Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains

Mateo, José Juan; Mateo, Rufino; Hinojo, M.J.; Llorens, A.; Jiménez, Misericordia

doi:10.1016/s0021-9673(02)00214-5

Cited by 75 publications

(42 citation statements)

References 35 publications

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“…The principle of solid phase extraction (SPE) columns is a variation of chromatographic techniques that uses a solid phase and a liquid phase to isolate one, or one type, of analyte from a solution. The columns contain different packing materials, ranging from silica gel, C-18 (octadecylsilane), florisil, phenyl, aminopropyl, ion exchange materials, both anionic and cationic, and molecular imprinted polymers (Giraudi et al, 2007;Jornet et al, 2000;Mateo et al, 2002;Vatinno et al, 2008;Yu & Lai, 2010;Zambonin et al, 2001). The generally procedure is to load the sample into column, retain the analyte, wash away impurities, and then elute the analyte.…”

Section: Wwwintechopencommentioning

confidence: 99%

Aflatoxin Measurement and Analysis

Hussain¹

2011

Aflatoxins - Detection, Measurement and Control

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Section: Wwwintechopencommentioning

confidence: 99%

Aflatoxin Measurement and Analysis

Hussain¹

2011

Aflatoxins - Detection, Measurement and Control

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“…In recent decades, several methods have been developed for the determination of DON in cereals such as high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV; Brenn-Struckhofova, Cichna-Markl, Bohm, & Razzazi-Fazeli, 2007), fluorescence (FL) after derivatisation (Mateo, Mateo, Hinojo, Llorens, & Jiménez, 2002;Pussemier et al, 2006), and high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS; Biancardi, Gasparini, Dall'Asta, & Marchelli, 2005). However, demands for rapid screening techniques cannot be fulfilled by these instrumental analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals

Shi

Shen

et al. 2012

Food and Agricultural Immunology

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A rapid and sensitive method based on competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed and validated for the detection of DON (deoxynivalenol) in cereals with the confirmation of the reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). Within this method, the IC50 (half maximal inhibitory concentration of a substance) of the DON-specific monoclonal antibody (MAb) we produced was 61.10 ng/mL. The limit of detection (LOD) value, measured by IC10, was 6.12 ng/ mL, which is below the maximum residue levels (MRLs) established for DON in various cereals. In spiked samples, the recoveries ranged from 79.3% to 115.2% for maize and from 74.3% to 118.3% for wheat. This method was compared with the UPLC-MS/MS method by testing four concentrations. The correlation coefficient for the two methods was 0.9884 in a linear-regression analysis. The results illustrated the reliability of the ciELISA method for the determination of cereal samples.

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“…Although all these methods can successfully determine mycotoxins, issues like false positive results, unfit for quantification of multi-component mycotoxins, and the level of sensitivity still need to overcome. LC/MS/MS has recently attracted increasing attention for the demands of sensitive and selective analyses detection in complex food matrices, biological and environmental sample [11,12].…”

Section: Introductionmentioning

confidence: 99%

Determination of 15 Mycotoxins in Foods and Feeds Using High Performance Liquid Chromatography-Tandem Mass Spectrometry with Gel Permeation Chromatography Combined QuEChERS Purification

Gong¹,

Wang²,

Zhang³

et al. 2012

J Chromat Separation Techniq

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A reliable, sensitive and selective method was successfully developed to determine 15 different mycotoxins simultaneously in foods and feeds. In this method, the homogenized sample was first treated with gel permeation chromatography (GPC) to eliminate most of coextracts, such as pigments, lipids and waxes. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was then carried out as a further cleaned up step, during which the polar interfering compounds such as organic acids and sugars were removed by vortexing with ODS in acetonitrile phase. Using double sample injection method, the analytes were separated by ZORBAX Eclipse plus C18 (1.8 µm, 2.1×100 mm) and detected by a MS/MS system with electrospray ionization (ESI) at multi-reaction monitoring (MRM) mode. The result indicated that the LOD of 15 mycotoxins were ranged from 0.07~5.0 µg/kg. Meanwhile, high correlation coefficients (r 2 >0.996) of 15 mycotoxins were obtained within their respective linear ranges. The average recoveries for lower, intermediate, and high spiked levels ranged from 80.1%~95.5% in company with RSD ranged from 10.5%~19.6%. The method not only represents many advantages including simple pre-treatment, good purification and high sensitivity, but also successfully fit the minimum limiting level requests from various countries including EU, USA and Japan.

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Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains

Cited by 75 publications

References 35 publications

Aflatoxin Measurement and Analysis

Aflatoxin Measurement and Analysis

Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals

Determination of 15 Mycotoxins in Foods and Feeds Using High Performance Liquid Chromatography-Tandem Mass Spectrometry with Gel Permeation Chromatography Combined QuEChERS Purification

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