Lipid droplet mobilization: The different ways to loosen the purse strings

d’Andréa, Sabine

doi:10.1016/j.biochi.2015.07.010

Cited by 54 publications

(28 citation statements)

References 158 publications

(171 reference statements)

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“…When cells are carbon/energy deprived, fatty acids are mobilized from stored neutral lipids38. Whole-cell fatty acid measurements showed that fatty acids C16:0, C18:1 ω9, C18:1 ω7 and C20:4 are present in resting spores of P. brassicae ; and ARA (C20:4) is the single most abundant fatty acid, constituting 36% of the total amount of fatty acids14.…”

Section: Discussionmentioning

confidence: 99%

Integrated omics study of lipid droplets from Plasmodiophora brassicae

Gao

et al. 2016

Sci Rep

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Plasmodiophora brassicae causes clubroot disease in cruciferous. In this report, lipid droplets were observed in the resting spores of P. brassicae. 295 lipid droplet-associated proteins were identified and categorized into nine groups. Transcriptome analysis of these proteins during three different zoosporic stages revealed differences in gene expression pattern. GO enrichment analysis revealed that these proteins associated with lipid droplets were mainly linked to biosynthesis and metabolism. GC-MS analysis revealed that lipid droplets contain seven types of free fatty acids: saturated fatty acids C16:0 and C18:0, and unsaturated fatty acids C18:1Δ9, C18:1Δ11, C18:2, C20:4 and C20:5. P. brassicae accumulated a large amount of triacylglycerols (TAGs). We systematically analyzed the putative proteins involved in TAG biosynthesis and its metabolic pathway. KEGG pathway analysis defined 3390 genes, including 167 genes involved in lipid metabolism. Transcriptome analysis revealed that 162 candidate enzymes involved in lipid metabolism were differential expressed. Our omics studies are the first to investigate the lipid droplet organelles in P. brassicae, providing a reference resource to study protist lipid droplets.

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Section: Discussionmentioning

confidence: 99%

Integrated omics study of lipid droplets from Plasmodiophora brassicae

Gao

et al. 2016

Sci Rep

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“…In Arabidopsis, 16 oleosin proteins have been identified, including five seed-type oleosins, eight anther-type oleosins, and three seed-and-anther type oleosins ( Table 1). The most abundant oleosin in Arabidopsis seeds is oleosin-1 (OLE1), followed by oleosin-2 (OLE1) (D' Andrea, 2016;Shimada et al, 2008). Seed-type oleosins are involved in regulating both OB size and seed germination.…”

Section: Ob Intrinsic Proteinsmentioning

confidence: 99%

New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development

Shao

Liu

et al. 2019

Front. Plant Sci.

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Oil bodies (OBs) are ubiquitous dynamic organelles found in plant seeds. They have attracted increasing attention recently because of their important roles in plant physiology. First, the neutral lipids stored within these organelles serve as an initial, essential source of energy and carbon for seed germination and post-germinative growth of the seedlings. Secondly, they are involved in many other cellular processes such as stress responses, lipid metabolism, organ development, and hormone signaling. The biological functions of seed OBs are dependent on structural proteins, principally oleosins, caleosins, and steroleosins, which are embedded in the OB phospholipid monolayer. Oleosin and caleosin proteins are specific to plants and mainly act as OB structural proteins and are important for the biogenesis, stability, and dynamics of the organelle; whereas steroleosin proteins are also present in mammals and play an important role in steroid hormone metabolism and signaling. Significant progress using new genetic, biochemical, and imaging technologies has uncovered the roles of these proteins. Here, we review recent work on the structural or metabolic roles of these proteins in OB biogenesis, stabilization and degradation, lipid homeostasis and mobilization, hormone signal transduction, stress defenses, and various aspects of plant growth and development.

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“…Among the array of proteins identified in LDs isolated from different cell types and organisms, a specific set of structural proteins predominates. They belong to the oleosin (OLE) family in plant seeds, to the perilipin (PLIN) family in mammals, and to the major lipid droplet protein family in microalgae (Goold et al, 2015;D'Andrea, 2016;Bersuker and Olzmann, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Oleosins, perilipins, and LDAPs may prevent lipolysis by covering the entire LD surface and shielding triacylglycerols from lipases. They can also regulate lipolysis by interacting with cytoplasmic lipases and regulatory proteins, a well-documented role for perilipins but probably not fulfilled by oleosins (D'Andrea, 2016;Sztalryd and Brasaemle, 2017).…”

Section: Introductionmentioning

confidence: 99%