A3 promotes tumor growth in multiple human cancers such as glioblastoma, hepatocellular carcinoma, pancreatic cancer, and ovarian cancer. Molecular biology studies also showed that EIF4A3 is recruited by long non-coding RNAs to regulate the expression of certain proteins in tumors. However, its tumor-related functions and underlying mechanisms are not well understood. Here, we review the physiological role of EIF4A3 and the potential association between EIF4A3 overexpression and tumorigenesis. We also evaluate the protein’s potential utility as a diagnosis biomarker, therapeutic target, and prognosis indicator, hoping to provide new ideas for future research.

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“…As a novel lncRNA, long intergenic non‐protein coding RNA 1232 (LINC01232) has been validated to exert function in the development and progression of human cancers, such as pancreatic cancer (Du et al., 2021; Li et al., 2019; Meng et al., 2020), esophageal squamous cell carcinoma (Zhao et al., 2020), and gastric cancer (Liu et al., 2021). Nonetheless, the specific function of LINC01232 in BC remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNA long intergenic non‐protein coding RNA 1232 promotes cell proliferation, migration and invasion in bladder cancer via modulating miR‐370‐5p/PIM3 axis

Feng

Yang

Chen

et al. 2022

J Tissue Eng Regen Med

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Increasing evidences have suggested that long non‐coding RNAs are critical regulators in the progression of tumor growth. Long intergenic non‐protein coding RNA 1232 (LINC01232) was verified as an oncogene in multiple cancers. Nevertheless, its function in bladder cancer (BC) remains to be uncovered. In the current study, we detected LINC01232 expression utilizing quantitative real‐time polymerase chain reaction (RT‐qPCR) and discovered that LINC01232 was overexpressed in BC cell lines versus normal cell line. Besides, the effect of LINC01232 on BC cell behaviors was measured by colony formation, Cell Counting Kit‐8 (CCK‐8), transwell, TdT‐mediated dUTP Nick‐End Labeling and caspase‐3/8 activity assays. Functionally, LINC01232 deficiency suppressed cell proliferation, migration and invasion. Next, miR‐370‐5p was proved to bind with LINC01232 by RNA pull down, RNA‐binding protein immunoprecipitation (RIP) and luciferase reporter assays. Furthermore, PIM3 expression was negatively modulated by miR‐370‐5p and markedly increased in BC cell lines. Moreover, PIM3 silence repressed proliferation, migration and invasion but triggered apoptosis of BC cells. The rescue assays validated that upregulation of PIM3 recovered the effects of LINC01232 silence on the growth of BC cells. To summarize, our study manifested that LINC01232 accelerates BC progression by targeting miR‐370‐5p/PIM3 axis. Targeting LINC01232 might offer novel insight into BC treatment.

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LINC01232 Sponges Multiple miRNAs and Its Clinical Significance in Pancreatic Adenocarcinoma Diagnosis and Prognosis

Cited by 7 publications

References 32 publications

LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2

LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2

Eukaryotic Initiation Factor 4A-3: A Review of Its Physiological Role and Involvement in Oncogenesis

Long non‐coding RNA long intergenic non‐protein coding RNA 1232 promotes cell proliferation, migration and invasion in bladder cancer via modulating miR‐370‐5p/PIM3 axis

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