Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles

Dinischiotu, Anca; Stanca, Loredana; Grădinaru, Daniela; Petrache, S.N.; Radu, Mihaela; Serban, Andreea Iren

doi:10.1007/978-1-62703-475-3_10

Cited by 22 publications

(15 citation statements)

References 33 publications

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“…Lipid peroxidation expressed by malondialdehyde (MDA) level was assessed using the fluorimetric method described by Dinischiotu et al [79]. A volume of 200 µL of cell lysate diluted correspondingly was mixed with 700 µL of 0.1 N HCl and incubated for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Interaction of New-Developed TiO2-Based Photocatalytic Nanoparticles with Pathogenic Microorganisms and Human Dermal and Pulmonary Fibroblasts

Nica

Stan

Popa

et al. 2017

IJMS

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TiO2-based photocatalysts were obtained during previous years in order to limit pollution and to ease human daily living conditions due to their special properties. However, obtaining biocompatible photocatalysts is still a key problem, and the mechanism of their toxicity recently received increased attention. Two types of TiO2 nanoparticles co-doped with 1% of iron and nitrogen (TiO2-1% Fe–N) atoms were synthesized in hydrothermal conditions at pH of 8.5 (HT1) and 5.5 (HT2), and their antimicrobial activity and cytotoxic effects exerted on human pulmonary and dermal fibroblasts were assessed. These particles exhibited significant microbicidal and anti-biofilm activity, suggesting their potential application for microbial decontamination of different environments. In addition, our results demonstrated the biocompatibility of TiO2-1% Fe–N nanoparticles at low doses on lung and dermal cells, which may initiate oxidative stress through dose accumulation. Although no significant changes were observed between the two tested photocatalysts, the biological response was cell type specific and time- and dose-dependent; the lung cells proved to be more sensitive to nanoparticle exposure. Taken together, these experimental data provide useful information for future photocatalytic applications in the industrial, food, pharmaceutical, and medical fields.

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Section: Methodsmentioning

confidence: 99%

Interaction of New-Developed TiO2-Based Photocatalytic Nanoparticles with Pathogenic Microorganisms and Human Dermal and Pulmonary Fibroblasts

Nica

Stan

Popa

et al. 2017

IJMS

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“…The level of MDA, as a marker of lipid peroxidation, was assessed using the method described by Dinischiotu et al [27]. After exposure to DIO-NPs, 200 µL of protein extract was treated with 700 µL 0.1 N HCl and incubated for 20 min at room temperature.…”

Section: Measurement Of Lipid Peroxidation Levelmentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Balaș

Ciobanu

Burtéa

et al. 2017

Metals

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Abstract:We report the synthesis of dextran-coated iron oxide magnetic nanoparticles (DIO-NPs) with spherical shape and uniform size distribution as well as their accumulation and toxic effects on Jurkat cells up to 72 h. The characterization of dextran-coated maghemite nanoparticles was done by X-ray diffraction and dynamic light scattering analyses, transmission electron microscopy imaging, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, magnetic hysteresis, and relaxometry measurements. The quantification of DIO-NPs intracellular uptake showed a progressive accumulation of iron as a function of time and dose accompanied by additional lysosome formation and an increasing darkening exhibited by a magnetic resonance imaging (MRI) scanner. The cytotoxicity assays revealed a decrease of cell viability and a loss of membrane integrity in a time-and dose-dependent manner. Exposure to DIO-NPs determined an increase in reactive oxygen species level up to 72 h. In the first two days of exposure, the level of reduced glutathione decreased and the amount of malondyaldehyde increased, but at the end of the experiment, their concentrations returned to control values. These nanoparticles could be used as contrast agents for MRI but several parameters concerning their interaction with the cells should be taken into consideration for a safe utilization.

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“…Malondialdehyde (MDA) was assessed by a fluorimetric method [35,36] based on the fluorescence analysis Ex/Em 520/549 of the MDA-TBA adducts formed in acidic conditions, at 37°C. A calibration curve with 1,1,3,3-tetramethoxypropane in the range 0.05-5 lM was used to calculate the MDA concentration.…”

Section: Oxidative Stress Markers Analysismentioning

confidence: 99%

Complex responses to Si quantum dots accumulation in carp liver tissue: Beyond oxidative stress

Serban

Stanca

Sima

et al. 2015

Chemico-Biological Interactions

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Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles

Cited by 22 publications

References 33 publications

Interaction of New-Developed TiO2-Based Photocatalytic Nanoparticles with Pathogenic Microorganisms and Human Dermal and Pulmonary Fibroblasts

Interaction of New-Developed TiO2-Based Photocatalytic Nanoparticles with Pathogenic Microorganisms and Human Dermal and Pulmonary Fibroblasts

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Complex responses to Si quantum dots accumulation in carp liver tissue: Beyond oxidative stress

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