Lipid spectrum in patients with pulmonary tuberculosis

Abstract

The evaluation of the lipid spectrum of the blood plasma and immunocyte membranes is important for timely diagnostic and correction of pathological changes revealed at the cytochemical level. The identification of lipid fractions using high-performance liquid chromatography (HPLC) allows for separate detection of minor fractions of lysophospholipids (lysophosphatidylcholine and lysophosphatidylethanolamine). The study was performed using a photometric sensor and reverse-phase column C18. The following fractions were obtained: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine. The ratio of these fractions is a sensitive marker of pathological shifts in the lipid spectrum both in the blood plasma and immunocyte membranes