Lipidomic Analysis of Bacteria by Thin-Layer Chromatography and Liquid Chromatography/Mass Spectrometry

Goldfine, Howard; Guan, Ziqiang

doi:10.1007/8623_2015_56

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…Methods for LC/ESI–MS/MS have been described [3,15]. Briefly, normal phase LC was performed on an Agilent 1200 Quaternary LC system equipped with an Ascentis Silica HPLC column, 5 µm, 25 cm × 2.1 mm (Sigma-Aldrich, St. Louis, MO) as described.…”

Section: Methodsmentioning

confidence: 99%

The cellular lipids of Romboutsia

Guan

Chen

Gerritsen

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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We have examined the lipids of three isolates, Romboutsia lituseburensis, Romboutsia ilealis, and Romboutsia sp. strain FRIFI, of the newly described genus Romboutsia by two-dimensional thin-layer chromatography (2D-TLC) and by liquid chromatography/mass spectrometry (LC/MS). We have found three phospholipids, phosphatidylglycerol (PG), cardiolipin and phosphatidic acid in all three species. A fourth phospholipid, lysyl-PG, was found in R. lituseburensis and strain FRIFI. Polyprenyl-phosphates were identified in the lipid extracts of all three species. Three glycolipids, mono-, di- and tri-hexosyldiacylglycerol, were common to all three species. An additional glycolipid, tetrahexosyl-diacylglycerol was identified in strain FRIFI. Acylated trihexosyldiacylglycerol and acyl-tetrahexosydiacylglycerol were also found in R. ilealis and strain FRIFI. Remarkably, no alk-1-enyl ether lipids (plasmalogens) were present in Romboutsia as distinct from bacteria of the related genus Clostridium in which these ether lipids are common. We have compared the lipidome of Romboutsia with that recently described for Clostridium difficile, which has plasmalogens, no lysyl-PG, and no tetrahexosyl-diacylglycerol. According to 16S rRNA gene sequencing, Romboutsia spp. and Cosmarium difficile are closely related (>95% sequence identity).

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Section: Methodsmentioning

confidence: 99%

The cellular lipids of Romboutsia

Guan

Chen

Gerritsen

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…The same plates were then sprayed with 0.3% (w/v) molybdenum blue for detection of phospholipids. Finally, the plates were charred for 20 min at 120 C. Glycolipids were detected on separate 2D-TLC plates by spraying with α-naphthol 0.5% in methanol-water 1:1 (v/v) followed by sulfuric acid-water 95:5 (v/v) and heating at 120 C until dark purple color appears (Goldfine and Guan, 2017).…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Lipidomic Analysis of Clostridium cadaveris and Clostridium fallax

Guan

Garrett

Goldfine

2019

Lipids

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The lipidomes of Clostridium fallax and Clostridium cadaveris were studied using thin-layer chromatography (TLC) and normal phase liquid chromatography/mass spectrometry (NPLC/MS). Both species contain diradylglycerol (DRG), monohexosyldiradylglycerol (MHDRG), monohexosyl monoacylglycerol (MHMAG), phosphatidylglycerol (PtdGro), and phosphatidylethanolamine (PtdEtn). DRG, MHDRG, PtdEtn, and PtdGro are present in both diacyl and alk-1-enyl acyl (plasmalogen) forms. Both species contain cardiolipin (Ptd2Gro), which is present in tetraacyl, monoalkenyl-triacyl, and dialkenyl-diacyl forms. Both species contain small amounts of phosphatidylcholine (PtdCho). The presence of octadecadienoic (18:2) acyl chains in some PtdCho species indicates that they arise from the medium because no 18:2 is seen in the other lipids and clostridia generally lack the capacity to synthesize polyunsaturated fatty acids. The major lipidomic differences between these two species are that C. fallax contains a glycerolacetal of plasmenylethanolamine while C. cadaveris contains an ethanolamine-phosphate-modified diacylglycerol. The significance of these lipid compositions is discussed.

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“…Identification of bacterial lipids, particularly in the genus Clostridium, which is an anaerobic bacterium, by LC-MS was described by Goldfine and Guan (2017).…”

Section: Bacteriamentioning

confidence: 99%

Lipidomic Analysis: From Archaea to Mammals

Řezanka

Kolouchová

Gharwalová

et al. 2018

Lipids

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Lipids are among the most important organic compounds found in all living cells, from primitive archaebacteria to flowering plants or mammalian cells. They form part of cell walls and constitute cell storage material. Their biosynthesis and metabolism play key roles in faraway topics such as biofuel production (third-generation biofuels produced by microorganisms, e.g. algae) and human diseases such as adrenoleukodystrophy, Zellweger syndrome, or Refsum disease. Current lipidomic analysis requires fast and accurate processing of samples and especially their characterization. Because the number of possible lipids and, more specifically, molecular species of lipids is of the order of hundreds to thousands, it is necessary to process huge amounts of data in a short time. There are two basic approaches to lipidomic analysis: shotgun and liquid chromatography-mass spectometry. Both methods have their pros and cons. This review deals with lipidomics not according to the type of ionization or the lipid classes analyzed but according to the types of samples (organisms) under study. Thus, it is divided into lipidomic analysis of archaebacteria, bacteria, yeast, fungi, algae, plants, and animals.

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Lipidomic Analysis of Bacteria by Thin-Layer Chromatography and Liquid Chromatography/Mass Spectrometry

Cited by 15 publications

References 23 publications

The cellular lipids of Romboutsia

The cellular lipids of Romboutsia

Lipidomic Analysis of Clostridium cadaveris and Clostridium fallax

Lipidomic Analysis: From Archaea to Mammals

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