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Jacquet, Christophe; Ravelonandro, Michel; Bachelier, J. C.; Dunez, Jean

doi:10.1023/a:1008851821374

Cited by 21 publications

(9 citation statements)

References 45 publications

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“…Deletion of nucleotides coding for the DAG amino acid triplet was achieved by PCR. Production of p∆DAGCP has been described previously (Jacquet et al, 1998). Two other plasmids, in which the nucleotides encoding R##!, Q##" or D#'% were removed, were produced by site-directed mutagenesis as described by Kunkel et al (1987).…”

Section: Methodsmentioning

confidence: 99%

“…The insertion of FLCP and ∆DAGCP genes between a promoter sequence and the NOS terminator has been described previously (Ravelonandro et al, 1992 ;Jacquet et al, 1998). The other modified PPV CP gene (3∆CP) contained deletions of the nucleotides encoding the DAG triplet, R##!, Q##" and D#'%.…”

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

“…Accumulation of FL or modified PPV CP was evaluated by Western blot assay. Pellets from induced or non-induced SG13009 cells were suspended in lysis buffer (Laemmli, 1970 Expression of modified PPV CP genes in plants Expression of modified PPV CP genes in plants (Ravelonandro et al, 1992) (Jacquet et al, 1998) Fig. 1.…”

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

“…Each FL and modified PPV CP coding sequence was inserted into pQE30 downstream of the nucleotides encoding the 6-histidine tag (6-His). Transgenic lines analysed were from the vector-transformed plants BPC (with pBIPCP ; Ravelonandro et al, 1992), 35∆ (with pBI35∆DAGCP ; Jacquet et al, 1998) N. benthamiana leaves were ground in the buffer described by Berger et al (1989) at a dilution of 1 : 4 (w\v). Supernatant (10-30 µl) was electrophoresed on a 12 % polyacrylamide gel (Laemmli, 1970) and blotted onto a nitrocellulose membrane.…”

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

“…The first was to remove the nucleotides coding for the DAG amino acid triplet involved in potyvirus aphid transmission (Atreya et al, 1990(Atreya et al, , 1991Blanc et al, 1997). The new CP construct was designated ∆DAGCP (Jacquet et al, 1998). The second approach was to alter the CP transgene to obtain an encoded CP which would fail to assemble, thereby preventing possible heteroencapsidation in transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

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Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

Jacquet¹,

Delécolle²,

Raccah³

et al. 1998

Journal of General Virology

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Aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (ZYMV-NAT) occurs in transgenic plants expressing the plum pox potyvirus (PPV) coat protein (CP) gene. Heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. In order to prevent this biological risk, several modified PPV CP constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid transmission of heteroencapsidated virions. These constructs were first expressed in Escherichia coli in order to check for the accumulation of

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Section: Methodsmentioning

confidence: 99%

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

Section: Cloning and Expression Of The Different Cp Genes In E Colimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

Jacquet¹,

Delécolle²,

Raccah³

et al. 1998

Journal of General Virology

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Plums

Callahan

2008

Compendium of Transgenic Crop Plants

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Plums arose in three distinct regions, European plums ( Prunus domestica and others) from southern Europe and Asia Minor, Japanese plums ( Prunus salicina and others) from China, and the American plums ( Prunus Americana and others) from North America. The two major commercial species of plum are P. domestica (primarily for the dried fruit industry) and P. salicina (primarily for the fresh fruit industry). Breeding efforts have resulted in many high quality fruit cultivars that have been adapted for various local environmental and disease pressures. At present though, there have been few successes in breeding for resistance to plum pox potyvirus, a virus that not only affects the marketability of the fruit crop but the health of the tree. A number of research groups have begun to develop the techniques necessary to introduce resistance to plum pox virus through genetic engineering. These efforts have resulted in “HoneySweet”, a prune plum ( P. domestica ) that has been found to be highly resistant to plum pox virus in field trials. Work is continuing on improving the techniques used to engineer a plum pox virus‐resistant plum as well as producing more cultivars of plum with the resistance.

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Plum

Esmenjaud¹,

Dirlewanger²

Genome Mapping and Molecular Breeding in Plants

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Cited by 21 publications

References 45 publications

Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

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