Lipid composition of Collembola and their food resources in deciduous forest stands—Implications for feeding strategies

Rueß, Liliane; Schütz, Kirsten; Migge-Kleian, Sonja; Häggblom, Max M.; Kandeler, Ellen; Scheu, Stefan

doi:10.1016/j.soilbio.2007.03.002

Cited by 79 publications

(47 citation statements)

References 48 publications

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“…Peaks were named by comparing the calibration mixture with Sherlock software (Microbial ID Inc.). Unsaturation index (UI) of PLFAs was calculated as UI ¼ ((C:1 Â 1) þ (C:2 Â 2) þ (C:3 Â 3) þ (C:4 Â 4))/100, where C:1, C:2, C:3 and C:4 represent fatty acid's proportion (%) with 1, 2, 3 and 4 double bonds, respectively) (Ruess et al, 2007).…”

Section: Plfa Assaysmentioning

confidence: 99%

Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution

Zhou

Baidoo

et al. 2013

The ISME Journal

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Desulfovibrio vulgaris Hildenborough strains with significantly increased tolerance to NaCl were obtained via experimental evolution. A NaCl-evolved strain, ES9-11, isolated from a population cultured for 1200 generations in medium amended with 100 mM NaCl, showed better tolerance to NaCl than a control strain, EC3-10, cultured for 1200 generations in parallel but without NaCl amendment in medium. To understand the NaCl adaptation mechanism in ES9-11, we analyzed the transcriptional, metabolite and phospholipid fatty acid (PLFA) profiles of strain ES9-11 with 0, 100-or 250 mM-added NaCl in medium compared with the ancestral strain and EC3-10 as controls. In all the culture conditions, increased expressions of genes involved in amino-acid synthesis and transport, energy production, cation efflux and decreased expression of flagellar assembly genes were detected in ES9-11. Consistently, increased abundances of organic solutes and decreased cell motility were observed in ES9-11. Glutamate appears to be the most important osmoprotectant in D. vulgaris under NaCl stress, whereas, other organic solutes such as glutamine, glycine and glycine betaine might contribute to NaCl tolerance under low NaCl concentration only. Unsaturation indices of PLFA significantly increased in ES9-11. Branched unsaturated PLFAs i17:1 x9c, a17:1 x9c and branched saturated i15:0 might have important roles in maintaining proper membrane fluidity under NaCl stress. Taken together, these data suggest that the accumulation of osmolytes, increased membrane fluidity, decreased cell motility and possibly an increased exclusion of Na þ contribute to increased NaCl tolerance in NaCl-evolved D. vulgaris.

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Section: Plfa Assaysmentioning

confidence: 99%

Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution

Zhou

Baidoo

et al. 2013

The ISME Journal

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“…The category "eutroglophile" is represented in our study by F. candida and H. nitidus, both classified also as hemiedaphic microorganism consumers, and C. denticulata, also classified as an epigeic plant and animal consumer and fungal feeding secondary decomposer (Ruess et al, 2007;Chahartaghia et al, 2005;Potapov et al, 2016 variable mouthparts morphology and trophic niche preferences (Malcicka et al, 2016;Potapov et al, 2016) and results in significant differences in the enzymatic equipment shown in the recent study. Troglobionts P. spelaeus and P. janosik differed in enzymatic activity, which reflects their different food specialization associated with microhabitat selection.…”

Section: Enzymatic Activity Of Collembola and Affinity To Cave Enviromentioning

confidence: 61%

“…Ponge, 2000;Castaño-Meneses et al, 2004;Fiera, 2014), activity of digestive enzymes (Zinkler, 1971;Urbášek & Rusek, 1994;Berg, 2004), lipid composition (Ruess et al, 2007) or stable isotopes (e.g. Ruess et al, 2004;Chahartaghia et al, 2005;Endlweber et al, 2009;Potapov et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The activity of saccharolytic enzymes in Collembola is associated with species affinity for caves

Parimuchová¹,

Šustr²,

Devetter³

et al. 2018

IJS

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Abstract:The activity of enzymes associated with digestion can reflect food availability and feeding preferences of invertebrates in a particular habitat. Caves are mostly nutrient-poor habitats lacking primary production. In the present study the enzymatic activity of cellulases, trehalases and chitinases was measured in eight collembolan species differently associated with the cave environment: the troglobionts (obligate cave species) Pseudacherontides spelaeus and Protaphorura janosik; the eutroglophiles Ceratophysella denticulata, Folsomia candida and Heteromurus nitidus; the subtroglophiles Hypogastrura aequepilosa and Orthonychiurus rectopapillatus; and the trogloxene (not associated with caves) Megaphorura arctica.Qualitative enzymatic patterns and quantitative differences in species activity were considered in terms of the taxonomic, feeding and ecological classifications of Collembola. Activity of the tested enzymes was confirmed in all species. Cellulolytic and chitinolytic activity seemed to play a crucial role for the discrimination of guilds within all categories. An increasing trend of chitinolytic activity was observed in Collembola associated with the subterranean environment and deeper soil layers, while cellulolytic activity decreased towards more adapted cave forms. Variability in enzymatic activity in cave-dwelling species indicated food specialization across sub-and eutroglophiles and troglobionts, respectively. The results of this study point out that enzymatic activity varies between groups of the cave fauna with different degrees of association to subterranean habitats (cave guilds).

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“…The highest proportions of PUFAs were found by Chamberlain and Black (2005) who saponificated their samples at 701C, and in studies that used mild alkaline methanolysis (Dowling et al, 1986) at a temperature of 371C (Bayley et al, 2001;Holmstrup et al, 2002). On the other hand, relatively low proportions of C 20 PUFAs were found by Haubert et al (2004) and Ruess et al (2007), who used a saponification temperature of 1001C. Hence, for a more accurate estimation of changes in C 20 PUFAs, a low saponification temperature is necessary.…”

Section: Maximization Of C 20 Pufasmentioning

confidence: 99%

Maximized PUFA measurements improve insight in changes in fatty acid composition in response to temperature

Dooremalen

Pel

Ellers

2009

Arch Insect Biochem Physiol

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A general mechanism underlying the response of ectotherms to environmental changes often involves changes in fatty acid composition. Theory predicts that a decrease in temperature causes an increase in unsaturation of fatty acids, with an important role for long-chain poly-unsaturated fatty acids (PUFAs). However, PUFAs are particularly unstable and susceptible to peroxidation, hence subtle differences in fatty acid composition can be challenging to detect. We determined the fatty acid composition in springtail (Collembola) in response to two temperatures (51C and 251C). First, we tested different sample preparation methods to maximize PUFAs. Treatments consisted of different solvents for primary lipid extraction, mixing with antioxidant, flushing with inert gas, and using different temperature exposures during saponification. Especially slow saponification at low temperature (90 min at 701C) in combination with replacement of headspace air with nitrogen during saponification and methylation maximized PUFAs for GC analysis. Applying these methods to measure thermal responses in

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Lipid composition of Collembola and their food resources in deciduous forest stands—Implications for feeding strategies

Cited by 79 publications

References 48 publications

Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution

Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution

The activity of saccharolytic enzymes in Collembola is associated with species affinity for caves

Maximized PUFA measurements improve insight in changes in fatty acid composition in response to temperature

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