Linked influence of pH and cations on fatty-acid monolayer integrity related to high-quality Langmuir-Blodgett films

Leonard, Mark; Morelis, Renée; Coulet, Pierre R.

doi:10.1016/0040-6090(95)06514-8

Cited by 17 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing the length of the arachidate molecule (28Å) with the experimentally determined ML thickness values, good agreement can be found for the 2 ML Pb-arachidate film prepared at c D 35 mN m 1 . The other films deposited at lower surface pressures resulted in layers with lower thickness values, although the molecular areas (calculated from the isotherm compression curves) in the corresponding LB films varied only slightly (19.5-21.2Å 2 ) with the surface pressure.…”

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 56%

“…18,19 Taking this into consideration, the tilt angles of the axes of the molecules were also calculated and presented in Table 1 for the various Cd-arachidate films. The tilt of the molecules in the monolayers was ¾20°for LB films prepared with the usually applied surface pressure of 30 mN m 1 . The largest modification in the orientation (39°from the normal) occurred in the 4 ML LB film deposited at c D 25 mN m 1 .…”

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 99%

“…The tilt of the molecules in the monolayers was ¾20°for LB films prepared with the usually applied surface pressure of 30 mN m 1 . The largest modification in the orientation (39°from the normal) occurred in the 4 ML LB film deposited at c D 25 mN m 1 . The tilt angle of the molecules, calculated from the virtual thickness of the layers, shows a strong correlation with surface pressure (Fig.…”

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 99%

“…Langmuir-Blodgett films are frequently studied by various methods such as IR 1 and UV spectroscopy, 2 XPS, 3,4 neutron and x-ray scattering 5,6 and high-performance imaging techniques. 7,8 Another novel method-surface-sensitive positron annihilation spectroscopy-was introduced for the characterization of LB films.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation and characterization of Langmuir–Blodgett‐type arachidate films

Mohai

Kiss

Tóth

et al. 2002

Surface & Interface Analysis

View full text Add to dashboard Cite

Metal arachidate Langmuir-Blodgett (LB) films with Pb2+ and Cd 2+ cations and varying numbers of monomolecular layers (2 and 4 monolayers, ML) were deposited on trimethylsilylated glass substrates. The arachidate salt formation was commenced during the film deposition from a water-based solution. The extent of salt formation and the build-up of the LB layer were evaluated by quantitative XPS.The completeness of the salt formation was assessed by the atomic ratio of the carboxylic (-COO-)-type carbon component with C 1s binding energy at ∼288.5 eV to that of the selected line of the actual cation.The surface coverage of the glass substrate by the LB film was assessed by calculations based on a substrate-overlayer model using the XPS MultiQuant program. According to this model, the attenuation of the Si 2p photoelectron signal from the substrate by the 2 and 4 ML LB films (with 56 and 112Å thickness, respectively) was calculated and compared with the measured intensities, enabling the completeness of the layer to be evaluated. The layer thickness values calculated from the measured intensity of the layer constituents reflected accurately the thickness derived from the geometry of the arachidate molecule, confirming both the adequacy of the model and the accuracy of the measurement.In agreement with earlier results it was confirmed that salt-based LB films are fairly stable under high vacuum conditions, although slow decomposition of lead arachidate films was detected during prolonged x-ray irradiation.

show abstract

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 56%

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 99%

Section: Thickness Of the Pb-and Cd-arachidate Layersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation and characterization of Langmuir–Blodgett‐type arachidate films

Mohai

Kiss

Tóth

et al. 2002

Surface & Interface Analysis

View full text Add to dashboard Cite

show abstract

“…This pre-coating, produced from a monolayer of behenic acid spread onto a NaCl (10 À2 m), MnCl 2 (10 À4 m) subphase, [42] was shown to be essential for the transfer of a glycolipid bilayer with the hydrophilic headgroups pointing to the surface. [43,44] In order to avoid respreading of the second transferred layer during the interfacial crossing, the plate had to be withdrawn from the aqueous subphase through the compressed monolayer, as recommended elsewhere.…”

mentioning

confidence: 99%

Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

et al. 2005

View full text Add to dashboard Cite

This study deals with the kinetics properties of an enzyme immobilised in a defined orientation in a biomimetic environment. For this purpose, acetylcholinesterase (AChE) was captured at the surface of a nanostructured proteo-glycolipidic Langmuir-Blodgett film through specific recognition by a noninhibitor monoclonal antibody (IgG) inserted in a neoglycolipid bilayer. Modelling of this molecular assembly provided a plausible interpretation of the functional orientation of the enzyme. The AChE activity being stable for several weeks, the enzyme kinetics were investigated, and fitted perfectly with heterogeneous biocatalytic behaviour representative of cellular enzymatic catalysis. The AChE-IgG-glycolipid nanostructure was directly interfaced with an efficient optical device. Such an association, leading to an intimate contact between the nanostructure and the biochemical signal transducer, gives direct access to the intrinsic AChE behaviour. This study thus demonstrates the potential for direct investigation of the kinetic behaviour of an immobilised enzyme on a lipid bilayer through an efficient transduction system.

show abstract

Langmuir-Blodgett Technique for Synthesis of Biomimetic Lipid Membranes

Girard-Egrot

Blum²

Fundamental Biomedical Technologies

View full text Add to dashboard Cite

Linked influence of pH and cations on fatty-acid monolayer integrity related to high-quality Langmuir-Blodgett films

Cited by 17 publications

References 16 publications

Preparation and characterization of Langmuir–Blodgett‐type arachidate films

Preparation and characterization of Langmuir–Blodgett‐type arachidate films

Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

Langmuir-Blodgett Technique for Synthesis of Biomimetic Lipid Membranes

Contact Info

Product

Resources

About