Liposomes: preparation and characterization with a special focus on the application of capillary electrophoresis

Tomnikova, Alice; Orgonikova, Andrea; Křížek, Tomáš

doi:10.1007/s00706-022-02966-0

Cited by 15 publications

(4 citation statements)

References 75 publications

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“…Therefore, a detailed understanding of lipid phase behavior and its implications on liposome functionality is essential for optimizing liposome‐based drug delivery systems, as highlighted by extensive research in this field. [ 34 ]…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, a detailed understanding of lipid phase behavior and its implications on liposome functionality is essential for optimizing liposome-based drug delivery systems, as highlighted by extensive research in this field. [34] Among the various preparation methods employed, the formulations produced by the film hydration and extrusion (FH-E) method exhibited the highest particle size, whereas the formulations produced by the film hydration and sonication (FH-S) method exhibited the lowest particle size. It is probable that this is the case, as the sonication technique employs a higher amount of energy during the liposome formation process.…”

Section: Particle Size Distribution and Zeta Potentialmentioning

confidence: 99%

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Development and in vitro antiviral activity of ivermectin liposomes as a potential drug carrier system

Kocas,

Comoglu,

Ozkul

2024

Archiv der Pharmazie

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This study aimed to assess and compare diverse formulations of ivermectin‐loaded liposomes, employing lipid film hydration and ethanol injection methods. Three lipids (DOPC, SPC, and DSPC) were used in predetermined molar ratios. A total of 18 formulations were created, and a factorial design determined the optimal formulation based on particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency. The average mean particle size, PDI and zeta potential of the selected formulations (F1, F2, F7, F9, and F11) was, respectively, 196.40 ± 44.60 nm, 0.39 ± 0.09, and −40.24 ± 9.17. The encapsulation efficiency exceeded 80%, with a mean loading capacity of 4.00 ± 1.70%. In vitro studies included transmission electron microscopy, Fourier transform infrared spectroscopy, drug release, and antiviral activity assessments against SARS‐CoV‐2. The liposomal formulations demonstrated superior antiviral activity compared to free ivermectin, as indicated by lower IC50 values. The results of this study emphasize the effectiveness of ivermectin‐loaded liposomes in inhibiting viral activity, highlighting their potential as promising candidates for antiviral therapy. The findings suggest that the strategic use of liposomes as drug carriers can significantly modulate and improve the antiviral properties of ivermectin, offering a novel approach to harnessing its full therapeutic potential. Collectively, these results provide a robust foundation for further exploration of ivermectin as a viral protection tool and optimization of its delivery mechanisms.

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Section: Resultsmentioning

confidence: 99%

Section: Particle Size Distribution and Zeta Potentialmentioning

confidence: 99%

Development and in vitro antiviral activity of ivermectin liposomes as a potential drug carrier system

Kocas,

Comoglu,

Ozkul

2024

Archiv der Pharmazie

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“…Intracellular uptake occurs through binding with cell surface receptors or proteins, instigating cell membrane invagination, and forming endocytic vesicles that ferry nanoparticles into cells. Cell membrane fusion entails merging proteins on nanoparticle surfaces with the cell membrane, facilitating direct drug release into cells [23,24]. Osmotic mechanisms enable nanoparticles to traverse cell membranes and enter cells without endocytosis.…”

Section: Mechanisms Of Drug Delivery By Cell Membrane-coated Nanopart...mentioning

confidence: 99%

Advances in the use of cell-membrane encapsulated nanoparticles to target tumor drugs

Feng,

Zheng

2023

BAB

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<p style="text-align: justify;">Cancer treatment faces multiple challenges, including tumor heterogeneity, drug resistance, microenvironment influence, treatment side effects, and treatment cost. The heterogeneity of the tumor makes the effect of the same treatment vary in different patients, so the development of personalized treatment strategies is crucial. In addition, resistance of tumor cells to therapeutic drugs is a major challenge, and new strategies to overcome resistance are needed. As a cutting-edge field of science and technology, nanotechnology has brought great potential and opportunities for tumor treatment. Nanoparticle drug delivery systems improve drug efficacy and reduce side effects through precise targeted delivery and controlled release. Cell-membrane coated nanoparticles show great promise in tumor therapy. Nanoparticles coated with cell membranes have good biocompatibility, can reduce the obstacles of immune rejection and cell uptake, improve the accumulation and retention of drugs in tumor tissues, and have good drug delivery ability, drug stability and control release ability. This review discusses advances in the use of cell-membrane coated nanoparticles to target tumor drugs.</p>

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“…Capillary electrophoresis offers several advantages for the study, characterization, and analysis of liposomes, including low sample consumption, fast and efficient separations, and a high degree of automation [1,2]. Capillary electrophoresis has been used for determination of liposome membrane permeability [3,4], size [5], and charge [6].…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Study on Capillary Surface Modifications for Electrophoretic Separations of Liposomes

Šimonová,

Píplová,

Balouch

et al. 2024

Preprint

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Electroosmotic flow significantly impacts the resolution of separations in capillary electrophoresis and its modification is often necessary. Coating of the inner capillary surface either dynamically or permanently offers a way to alter the electroosmotic flow, potentially reducing the adsorption of analytes, in our case liposomes, to the capillary wall. At first, we measured fluorescently labeled liposomes in an uncoated capillary by capillary electrophoresis with laser-induced fluorescence detection. We used a special procedure of the electrophoretic experiment allowing us to observe the development of peak shape at the early stages of migration. We proved that the liposomes were adsorbed to the capillary wall, which led to a very quick and severe dispersion of their peak during their electromigration. For this reason, we used a commercially coated capillary with polyvinyl alcohol, where at the same separation conditions, we observed the peak of the liposome with a stable shape during the migration. However, this capillary is costly, thus four simple dynamic coating methods were tested for four polymers, namely Pluronic F-127, polyvinyl pyrrolidone K30, polyethylene glycol, and polydiallyldimethylammonium chloride. Among them, we chose a method where we flushed the capillary with a 10% solution of polyvinyl pyrrolidone K30 before the first measurement. In addition, in-house made permanent coating with linear polyacrylamide was investigated, which has led to effective suppression of the electroosmotic flow and stable liposome peak, not dispersed during its migration. Liposome separation using this coating fully confirmed that the studied liposomes are negatively charged and migrate in the anodic direction.

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Liposomes: preparation and characterization with a special focus on the application of capillary electrophoresis

Cited by 15 publications

References 75 publications

Development and in vitro antiviral activity of ivermectin liposomes as a potential drug carrier system

Development and in vitro antiviral activity of ivermectin liposomes as a potential drug carrier system

Advances in the use of cell-membrane encapsulated nanoparticles to target tumor drugs

A Comprehensive Study on Capillary Surface Modifications for Electrophoretic Separations of Liposomes

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