1998
DOI: 10.1016/s0032-9592(98)00005-3
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Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake

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Cited by 138 publications
(94 citation statements)
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“…Lipase activity was determined by spectrophotometric method using p-NPP (ρ-nitrophenyl palmitate) at pH 8.0 [34,35]. The coefficient of extinction of p-nitrophenol (p-NPP), 1.5 x 10 4 L/mol/cm, was determined by measuring absorbance at 410 nm after incubation for 15 min with the enzyme.…”
Section: Lipase Assaymentioning
confidence: 99%
“…Lipase activity was determined by spectrophotometric method using p-NPP (ρ-nitrophenyl palmitate) at pH 8.0 [34,35]. The coefficient of extinction of p-nitrophenol (p-NPP), 1.5 x 10 4 L/mol/cm, was determined by measuring absorbance at 410 nm after incubation for 15 min with the enzyme.…”
Section: Lipase Assaymentioning
confidence: 99%
“…Twelve solid substrates were tested in regard to lipase production in Aspergillus niger [67]. The highest lipase production was obtained with wheat bran (198 Ug -1 ) and gingelly oil cake (169 Ug -1 ).…”
Section: Regulation Of Lipase Production Concerning the Medium Composmentioning
confidence: 99%
“…Many substrates such as rice bran [80], coconut oil cake [65], olive cake and sugar cane bagasse [81], gingelly oil cake [67], babassu oil cake [74], wheat bran [82], Jatropha curcas seed cake [83], niger seed oil cake [84], palm kernal cake [84], groundnut oil cake [85] and mustard oil cake [86] have been used successfully for lipase production. The most important process parameters that have been investigated and optimized up to now are the incubation time, inoculum age, inoculum size, initial moisture content, nature and concentration of carbon and nitrogen sources as well as the C/N ratio of the medium [87].…”
Section: Solid Media Solid Support and Ambient Factors Used For Lipamentioning
confidence: 99%
“…Estas variações também dependem das condições do ensaio, como tempo de incubação, pH e temperatura, do método e do substrato utilizados, tornando, muitas vezes, a comparação difí-cil. Por ex., uma cepa de Aspergillus niger quando cultivada em meio contendo torta de gergelim (meio sólido) mostrou maior estabilidade térmica a 60° C (mantém 69% de sua atividade) que quando foi utilizado um meio de cultivo líquido a mesma temperatura (mantém 57%) 52 .…”
Section: Termoestabilidade Das Enzimas E Estabilidade Frente a Difereunclassified