Lipid Peroxidation Activity in Women with Chronic Viral Hepatitis

Darenskaya, M. A.; Grebenkina, Lyudmila; Sholokhov, L. F.; Rashidova, M. A.; Semenova, Natalya; Колесников, С. И.; Колесникова, Л. И.

doi:10.1016/j.freeradbiomed.2016.10.525

Cited by 15 publications

(13 citation statements)

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“…Oxidative stress, mainly lipid peroxidation (LPO) has been recognised to be involved in the etiology of several liver diseases [29] including viral and non-viral hepatitis [30]. As such, targeting LPO can be a valuable way of managing liver The activity exhibited by the crude methanol extract was however less potent than both its partitioned n-butanol and ethyl acetate fractions while the aqueous residual fraction had no effect.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Tsafack¹,

Bomgning²,

Kühlborn³

et al. 2018

ABC

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Lipid peroxidation plays a pivotal role in the pathogenicity and maintenance of hepatitis. Thus, substances protecting hepatocyte membranes from lipid peroxidation are of great importance in the management of hepatotoxicity and hepatitis. The present work deals with the in vitro hepatoprotective activity of the methanol extract of Desmodium uncinatum, its sub-fractions, the major isolated compounds and some of their semi-synthetic derivatives in order to study structure activity relationships. Using hydrogen peroxide (H 2 O 2)-induced lipid peroxidation of hepatocyte membranes as a model, the hepatoprotective-guided phytochemical survey of the methanol extract of aerial parts of D. uncinatum was carried out by successive column chromatography. One of the most active compounds (Isovitexin) was chemically transformed to yield new semi-synthetic. The identification of isolated and semi-synthetic compounds was performed using NMR techniques, mass spectrometry and by comparison of their data with those reported in the literature. The n-butanol fraction was the most effective (IC 50 : 22.9 µg/mL) compared to the crude methanol extract (IC 50 : 43.6 µg/mL) and other fractions. The n-butanol sub-fractions FA (containing non-phenolic compounds) and FB (mainly containing phenolic compounds) exhibited respective IC 50 of 14.36 and 128.2 µg/ml.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Tsafack¹,

Bomgning²,

Kühlborn³

et al. 2018

ABC

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“…The choice of dose level was determined by previous studies and evidence of the effectiveness of the medicines in these doses. (8,11) On Day 5 of the experiment, the animals were decapitated under anesthesia and blood samples were collected. Blood serum prepared by centrifugation at 2500 rpm for 20 minutes at +4°C was frozen and stored at -20°C until testing.…”

Section: Methodsmentioning

confidence: 99%

“…(5,6) The severity of this kind disease necessitates the development and research of new types of antihypoxic and immunotropic medicines. (7)(8)(9)(10)(11) Krezacin (hydroxyethylammonium methylphenoxyacetate) and Metaprot (ethylthiobenzimidazole hydrobromide monohydrate) were created in Russia in the middle of the last century and proved to have antihypoxic, energy-stabilizing (ergotropic) and immunomodulating properties. (12)(13)(14)(15)(16) However, studies of these medicines regarding bronchopulmonary diseases have not been carried out.…”

Section: Introductionmentioning

confidence: 99%

Cytokine Profile and Its Correction by Immunomodulators in Experimental Bronchopulmonary Inflammation in Rats

Darenskaya¹,

Mokrenko²,

Shabanov³

et al. 2019

IJBM

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The aim of this study was to assess the changes in the levels of the blood serum pro-and anti-inflammatory cytokines in rats with experimental bronchopneumonia and after administration of immunomodulators. Materials and Methods: Research was carried out on mature male Wistar rats (n=111), weighing 220-250g. The bronchopulmonary inflammation in the rats was modeled by administration of 0.1 ml of gum turpentine in the rats' trachea under etherization. All rats were divided into four groups: Group 1 included intact animals (n=18); Group 2 included animals having bronchopneumonia (n=19); Group 3 included animals having bronchopneumonia treated with Krezacin (n=15); Group 4 included animals having bronchopneumonia treated with Metaprot (n=16). Right after the operation and for the next 5 days, a solution of one of the test immunomodulators was injected (once a day) abdominally in the experimental animals: 25mg/kg of Krezacin or 25mg/kg of Metaprot. On Day 5 of the experiment, the animals were decapitated under anesthesia and blood samples were collected. The concentration of cytokines in the blood was determined by the flow immunofluorometry method with the device Bio-RadLaboratories (USA). Results: The administration of Krezacin in animals with bronchopneumonia, in comparison with untreated animals, was accompanied by a statistically significant increase in the levels of IL-1β (by 3.43 times), IL-2 (by 1.95 times), INF-γ (by 3.29 times), and MCP-1 (by 2.07 times) and a decrease in the levels of TNFα (by 1.85 times) and IL-6 (by 2.20 times). Administration of Krezacin increased anti-inflammatory cytokines considerably: IL-4 by 5.22 times and IL-10 by 2.41 times. Analysis of results after administration of Metaprot demonstrates an increase in the levels of IL-1β (by 2.57 times), IL-2 (by 2 times), INF-γ (by 3.21 times), and MCP-1 (by 1.98 times) and a decrease in the levels of TNFα (by 2.17 times) and IL-6 (by 2.5 times), compared to data on untreated animals. Anti-inflammatory cytokines also increased greatly: IL-4 by 5.3 times and IL-10 by 3.71 times. Conclusion: The results obtained show that investigated preparations reduce the severity and intensity of bronchopneumonia in rats. Krezacin and Metaprot demonstrate the properties of true immunomodulators, increasing protective properties of cytokines and providing the adaptive response of the body in severe bronchopulmonary pathology.

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“…(7) Processes of free-radical oxidation connected with phagocytes, producing ROS, fall into unspecific defense mechanisms. (8)(9)(10)(11)(12)(13) This evolved secretory function of phagocytes is necessary to eliminate bacteria. The intensive ROS generation that triggers LPO processes in cell biomembranes takes place under the conditions of oxygen overconsumption in phagocytosis.…”

Section: Introductionmentioning

confidence: 99%

Lipid Peroxidation Activity and Immune Response in Modeling Inflammatory and Degenerative Damage to the Periodontium of Rats

Darenskaya¹,

Grebenkina²,

Mokrenko³

et al. 2019

IJBM

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The aim of this study was to assess changes in the lipid peroxidation activity and immune response in modeling inflammatory and degenerative damage to the soft tissues of the periodontium of Wistar rats. Materials and Methods: The experiments were carried out on male Wistar rats weighing 220-250g. Modeling the inflammatory and degenerative damage to the soft tissues of the periodontium in animals (n=10) of the test group (TG) was carried out using a single dose of a 2% formaldehyde aqueous solution injected into each side of the outer part of the gums (0.15 ml) at the level of the lower molars of anesthetized animals. The control group (CG) of animals (n=10) received an injection of physiological saline solution in the same volume. In 3 days after damage modeling, the animals were killed by decapitation and blood samples were drawn for testing. The intensity of LPO processes in the blood was estimated by content of conjugated dienes and TBA-reactive substances (TBARS). The status of the AOD system was determined by the SOD activity and GSH level. The immune status of the animals was assessed by several indicators as leukocyte migration inhibition test (LMIT), the phagocytic reaction of neutrophils, and oxygen-dependent and oxygen-independent microbicidal activity of neutrophils. Results: The results obtained showed that the modeling of inflammatory and degenerative damage to the periodontium in rats was characterized by significant changes, affecting both the LPO system and the immune response. An LMIT conducted with various mitogens demonstrated the development of immunodeficiency in TG characterized by high activity of the inflammatory process. Immune disorders in animals of TG were also supported by changes in the phagocytosis system. Low levels on the integral NBT test indicate disorders in the state of oxygen-dependent microbicidal systems of phagocytes. The assessment of the activity of oxygen-independent microbicidal systems of phagocytes in LCT also found disorders in the immune status of animals of TG. Conclusion: Inflammatory and degenerative damage of the soft tissues of the periodontium in rats is accompanied by pronounced disorders in both the LPO system and the immune response.

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Lipid Peroxidation Activity in Women with Chronic Viral Hepatitis

Cited by 15 publications

References 0 publications

Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Cytokine Profile and Its Correction by Immunomodulators in Experimental Bronchopulmonary Inflammation in Rats

Lipid Peroxidation Activity and Immune Response in Modeling Inflammatory and Degenerative Damage to the Periodontium of Rats

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Lipid Peroxidation Activity in Women with Chronic Viral Hepatitis

Cited by 15 publications

References 0 publications

Protective Effects of Extracts, Isolated Compounds from &lt;i&gt;Desmodium uncinatum&lt;/i&gt; and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Protective Effects of Extracts, Isolated Compounds from &lt;i&gt;Desmodium uncinatum&lt;/i&gt; and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Cytokine Profile and Its Correction by Immunomodulators in Experimental Bronchopulmonary Inflammation in Rats

Lipid Peroxidation Activity and Immune Response in Modeling Inflammatory and Degenerative Damage to the Periodontium of Rats

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Resources

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Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes

Protective Effects of Extracts, Isolated Compounds from <i>Desmodium uncinatum</i> and Semi-Synthetic Isovitexin Derivatives against Lipid Peroxidation of Hepatocyte’s Membranes