Liver surgery Measuring the Rupture Stress Point of Biological Sealant-Collagen Bonding: Validation of a Technique Used after Hepatectomy

Abstract

The aim of this experimental study was to measure the rupture stress point of a fibrin clot situated on a liver, in realistic surgical conditions. The experimental method was carried out with a machined wooden cylinder bonded on the liver, connected with a wire to a setup and pulled at a constant speed, and a sensor was placed on the wire measuring the applied strength. This method, realized in the dog, made it possible to validate a precise and reproducible method designed for testing the adhesive characteristics of biological sealant-collagen bonding on the liver.

Experimental Measure of the Tensile Strength of Biological Sealant-Collagen Association after Hepatectomy in Dogs

“…The adhesive property of the Fibrin clot, which is an essential characteristic of hemostatic seal ants [4], has been measured with the 'rat skin test' developed by Gesting and Lerner [5], However, this test is only suitable for in vitro use. In a previous study [6], we validated an experimental technique which allowed us to measure the rupture stress point of biological sealant-collagen bonding after hepatectomy in realistic surgical conditions. The aim of this study was to report the results of this technique applied after partial hepatectomy in dogs.…”

“…The experimental model (fig. I) has been previously described in detail [6], In brief, the principle of the model was to measure the rupture stress point of a small wooden cylinder bonded on the liver cross-section. Four wooden cylinders with a 1-cm2 cross-section on which a 1-cm2 patch of Colgen® (Immuno.…”