Lipopolysaccharide perception in Arabidopsis thaliana: Diverse LPS chemotypes from Burkholderia cepacia, Pseudomonas syringae and Xanthomonas campestris trigger differential defence-related perturbations in the metabolome

Tinte, Morena M.; Steenkamp, Paul A.; Piater, Lizelle A.; Dubery, Ian A.

doi:10.1016/j.plaphy.2020.09.006

Cited by 13 publications

(18 citation statements)

References 43 publications

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“…campestris 8004 ( Xcc ). Advancement in liquid chromatography-mass spectrometry (LC-MS) has improved the use of metabolomics in the study of MAMP-treated plants [ 11 , 12 , 31 ]. Here, chromatographic and spectrometric changes were observed in extracts from both Arabidopsis WT and the mutants after treatments with LPS Pst and LPS Xcc .…”

Section: Discussionmentioning

confidence: 99%

“…These studies highlight the significant role of glucosinolates in plant defence against pathogens. In our group, several glucosinolates including glucohirsutin, glucoerucin, glucobrassicin were putatively annotated from Arabidopsis treated by LPS from B. cepacia [ 11 , 12 ]. The results obtained in this study (both the annotated metabolites, heat map and MetPA analyses) suggested that different LPS chemotypes induced significant accumulation of glucosinolates in the WT compared to the mutants.…”

Section: Discussionmentioning

confidence: 99%

“…As such, this ‘omics’ approach can complement the use of other ‘omics’ technologies since metabolites may not be directly encoded by the genome, and also changes in the transcriptome or proteome do not always correlate with the phenotypes [ 10 ]. Untargeted metabolomics has been used to profile LPS-induced defence-related metabolites such as glucosinolates, flavonoids, hydroxycinnamic acid (HCA) derivatives and lipids in Arabidopsis [ 11 , 12 ]. Moreover, Mareya et al [ 13 ] using liquid chromatography coupled to mass spectrometry (LC-MS), reported defence-related metabolome changes that involved alterations in the levels of phytohormones and several other metabolites in Sorghum bicolor cells treated by the LPS from Burkholderia andropogonis .…”

Section: Introductionmentioning

confidence: 99%

“…campestris 8004 ( Xcc ) (LPS Xcc ) as MAMPs to treat Arabidopsis wild type (WT) and mutants ( lbr2-2 and bak1-4 ) generated by T-DNA insertional mutagenesis. The two LPS chemotypes differ in the chemical ‘molecular patterns’ of the LPS sub-moieties [ 11 , 14 , 15 , 16 ]. Both LPSs from Pst and Xcc have intact O-polysaccharides that characterises a smooth LPS which may differ in molecular structures.…”

Section: Introductionmentioning

confidence: 99%

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Untargeted Metabolomics Profiling of Arabidopsis WT, lbr-2-2 and bak1-4 Mutants Following Treatment with Two LPS Chemotypes

et al. 2022

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Plants perceive pathogenic threats from the environment that have evaded preformed barriers through pattern recognition receptors (PRRs) that recognise microbe-associated molecular patterns (MAMPs). The perception of and triggered defence to lipopolysaccharides (LPSs) as a MAMP is well-studied in mammals, but little is known in plants, including the PRR(s). Understanding LPS-induced secondary metabolites and perturbed metabolic pathways in Arabidopsis will be key to generating disease-resistant plants and improving global plant crop yield. Recently, Arabidopsis LPS-binding protein (LBP) and bactericidal/permeability-increasing protein (BPI)-related proteins (LBP/BPI related-1) and (LBP/BPI related-2) were shown to perceive LPS from Pseudomonas aeruginosa and trigger defence responses. In turn, brassinosteroid insensitive 1 (BRI1)-associated receptor kinase 1 (BAK1) is a well-established co-receptor for several defence-related PRRs in plants. Due to the lack of knowledge pertaining to LPS perception in plants and given the involvement of the afore-mentioned proteins in MAMPs recognition, in this study, Arabidopsis wild type (WT) and mutant (lbr2-2 and bak1-4) plants were pressure-infiltrated with LPSs purified from Pseudomonas syringae pv. tomato DC3000 (Pst) and Xanthomonas campestris pv. campestris 8004 (Xcc). Metabolites were extracted from the leaves at four time points over a 24 h period and analysed by UHPLC-MS, generating distinct metabolite profiles. Data analysed using unsupervised and supervised multivariate data analysis (MVDA) tools generated results that reflected time- and treatment-related variations after both LPS chemotypes treatments. Forty-five significant metabolites were putatively annotated and belong to the following groups: glucosinolates, hydroxycinnamic acid derivatives, flavonoids, lignans, lipids, oxylipins, arabidopsides and phytohormones, while metabolic pathway analysis (MetPA) showed enrichment of flavone and flavanol biosynthesis, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and glucosinolate biosynthesis. Distinct metabolite accumulations depended on the LPS chemotype and the genetic background of the lbr2-2 and bak1-4 mutants. This study highlights the role of LPSs in the reprogramming Arabidopsis metabolism into a defensive state, and the possible role of LBR and BAK1 proteins in LPSs perception and thus plant defence against pathogenic bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Untargeted Metabolomics Profiling of Arabidopsis WT, lbr-2-2 and bak1-4 Mutants Following Treatment with Two LPS Chemotypes

et al. 2022

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“…Lipopolysaccharides (LPS) are microbe-associated molecular patterns (MAMPs) which are present in the outer membrane of gram negative bacteria and can stimulate immunity in a broad range of eukaryotes [ 20 ]. Activation of immune responses by LPS in plants has also been reported [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Pathogens and Elicitors Induce Local and Systemic Changes in Triacylglycerol Metabolism in Roots and in Leaves of Arabidopsis thaliana

Schieferle

Tappe

Korte

et al. 2021

Biology

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Interaction of plants with the environment affects lipid metabolism. Changes in the pattern of phospholipids have been reported in response to abiotic stress, particularly accumulation of triacylglycerols, but less is known about the alteration of lipid metabolism in response to biotic stress and leaves have been more intensively studied than roots. This work investigates the levels of lipids in roots as well as leaves of Arabidopsis thaliana in response to pathogens and elicitor molecules by UPLC-TOF-MS. Triacylglycerol levels increased in roots and systemically in leaves upon treatment of roots with the fungus Verticillium longisporum. Upon spray infection of leaves with the bacterial pathogen Pseudomonas syringae, triacylglycerols accumulated locally in leaves but not in roots. Treatment of roots with a bacterial lipopolysaccharide elicitor induced a strong triacylglycerol accumulation in roots and leaves. Induction of the expression of the bacterial effector AVRRPM1 resulted in a dramatic increase of triacylglycerol levels in leaves, indicating that elicitor molecules are sufficient to induce accumulation of triacylglycerols. These results give insight into local and systemic changes to lipid metabolism in roots and leaves in response to biotic stresses.

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Rootstock rescues watermelon from Fusarium wilt disease by shaping protective root-associated microbiomes and metabolites in continuous cropping soils

Liang

Han

et al. 2022

Plant Soil

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Lipopolysaccharide perception in Arabidopsis thaliana: Diverse LPS chemotypes from Burkholderia cepacia, Pseudomonas syringae and Xanthomonas campestris trigger differential defence-related perturbations in the metabolome

Cited by 13 publications

References 43 publications

Untargeted Metabolomics Profiling of Arabidopsis WT, lbr-2-2 and bak1-4 Mutants Following Treatment with Two LPS Chemotypes

Untargeted Metabolomics Profiling of Arabidopsis WT, lbr-2-2 and bak1-4 Mutants Following Treatment with Two LPS Chemotypes

Pathogens and Elicitors Induce Local and Systemic Changes in Triacylglycerol Metabolism in Roots and in Leaves of Arabidopsis thaliana

Rootstock rescues watermelon from Fusarium wilt disease by shaping protective root-associated microbiomes and metabolites in continuous cropping soils

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