Lipid formation and γ-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil

Tauk-Tornisielo, Sâmia Maria; Arasato, Luciana S.; Almeida, Alex Fernando de; Govone, José Sílvio; Malagutti, Eleni Nadai

doi:10.1590/s1517-83822009000200025

Cited by 22 publications

(8 citation statements)

References 19 publications

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“…This is might be due to availability of MCFA in coconut oil, and therefore it shows that M. circinelloides should assimilate the MCFA from the coconut into their cells. Our results are consistent with previous studies showing that fungal cells produce more fungal biomass and total lipids when grown in medium-chain-containing oils [42][43][44]. Based on our results, the highest biomass and lipid productivities were achieved with coconut oil mixed medium (g/L•d), while the maximum MCFA contents in terms of the percentage of CDW were obtained with palm oil mixed medium ( Figure 4C).…”

Section: Synergistic Effect Of the Culture Conditions On Fungal Biomasupporting

confidence: 92%

“…Thus, to elucidate the effects of the aforementioned oils on MCFAs, we cultured the engineered strain M65-TE-04 in modified K and R medium together with coconut oil (3%), palm oil (3%), glycerol (3%), or sole glucose (for the control experiments). We chose these concentrations based on some of our preliminary work (data not shown), as well as our previous investigation using 3% diverse oils [43]. The cell dry weight (CDW) and TFA contents for strain M65-TE-04 were shown in Figure 4A,B.…”

Section: Effect Of Carbon Sources On Fungal Biomass and Lipid Accumulmentioning

confidence: 99%

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Optimization of Diverse Carbon Sources and Cultivation Conditions for Enhanced Growth and Lipid and Medium-Chain Fatty Acid (MCFA) Production by Mucor circinelloides

et al. 2019

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Section: Synergistic Effect Of the Culture Conditions On Fungal Biomasupporting

confidence: 92%

Section: Effect Of Carbon Sources On Fungal Biomass and Lipid Accumulmentioning

confidence: 99%

Optimization of Diverse Carbon Sources and Cultivation Conditions for Enhanced Growth and Lipid and Medium-Chain Fatty Acid (MCFA) Production by Mucor circinelloides

et al. 2019

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“…2008; Tauk‐Tornisielo et al . ). In contrast, other reports claim the inverted trend; additions of several types of fatty wastes (e.g.…”

Section: Physiology Of Lipid Accumulation Processmentioning

confidence: 97%

Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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The last years there has been a significant rise in the number of publications in the international literature that deal with the production of lipids by microbial sources (the 'single cell oils; SCOs' that are produced by the so-called 'oleaginous' micro-organisms). In the first part of the present review article, a general overview of the oleaginous micro-organisms (mostly yeasts, algae and fungi) and their potential upon the production of SCOs is presented. Thereafter, physiological and kinetic events related with the production of, mostly, yeast and fungal lipids when sugars and related substrates like polysaccharides, glycerol, etc. (the de novo lipid accumulation process) or hydrophobic substrates like oils and fats (the ex novo lipid accumulation process) were employed as microbial carbon sources, are presented and critically discussed. Considerations related with the degradation of storage lipid that had been previously accumulated inside the cells, are also presented. The interplay of the synthesis of yeast and fungal lipids with other intracellular (i.e. endopolysaccharides) or extracellular (i.e. citric acid) secondary metabolites synthesized is also presented. Finally, aspects related with the lipid extraction and lipidome analysis of the oleaginous micro-organisms are presented and critically discussed.

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“…While 96-well plates are primarily used for basic screening (product, substrate utilisation, tolerance, etc. ), the BioScreen 100-well honeycomb plates are used to assess growth characteristics [68][69][70][71], as well as for screening [72,73]. BioScreen readers hold only two plates, restricting simultaneous measurement to 200 wells.…”

Section: Introductionmentioning

confidence: 99%

High throughput, small scale methods to characterise the growth of marine fungi

et al. 2020

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Various marine fungi have been shown to produce interesting, bioactive compounds, but scaling up the production of these compounds can be challenging, particularly because little is generally known about how the producing organisms grow. Here we assessed the suitability of using 100-well BioScreen plates or 96-well plates incubated in a robot hotel to cultivate eight filamentous marine fungi, six sporulating and two non-sporulating, to obtain data on growth and substrate (glucose, xylose, galactose or glycerol) utilisation in a high throughput manner. All eight fungi grew in both cultivation systems, but growth was more variable and with more noise in the data in the Cytomat plate hotel than in the BioScreen. Specific growth rates between 0.01 (no added substrate) and 0.07 h-1 were measured for strains growing in the BioScreen and between 0.01 and 0.27 h-1 for strains in the plate hotel. Three strains, Dendryphiella salina LF304, Penicillium chrysogenum KF657 and Penicillium pinophilum LF458, consistently had higher specific growth rates on glucose and xylose in the plate hotel than in the BioScreen, but otherwise results were similar in the two systems. However, because of the noise in data from the plate hotel, the data obtained from it could only be used to distinguish between substrates which did or did not support growth, whereas data from BioScreen also provided information on substrate preference. Glucose was the preferred substrate for all strains, followed by xylose and galactose. Five strains also grew on glycerol. Therefore it was important to minimise the amount of glycerol introduced with the inoculum to avoid misinterpreting the results for growth on poor substrates. We concluded that both systems could provide physiological data with filamentous fungi, provided sufficient replicates are included in the measurements.

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Lipid formation and γ-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil

Cited by 22 publications

References 19 publications

Optimization of Diverse Carbon Sources and Cultivation Conditions for Enhanced Growth and Lipid and Medium-Chain Fatty Acid (MCFA) Production by Mucor circinelloides

Optimization of Diverse Carbon Sources and Cultivation Conditions for Enhanced Growth and Lipid and Medium-Chain Fatty Acid (MCFA) Production by Mucor circinelloides

Lipids from yeasts and fungi: physiology, production and analytical considerations

High throughput, small scale methods to characterise the growth of marine fungi

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