Liquid Chromatographic Determination of Minocycline in Human Serum

Leenheer, A.P. De; Nelis, Hans

doi:10.1002/jps.2600681217

Cited by 14 publications

(5 citation statements)

References 28 publications

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“…Neither oxytetracycline nor tetracycline displayed epimerization during evaporation of ethyl acetate-isopropyl alcohol, as was the case with demethylchlortetracycline in our previous doxycycline assay. 10 This method lends itself to routine use. As many as 26-30 analyses were performed daily in connection with a pharflgure 2-Levels of oxytetracycline in plasma of a nonlactating cow after intramuscular administration of a long-acting formulation (20 mglkg).…”

Section: Resultsmentioning

confidence: 99%

Liquid Chromatographic Determination of Oxytetracycline in Bovine Plasma by Double-Phase Extraction

Nelis¹,

Vandenbranden²,

Kruif³

et al. 1992

Journal of Pharmaceutical Sciences

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Section: Resultsmentioning

confidence: 99%

Liquid Chromatographic Determination of Oxytetracycline in Bovine Plasma by Double-Phase Extraction

Nelis¹,

Vandenbranden²,

Kruif³

et al. 1992

Journal of Pharmaceutical Sciences

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“…During the last decade, many studies have dealt with liquid chromatography of the tetracycline group (18)(19)(20)(21). We present the first kinetic study of MC by HPLC over a lengthy period (6 weeks).…”

Section: Discussionmentioning

confidence: 99%

“…MC assays in sebum involved the same device (column, eluent and internal standard). Results were worked out using two wavelength monitoring, i.e., 400 and 352 nm, since the latter significantly decreased the detection limit to 20 nglml (18).…”

Section: Introductionmentioning

confidence: 99%

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Absence of Minocycline in Sebum?

Aubin

Blanc

Guinchard

et al. 1989

The Journal of Dermatology

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The authors studied the bioavailability of minocycline in sebum and serum. Blood and sebum samples were collected weekly for 6 weeks from ten healthy volunteers taking 200 mg of minocycline every day for 4 weeks. Sebum was collected by direct extraction with petroleum ether from the forehead. After evaporation, sebum was weighed on a scale accurate to 10 micrograms. Determination of minocycline in serum and sebum was performed using a high performance liquid chromatography technique (HPLC), with a better detection limit at 352 nm than at 400 nm (20 ng/ml and 0.324 microgram/ml respectively). Our results contrast with other studies since no minocycline was detected in the sebum samples of treated subjects and microbiological assays of minocycline in sebum were also negative. In our opinion, the current hypothesis claiming that the effectiveness of minocycline in the treatment of acne vulgaris is based on sebaceous secretion should be reconsidered.

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“…High-pressure liquid chromatography (HPLC) (3) and polarographic analysis (4) of tetracyclines also have been described, but they require special equipment. Fluorometric analysis is relatively simple and specific for the tetracyclines (5,6).…”

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confidence: 99%