Liquid Chromatographic Analysis of Pentobarbital

A procedure for monitoring m-DET in human urine and serum is described. m-DET is removed from the urine specimen by partitioning into diethyl ether, but solid-phase extraction is used to remove it from human serum. The urine and serum m-DET values are determined by HPLC with a UV detector. The limit of detection was 0.09 micrograms/mL in urine and 0.09 micrograms/g for serum. The percent of m-DET recovered from human urine spiked between 0.50 and 8.00 micrograms/mL was 90 +/- 5.4%. For human serum spiked between 0.25 and 10.00, the percent recovered was 96 +/- 5.9%. The pooled relative standard deviations (RSD) for spiked matrices were 0.06 for urine and 0.06 for serum.

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Liquid Chromatographic Analysis of Pentobarbital

Cited by 3 publications

References 15 publications

Liquid chromatographic determination of barbiturates using a hollow-fibre membrane for postcolumn pH modification

Liquid chromatographic determination of barbiturates using a hollow-fibre membrane for postcolumn pH modification

Determination of Pentobarbital in Biological Samples by Micellar Liquid Chromatography

N,N′Diethyl-m-Toluamide (m-DET): Analysis of an Insect Repellent in Human Urine and Serum by High-Performance Liquid Chromatography

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