Lipid peroxidation and antioxidant enzymes in synovial fluid of patients with primary and secondary osteoarthritis of the knee joint

Ostałowska, Alina; Birkner, Ewa; Wiecha, M.; Kasperczyk, Sławomir; Kasperczyk, Aleksandra; Kapolka, Danuta; Zoń‐Giebel, Aleksandra

doi:10.1016/j.joca.2005.08.009

Cited by 133 publications

(141 citation statements)

References 38 publications

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“…Chronic joint diseases may deplete anti-oxidant defenses, whereas acute post-traumatic arthritis may upregulate them. 49,52 The samples used in the present study represent an acute PA with inflammatory exudates in the knee joints, especially in HA subgroup.…”

Section: Discussionmentioning

confidence: 99%

Oxidant and anti‐oxidant systems of synovial fluid from patients with knee post‐traumatic arthritis

Ostałowska

Kasperczyk

et al. 2007

Journal Orthopaedic Research

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It has been suggested that patients with knee post-traumatic arthritis (PA), associated or not to haemarthrosis (HA), display altered oxidant and anti-oxidant systems in their synovial fluid. This study aimed to establish whether this is really the case. Synovial fluid samples were obtained by transdermal arthrocentesis from 69 patients with PA (36 of them had HA) and 22 control subjects. The activities of synovial fluid zinc-copper superoxide dismutase (ZnCuSOD) and manganese superoxide dismutase (MnSOD) isoenzymes, catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione-S-transferase (GST) enzymes, and malondialdehyde (MDA) concentration and synovial fluid viscosity were measured in the study groups. Patients with PA had significantly increased activities of all antioxidant enzymes, except CAT, and MDA concentration than did the controls. However, synovial fluid viscosity was found to be decreased in the study group, mainly in the HA subgroup. Results suggest that excessive free radicals production may exist in synovial fluid of PA patients and may contribute to knee joint destruction.

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Section: Discussionmentioning

confidence: 99%

Oxidant and anti‐oxidant systems of synovial fluid from patients with knee post‐traumatic arthritis

Ostałowska

Kasperczyk

et al. 2007

Journal Orthopaedic Research

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“…W patogenezie choroby zwyrodnieniowej od dawna podkreśla się rolę stresu oksydacyjnego, podając kilka przypuszczalnych mechanizmów miejscowego powstawania reaktywnych form tlenu (RFT) [4,5,6,7]. Poszczególne struktury stawu są chronione przed uszkodzeniami oksydacyjnymi przez wyspecjalizowane enzymy o aktywności antyoksydacyjnej, do których zalicza się dysmutazę ponadtlenkową (SOD), katalazę (CAT) i enzymy związane z przemianami glutationu oraz nieenzymatyczne antyoksydanty [8,9,10]. Leczenie pacjentów z gonartrozą w dużej mierze zależy nie tyle od stopnia zaawansowania choroby zwyrodnieniowej, określanej na podstawie badań radiologicznych, co od wpływu schorzenia na codzienne życie chorych.…”

Section: W St ę Punclassified

Viscosupplementation for the treatment of osteoarthritis of the knee

Ostałowska¹,

Skowron²,

Birkner³

et al. 2017

Ann. Acad. Med. Siles.

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W S T Ę P:Choroba zwyrodnieniowa stawów kolanowych (gonartroza -GA) należy do najczęstszych schorzeń narządu ruchu, a ból i ograniczenie ruchomości kolana są najdotkliwiej odbieranymi przez pacjentów objawami zmian zwyrodnieniowych. Celem pracy było sprawdzenie, czy podanie dostawowe preparatu kwasu hialuronowego wpływa na funkcje stawu kolanowego, wybrane parametry układu antyoksydacyjnego i natężenie stresu oksydacyjnego we krwi u pacjentów z gonartrozą. M A T E R I A Ł I M E T O D Y:Grupa badana 1K obejmowała 96 pacjentów z chorobą zwyrodnieniową stawu kolanowego, którym podano kwas hialuronowy do jednego stawu kolanowego, natomiast do grupy badanej 2K włączono 33 pacjentów, którym podano kwas hialuronowy do obu stawów kolanowych. Badanie prowadzono przez 40 tygodni według ustalonego protokołu. Oznaczono całkowity status oksydacyjny (TOS) osocza, zawartość grup sulfhydrylowych (SH) w surowicy, aktywność katalazy (CAT) w erytrocytach, aktywność dysmutazy ponadtlenkowej (SOD) w osoczu i erytrocytach, aktywność peroksydazy glutationowej (GPx) w erytrocytach. W Y N I K I: Po leczeniu wiskosuplementacyjnym odnotowano zmniejszenie nasilenia bólu oraz poprawę w badanych skalach VAS i HHS, przy czym nieco większą poprawę stwierdzono w przypadku zajęcia jednego kolana. Po leczeniu dostawowym kwasem hialuronowym stwierdzono znamienny spadek aktywności SOD oraz CAT a wzrost aktywności GPx, wzrost stężenia grup SH w obu grupach oraz spadek stężenia TOS. WNIOSKI: Wiskosuplementacja w chorobie zwyrodnieniowej stawu kolanowego istotnie redukuje ból kolana i poprawia jego funkcje oraz wywołuje korzystne zmiany w układzie antyoksydacyjnym krwi. Efekt leczenia jest porównywalny zarówno w przypadku podawania preparatu kwasu hialuronowego do jednego, jaki i do obu stawów kolanowych. S Ł O W A K L U C Z O W Echoroba zwyrodnieniowa stawu kolanowego, kwas hialuronowy, reaktywne formy tlenu, enzymy antyoksydacyjne

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“…As inconsistências são frequentemente atribuídas aos diferentes estágios das afecções articulares no momento da coleta de material para análise (DIMOCK; SICILIANO; MCILWRAITH, 2000) e à diferença de idade dos indivíduos observados. A atividade reduzida da SOD no LS foi atribuída à manifestações mais tardias da OA por Güven et al (2007) e Regan, Bowler e Crapo (2008, enquanto Ostalowska et al (2006) e Ostalowska et al (2007, atribuíram as atividades aumentadas da SOD e da GPx ao caráter agudo da enfermidade articular estudada. Em teoria, a evolução prolongada das enfermidades articulares consome os sistemas de defesa antioxidante, enquanto o processo inflamatório agudo tende a estimulá-los.…”

Section: Análiseunclassified

“…Em teoria, a evolução prolongada das enfermidades articulares consome os sistemas de defesa antioxidante, enquanto o processo inflamatório agudo tende a estimulá-los. Ainda, a correlação negativa entre a atividade das enzimas antioxidantes no LS e a duração da doença articular sugere que, com o curso prolongado da enfermidade, a indução dos sistemas antioxidantes, e consequentemente a atividade das enzimas, diminua progressivamente (OSTALOWSKA et al, 2006). No presente estudo, houve considerável variação na idade com a qual os equinos acometidos por OA (2 a 14 anos) foram admitidos para tratamento.…”

Section: Análiseunclassified

“…Os resultados são conflitantes, tanto nas análises empregando LS, como naquelas realizadas em outros substratos. As inconsistências são frequentemente atribuídas aos diferentes estágios das afecções articulares no momento da coleta de material para análise prolongado da enfermidade, a indução dos sistemas antioxidantes, e consequentemente a atividade das enzimas, diminua progressivamente (OSTALOWSKA et al, 2006). No presente estudo, houve considerável variação na idade com a qual os equinos acometidos por OA (2 a 14 anos) foram admitidos para tratamento.…”

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Investigação laboratorial dos efeitos antioxidantes do plasma processado autólogo nas principais enfermidades articulares de equinos após tratamento artroscópico

Brossi¹

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Palavras-chave:Equino. Líquido sinovial. Hemoderivado. Estresse oxidativo. Plasma processado autólogo. As a cursorial animal, the horse relies on soundness to fully accomplish daily life activities and survival. Horses' natural ability for movement justifies breeding and training investments, and is explored to its maximum during athletic activities. Articular disorders, such as osteoarthritis and osteochondrosis, with their potential to reduce physical performance, are a therapeutic challenge. Autologous haemoderivates have appeared as safe and accessible options for the management of these conditions, but their widespread use has outpaced scientific evidence regarding mechanisms of action and ideal methods for preparation and administration. The antioxidant properties of autologous processed plasma, previously demonstrated in vitro on stimulated equine synovial fluid cells, posed a question on whether these effects would occur in vivo. Therefore, the present study aimed to verify autologous processed plasma´s effects in osteoarthritic and osteochondritic joints, when administered at the end of the arthroscopic procedure, with particular emphasis on possible antioxidant attributes. The study also aimed to detect evidences of oxidative stress in these disorders, what could further justify its use. For this purpose, synovial fluid was obtained immediately before the beginning of the surgery, and analyzed to yield an oxidant/ antioxidant, physical, cellular, molecular and inflammatory profile of osteoarthritis and osteochondrosis. After the end of the procedure, one group of horses received the intraarticular medication and another group of untreated horses served as controls. Forty-eight hours after surgery synovial fluid was retrieved from operated joints, and a second analysis was performed. Physical and cellular analyses were conducted immediately after sample collection and the supernatant was stored at -80º C for further evaluations. These consisted in catalase, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, vitamin E, protein carbonyl groups, chondroitin sulphate, hyaluronic acid, prostaglandin E 2 , total protein and urea determinations.Osteoarthritic joints revealed greater activation of antioxidant defense systems, enzymatic and non-enzymatic, with greater activity of catalase, and greater concentrations of vitamin E, respectively, when compared to osteochondritic joints. Additionally, these joints had higher total nucleated cell counts and prostaglandin E 2 and total protein concentrations, revealing a more inflammatory profile when compared to that of osteochondritic joints. Nonetheless, in osteochondritic joints synovial fluid and matrix components degradation was more pronounced, as there were higher concentrations of chondroitin sulphate and hyaluronic acid in synovial fluid obtained from these joints. Results also showed the occurrence of oxidative stress, 48 hours after the arthroscopic procedure, through higher catalase activity in synovial fluid, reflecting activation ...

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Lipid peroxidation and antioxidant enzymes in synovial fluid of patients with primary and secondary osteoarthritis of the knee joint

Abstract: Patients with KOA display abnormal antioxidant status of synovial fluid with increased activities of antioxidant enzymes and decreased synovial fluid viscosity. Furthermore, synovial fluid viscosity, and activity of GR can be used to distinguish the primary from the secondary type of KOA.

Cited by 133 publications

References 38 publications

Oxidant and anti‐oxidant systems of synovial fluid from patients with knee post‐traumatic arthritis

Oxidant and anti‐oxidant systems of synovial fluid from patients with knee post‐traumatic arthritis

Viscosupplementation for the treatment of osteoarthritis of the knee

Investigação laboratorial dos efeitos antioxidantes do plasma processado autólogo nas principais enfermidades articulares de equinos após tratamento artroscópico

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