Liquid chromatography detector based on single and twin electrode thin-layer electrochemistry:  application to the determination of catecholamines in blood plasma

Fenn, R. J.; Siggia, Sidney.; Curran, David J.

doi:10.1021/ac50030a017

Cited by 97 publications

(19 citation statements)

References 23 publications

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“…Dopamine and 5-HT were measured either according to the method of Keller et al (1976) as modified in this laboratory (Lucot et al, 1981), or according to Kotake et al (1985). Norepinephrine was measured using the method of Fenn et al (1978). The brain regions sampled and the amines assayed varied from experiment to experiment (Tables 2-6).…”

Section: " Determination Of Amine Levelsmentioning

confidence: 99%

Variability among brain regions in the specificity of 6-hydroxydopamine (6-OHDA)-induced lesions

Commins

Shaughnessy

Axt

et al. 1989

J. Neural Transmission

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6-Hydroxydopamine (6-OHDA; 200 micrograms, 150 micrograms or 110 micrograms) or vehicle was infused stereotaxically into the lateral ventricles of rats, usually following pretreatment with desmethylimipramine (DMI). Various brain regions were then assayed for dopamine (DA), serotonin (5-HT) and norepinephrine (NE). As expected, 6-OHDA depleted DA in all brain regions examined. Unexpectedly, however, the two highest doses of 6-OHDA significantly decreased 5-HT levels in the hippocampus and increased 5-HT levels in the striatum. In addition, despite pretreatment with doses of DMI commonly considered adequate to block 6-OHDA-induced depletion of NE, all doses of 6-OHDA tested significantly reduced NE levels in the hippocampus, hypothalamus and septum. We interpret our data as suggesting that some brain regions are susceptible to nonspecific toxic effects of 6-OHDA at doses commonly employed. Furthermore, these nonspecific effects may or may not occur, depending on seemingly minor variations in experimental technique.

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Section: " Determination Of Amine Levelsmentioning

confidence: 99%

Variability among brain regions in the specificity of 6-hydroxydopamine (6-OHDA)-induced lesions

Commins

Shaughnessy

Axt

et al. 1989

J. Neural Transmission

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“…LC with electrochemical detection offers a sensitive method for measuring human plasma catecholamines that is simpler than the existing radioenzymatic assays (Hallman et at., 1978;Fenn et at., 1978;Hjemdahl et at., 1979;Fig. 6).…”

Section: Measurement Of Plasma Catecholamines Indoleamines and Drugsmentioning

confidence: 99%

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Adams

Marsden

1982

Handbook of Psychopharmacology

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“…Some electrochemical reactions are chemically irreversible and some are chemically reversible, and therefore, the dual electrode arrangement can eliminate the response to irreversible substances while maintaining the response to reversible substances [6,7] . On the other hand, dual electrodes facing each other is a geometry suitable for enhancing a signal because the electrochemically reversible analyte oxidized at one electrode can be reduced at the other [8]. Since this redox cycling continues, the signal is enhanced.…”

Section: Introductionmentioning

confidence: 99%

Carbon film based ring‐disk and split‐disk dual electrodes as detectors for microbore liquid chromatography

Morita

Solomon

et al. 1996

Electroanalysis

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The electrochemical properties of carbon film based ring-disk and split-disk electrodes in small active volume (0.24-2 pL) radial flow cells were studied in order to employ them in conjunction with microbore liquid chromatography (LC) for determining catechokdmines. The high conversion of dopamine (DA) at the disk of a ring-disk electrode makes the DA limiting current lower than the theoretical value at a low flow rate. The collection efficiency (CE) increases with decreasing flow rate and cell thickness, which is different from previously reported results. A high CE of 0.96 was achieved because of the narrow gap of 5 /Lm.On the other hand, a higher limiting current was observed with the split-disk than with a coinmercially available dual-disk electrode because of its efficient geometry for analyte collection. The cross talk of the DA after reacting at one of the electrode is only 2%. The ringdisk and split-disk electrodes were suitable as series and parallel electrochemical detectors for small bore LC. A detection limit of 103 fg was obtained for DA at the disk of the ring-disk because of the high signal in the thin-layer radial flow cell and the low noise level of the carbon film. In contrast, very slight interference was observed from neighboring electrodes when determining catecholamines at the split-disk.

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Liquid chromatography detector based on single and twin electrode thin-layer electrochemistry: application to the determination of catecholamines in blood plasma

Cited by 97 publications

References 23 publications

Variability among brain regions in the specificity of 6-hydroxydopamine (6-OHDA)-induced lesions

Variability among brain regions in the specificity of 6-hydroxydopamine (6-OHDA)-induced lesions

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Carbon film based ring‐disk and split‐disk dual electrodes as detectors for microbore liquid chromatography

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