Living and Injectable Porous Hydrogel Microsphere with Paracrine Activity for Cartilage Regeneration

Li, Xing-Chen; Li, Xiaoxiao; Yang, Jian; Lin, Jiawei; Zhu, Yuan; Xu, Xiangyang; Cui, Wenguo

doi:10.1002/smll.202207211

Cited by 59 publications

(34 citation statements)

References 52 publications

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“…In addition, the histological evaluation via HE and SF staining , was performed to assess the cartilage regeneration. As shown in Figure d,e, the articular cartilage in the sham group exhibited a smooth surface, standard structural organization, normal cellularity, and vigorous intensity of SF staining.…”

Section: Resultsmentioning

confidence: 99%

“…Consistently, referring to the International Cartilage Repair Score (ICRS) standard, while the total score is 12, the POSS-PEEP/HA/TGF-β 3 /hMSCs group showed higher ICRS macroscopic scores (11.00 ± 0.58) than the PBS group (4.00 ± 1.00) and the POSS-PEEP/HA/hMSCs group (7.50 ± 1.71, Figure 7b). In addition, the histological evaluation via HE and SF staining 54,55 was performed to assess the cartilage regeneration.…”

Section: Compression Resistance and Hydrogel−tissue Adhesionmentioning

confidence: 99%

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Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration

Cui

Yang

Hong

et al. 2023

ACS Appl. Mater. Interfaces

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The limited self-repair capacity of articular cartilage has motivated the development of stem cell therapy based on artificial scaffolds that mimic the extracellular matrix (ECM) of cartilage tissue. In view of the specificity of articular cartilage, desirable tissue adhesiveness and stable mechanical properties under cyclic mechanical loads are critical for cartilage scaffolds. Herein, we developed an injectable and degradable organic−inorganic hybrid hydrogel as a cartilage scaffold based on polyhedral oligomeric silsesquioxane (POSS)-cored polyphosphate and polysaccharide. Specifically, acrylated 8-arm star-shaped POSS-poly(ethyl ethylene phosphate) (POSS-8PEEP-AC) was synthesized and crosslinked with thiolated hyaluronic acid (HA-SH) to form a degradable POSS-PEEP/HA hydrogel. Incorporation of POSS in the hydrogel increased the mechanical properties. The POSS-PEEP/HA hydrogel showed enzymatic biodegradability and favorable biocompatibility, supporting the growth and differentiation of human mesenchymal stem cells (hMSCs). The chondrogenic differentiation of encapsulated hMSCs was promoted by loading transforming growth factor-β 3 (TGF-β 3 ) in the hydrogel. In addition, the injectable POSS-PEEP/HA hydrogel was capable of adhering to rat cartilage tissue and resisting cyclic compression. Furthermore, in vivo results revealed that the transplanted hMSCs encapsulated in the POSS-PEEP/HA hydrogel scaffold significantly improved cartilage regeneration in rats, while the conjugation of TGF-β 3 achieved a better therapeutic effect. The present work demonstrated the potential of the injectable, biodegradable, and mechanically enhanced POSS-PEEP/HA hybrid hydrogel as a scaffold biomaterial for cartilage regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Compression Resistance and Hydrogel−tissue Adhesionmentioning

confidence: 99%

Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration

Cui

Yang

Hong

et al. 2023

ACS Appl. Mater. Interfaces

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“…Cui et al developed an innovative method to fabricate injectable porous hydrogel microspheres with enduring paracrine activity by incorporating platelet-derived growth factor and exogenous MSCs into gelatin methacrylate hydrogel microspheres (Figure 6A). 57 These microcarriers exhibited exceptional mechanical properties and readily adhered and proliferated on the microspheres, promoting interactions between the extracellular matrix and cells to enhance paracrine effects (Figure 6B). 57 The sustained release of growth factors from the microspheres recruited endogenous MSCs, thereby prolonging the paracrine activity of living microcarriers.…”

Section: Porous Microcarriermentioning

confidence: 99%

“…57 These microcarriers exhibited exceptional mechanical properties and readily adhered and proliferated on the microspheres, promoting interactions between the extracellular matrix and cells to enhance paracrine effects (Figure 6B). 57 The sustained release of growth factors from the microspheres recruited endogenous MSCs, thereby prolonging the paracrine activity of living microcarriers. These microcarriers demonstrated superior secretory properties and anti-inflammatory efficacy, potentially mitigating osteoarthritis progression through the promotion of adherent microenvironment and the F I G U R E 6 (A) Schematic diagram indicating cell migration and chemotaxis measurement.…”

Section: Porous Microcarriermentioning

confidence: 99%

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Fabricating biomimetic materials with ice‐templating for biomedical applications

et al. 2023

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The proper organization of cells and tissues is essential for their functionalization in living organisms. To create materials that mimic natural structures, researchers have developed techniques such as patterning, templating, and printing. Although these techniques own several advantages, these processes still involve complexity, are time‐consuming, and have high cost. To better simulate natural materials with micro/nanostructures that have evolved for millions of years, the use of ice templates has emerged as a promising method for producing biomimetic materials more efficiently. This article explores the historical approaches taken to produce traditional biomimetic structural biomaterials and delves into the principles underlying the ice‐template method and their various applications in the creation of biomimetic materials. It also discusses the most recent biomedical uses of biomimetic materials created via ice templates, including porous microcarriers, tissue engineering scaffolds, and smart materials. Finally, the challenges and potential of current ice‐template technology are analyzed.

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Advanced Hydrogel Material for Meniscus Repair

Li,

Deng,

Yang

et al. 2024

Adv Funct Materials

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The foundation of regenerative medicine is the investigation and progression of hypotheses and approaches for substituting, mending, reconstructing, or regenerating different tissues and organs within the human body. Nevertheless, due to the slender central segment and the entire unattached configuration of the meniscus, the efficacy of conventional therapies is unsatisfactory in reinstating meniscal health and functionality. To overcome these limitations, medical‐type hydrogels have the potential to provide a solution for meniscus repair and regeneration. Their biocompatibility and modifiability enable safe implantations in vivo, effectively modifying the pathophysiology of cells and the surrounding microenvironment at the site of meniscal injury. This article reviews the usage of different implantable medical‐type hydrogels in meniscal regeneration and repair. First, the structure and physiologic function of the meniscus are first briefly described. Following this, the use of varying hydrogels in meniscus repair is highlighted, alongside a discussion of different materials employed in constructing hydrogel implants. Lastly, an analysis of the issues and challenges associated with hydrogels in meniscus repair is presented.

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Living and Injectable Porous Hydrogel Microsphere with Paracrine Activity for Cartilage Regeneration

Cited by 59 publications

References 52 publications

Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration

Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration

Fabricating biomimetic materials with ice‐templating for biomedical applications

Advanced Hydrogel Material for Meniscus Repair

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