Liposoluble Chemical Constituents in Danshen

Luo, Hou-Wei

doi:10.1007/978-94-017-9469-5_8

Cited by 3 publications

(3 citation statements)

References 58 publications

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“…For zone c (Figure C and Table ), the mass signal at m / z 301.08355 was tentatively assigned , as methylenetanshinquinone (MTQ). Similarity of its structure to TAII supported this hypothesis, as substance c and TAII were located closely to each other on the plate at hR F values 66 and 72, respectively (Figure , track 1).…”

Section: Resultssupporting

confidence: 88%

“…TAII itself is stable, but via exposure to daylight or UV light, it might be changed to oxidation-induced substances. The mechanism of photo-oxidation of TAII was already reported . Hence, the other mass signal at m / z 333.10937 was tentatively assigned as the oxidized molecule of TAII, which might be produced during sample preparation or HPTLC analysis.…”

Section: Resultssupporting

confidence: 88%

“…The still unknown mass signal at m / z 303.09993 was tentatively assigned to be a sodium adduct [M2 + Na] + (Table ). It was supposedly assigned , to be one of the two structural isomers of methylenedihydrotanshinquinone (MDHT) and tetrahydrotanshinone I (THT).…”

Section: Resultsmentioning

confidence: 99%

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From Bioprofiling and Characterization to Bioquantification of Natural Antibiotics by Direct Bioautography Linked to High-Resolution Mass Spectrometry: Exemplarily Shown for Salvia miltiorrhiza Root

Jamshidi-Aidji

Morlock

2016

Anal. Chem.

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Phytochemicals are promising agents in the development of new antibiotics. A streamlined strategy for rapid screening and reliable characterization of antibiotics in botanicals was demonstrated in contrast to the commonly applied chromatographic column fractionation followed by microtiter plate assay. Modern direct bioautography hyphenated to structure elucidation techniques is a straightforward bioanalytical tool, especially if microbiological assays were taken into account. At one go, lipophilic antimicrobials in Salvia miltiorrhiza root samples were analyzed using high-performance thin-layer chromatography (HPTLC) in direct combination with Aliivibrio fischeri and Bacillus subtilis bioassays. The most intense antimicrobials were characterized via HPTLC-high-resolution mass spectrometry. As proof of this streamlined strategy, dihydrotanshinone, cryptotanshinone, and tanshinone IIA were identified and also compared with a reference. Two further antimicrobial zones in the bioautograms were tentatively assigned to be methylenetanshinquinone and tetrahydrotanshinone I (or its structural isomer methylenedihydrotanshinquinone). In another run, a validation study was performed for the bioquantification of ciprofloxacin and marbofloxacin via HPTLC-Bacillus subtilis. This pointed out the improved quality of the performance that was reached. Cryptotanshinone was biologically quantified in two S. miltiorrhiza root samples. Antimicrobials without an available reference standard were calculated as cryptotanshinone-bioequivalents. The results were of relevance, as 1 ng of cryptotanshinone was calculated to be bioequivalent to 0.6 ng and 2 ng of the synthetic antibiotics, ciprofloxacin and marbofloxacin, respectively. For the first time, quantitative direct bioautography via HPTLC-B. subtilis was shown as a reliable tool for streamlined bioprofiling of complex samples.

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Section: Resultssupporting

confidence: 88%

Section: Resultssupporting

confidence: 88%

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From Bioprofiling and Characterization to Bioquantification of Natural Antibiotics by Direct Bioautography Linked to High-Resolution Mass Spectrometry: Exemplarily Shown for Salvia miltiorrhiza Root

Jamshidi-Aidji

Morlock

2016

Anal. Chem.

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Salvia miltiorrhiza: An Economically and Academically Important Medicinal Plant

2019

Compendium of Plant Genomes

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Transcriptomic analysis reveals potential genes involved in tanshinone biosynthesis in Salvia miltiorrhiza

Chang

Wang

et al. 2019

Sci Rep

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Tanshinones are important bioactive components in Salvia miltiorrhiza and mainly accumulate in the periderms of mature roots. Tanshinone biosynthesis is a complicated process, and little is known about the third stage of the pathway. To investigate potential genes that are responsible for tanshinone biosynthesis, we conducted transcriptome profiling analysis of two S. miltiorrhiza cultivars. Differential expression analysis provided 2,149 differentially expressed genes (DEGs) for further analysis. GO and KEGG analysis showed that the DEGs were mainly associated with the biosynthesis of secondary metabolites. Weighted gene coexpression network analysis (WGCNA) was further performed to identify a “cyan” module associated with tanshinone biosynthesis. In this module, 25 cytochromes P450 (CYPs), three 2-oxoglutarate-dependent dioxygenases (2OGDs), one short-chain alcohol dehydrogenases (SDRs) and eight transcription factors were found to be likely involved in tanshinone biosynthesis. Among these CYPs, 14 CYPs have been reported previously, and 11 CYPs were identified in this study. Expression analysis showed that four newly identified CYPs were upregulated upon application of MeJA, suggesting their possible roles in tanshinone biosynthesis. Overall, this study not only identified candidate genes involved in tanshinone biosynthesis but also provided a basis for characterization of genes involved in important active ingredients of other traditional Chinese medicinal plants.

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Liposoluble Chemical Constituents in Danshen

Cited by 3 publications

References 58 publications

From Bioprofiling and Characterization to Bioquantification of Natural Antibiotics by Direct Bioautography Linked to High-Resolution Mass Spectrometry: Exemplarily Shown for Salvia miltiorrhiza Root

From Bioprofiling and Characterization to Bioquantification of Natural Antibiotics by Direct Bioautography Linked to High-Resolution Mass Spectrometry: Exemplarily Shown for Salvia miltiorrhiza Root

Salvia miltiorrhiza: An Economically and Academically Important Medicinal Plant

Transcriptomic analysis reveals potential genes involved in tanshinone biosynthesis in Salvia miltiorrhiza

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