Lipid peroxidation in rat lung induced by neuroleptanalgesia and its components

Chinev, S; Bakalova, Rumiana; S, Kovacheva; Ribarov, S. R.

doi:10.1097/00003643-199811000-00010

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…In the current study, we chose to use fentanyl, which may have contributed to decreased surgical stress [Braz et al, 2009]. As far as we know, fentanyl does not have antioxidant [Chinev et al, 1998;Sayin et al, 2002] or mutagenic effects either in vitro or in vivo [Brambilla and Martelli, 2009]. Furthermore, the benzodiazepine midazolam has a lower antioxidant activity than propofol [Tsuchiya et al, 2001], and the other drugs used in this study (e.g., neostigmine and atropine) did not demonstrate any antioxidant [Kang et al, 1998] or genotoxic effects [Braz et al, 2009].…”

Section: Discussionmentioning

confidence: 91%

Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia

Braz

Mazoti

et al. 2011

Environ and Mol Mutagen

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Propofol, which is widely used as an intravenous anesthetic, has a phenolic structure similar to that of α-tocopherol with antioxidant properties that could prevent genotoxicity and cytotoxicity in lymphocytes of anesthetized patients. The aims of this study were to evaluate oxidative DNA damage and apoptosis in lymphocytes and the expression of DNA repair genes in blood cells from patients undergoing elective surgery under anesthesia with propofol. Twenty healthy adults of both genders (18-50 years old) who were scheduled for otorhinological surgery were enrolled in this study. Blood samples were collected before anesthesia induction (T₁-baseline), 120 min after anesthesia induction (T₂), and on the first postoperative day (T₃). Oxidative DNA damage in peripheral lymphocytes was assessed using the comet assay. Lymphocytes were phenotyped as T helper or cytotoxic T cells, and apoptosis was evaluated using flow cytometry. The expression of DNA repair genes (hOGG1 and XRCC1) was assessed by quantitative polymerase chain reaction. A reduction in the level of oxidized purines in DNA (P < 0.01) was observed 120 min after anesthesia induction, and reduced apoptosis of T helper cells was observed 120 min after anesthesia induction and on the first postoperative day. Down-regulation of hOGG1 and XRCC1 gene expression was observed on the first postoperative day. In conclusion, patients undergoing non-invasive surgery under propofol anesthesia presented lower levels of oxidized purines and apoptosis of T helper lymphocytes. Furthermore, anesthesia with propofol did not directly influence the expression of the DNA repair genes hOGG1 and XRCC1 in blood cells.

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Section: Discussionmentioning

confidence: 91%

Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia

Braz

Mazoti

et al. 2011

Environ and Mol Mutagen

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“…This difference can be attributed to the activities of fentanyl or xylazine on ROS production. Chinev et al (1998) reported that fentanyl and droperidol did not induce lipid peroxidation in rat lungs when used separately or in combination with nitrous oxide. But, injection of fentanyl or droperidol before nitrous oxide significantly reduced prooxidant action of nitrous oxide and was able to inhibit the Fe +2 -catalysed lipid peroxidation in the lung of rats.…”

Section: Discussionmentioning

confidence: 97%

Comparison between Xylazine-Tiletamine-Zolazepam and Fentanyl-Tiletamine-Zolazepam Anaesthetic Combinations on Plasma Oxidative Status in Sheep

Aydılek

2007

Acta Vet. Brno

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It is important to determine varying effects of anaesthetics agents. One of the adverse effects of general anaesthetics are the exogenous sources of reactive oxygen species. In this study, the effects of xylazine-tiletamine-zolazepam (XTZ) and fentanyl-tiletamine-zolazepam (FTZ) combinations on plasma oxidant-antioxidant indicators were compared in sheep. Ten ewes received two different anaesthetic combinations in a ten-day interval. XTZ group was injected with xylazine and tiletamine-zolazepam. FTZ group was injected with fentanyl and tiletamine-zolazepam. Blood samples were collected before and at the 30, 60, 120 min, 24 h and 3 days after anaesthesia. Both anaesthetic regimens caused an increase in malondialdehyde (MDA) concentrations in plasma. The MDA concentrations at 60, 120 min and 24 h in the XTZ group were significantly higher than in the FTZ group. Glutathione (GSH) concentration at 30, 60, 120 min and 24 h was significantly lower than baseline values in XTZ group, while there was only a decrease at 120 min in the FTZ group. GSH concentrations in the XTZ group were significantly lower at 60 and 120 min compared with the FTZ group. Beta carotene concentration was decreased at 120 min and 24 h in the XTZ group, while it decreased only at 120 min in the FTZ group. Glutathione peroxidase (GSH-Px) and catalase (CAT) activities decreased at 120 min and 24 h only in the XTZ group. There were no significant differences in -carotene concentration, activities CAT and GSH-Px activities between groups. In conclusion, both XTZ and FTZ anaesthetic regimens induced oxidative stress in sheep. XTZ combination has more detrimental effect than FTZ combinations on particularly MDA and GSH concentrations. Therefore, FTZ anaesthetic combination is considered to be more suitable for sheep anaesthesia, due to less deteriorating effects on oxidant/antioxidant balance. Anaesthesia, oxidative status, sheepGeneral anaesthesia is commonly used for surgical manipulations in small ruminants. An anaesthetic regimen with minimal side effects is desirable when anaesthesia is required for longer than 1 h in sheep (Lagutchik et al. 1991). One of the undesirable complications of general anaesthetics can be induced by reactive oxygen species (ROS) that occur during anaesthesia (Godin and Garnett 1994). Reactive oxygen species are produced as by-products of metabolism. In organisms, enzymatic (superoxide dismutase, catalase, glutathione peroxidase) and non-enzymatic (urate, cysteine, vitamin E, vitamin C, β-carotene) antioxidant defence systems maintain ROS concentrations in the physiological range. Oxidative stress occurs when the balance between ROS and antioxidants defence is disturbed. ROS cause cellular destruction by oxidative damage of DNA, proteins, lipids and carbohydrates, by changing the structure and function of key cellular constituents that result in mutation, cell damage and death (Simeonova et al. 2004). Thus, ROS play an important role in the pathogenesis of different diseases such as inflammation, carcinogenesis and isch...

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“…donors, and those that produce ROS indirectly. Narcotic drugs and anesthetizing gases are considered major contributors to the production of ROS (43). A large variety of xenobiotics (eg, toxins, pesticides, and herbicides such as paraquat) and chemicals (eg, mustard gas, alcohol) (59,122,184,265) produce ROS as a by-product of their metabolism in vivo.…”

Section: Sources Of Rosmentioning

confidence: 99%

Invited Review: Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification

2002

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Reactive oxygen species (ROS) and other radicals are involved in a variety of biological phenomena , such as mutation, carcinogenesis , degenerative and other diseases, in ammation, aging, and development . ROS are well recognized for playing a dual role as deleterious and bene cial species. The objectives of this review are to describe oxidative stress phenomena , terminology, de nitions, and basic chemical characteristics of the species involved; examine the biological targets susceptible to oxidation and the defense mechanisms of the organism against these reactive metabolites; and analyze methodologies , including immunohistochemica l markers, used in toxicological pathology in the visualization of oxidative stress phenomena . Direct detection of ROS and other free radicals is dif cult, because these molecules are short-lived and highly reactive in a nonspeci c manner. Ongoing oxidative damage is, thus, generally analyzed by measuremen t of secondary products including derivatives of amino acids, nuclei acids, and lipid peroxidation . Attention has been focused on electrochemica l methods based on voltammetry measurements for evaluating the total reducing power of biological uids and tissues. This approach can function as a tool to assess the antioxidant-reducin g pro le of a biological site and follow changes in pathological situations. This review thus includes different topics essential for understanding oxidative stress phenomen a and provides tools for those intending to conduct study and research in this eld.

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Lipid peroxidation in rat lung induced by neuroleptanalgesia and its components

Cited by 9 publications

References 11 publications

Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia

Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia

Comparison between Xylazine-Tiletamine-Zolazepam and Fentanyl-Tiletamine-Zolazepam Anaesthetic Combinations on Plasma Oxidative Status in Sheep

Invited Review: Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification

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