Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

Jacquet, Christophe; Delécolle, Brigitte; Raccah, B.; Lecoq, Hervé; Dunez, Jean; Ravelonandro, M.

doi:10.1099/0022-1317-79-6-1509

Cited by 42 publications

(21 citation statements)

References 40 publications

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“…The dendrogram was produced using the neighbour-joining algorithm with 1,000 bootstrap replicates Koonin and Dolja 1993) and the DAG motif at aa 290-292 of the polyprotein (aa 24-26 of CP) is related to the vector transmission of potyviruses (Harrison and Robinson 1988;Atreya et al 1990;Shukla et al 1994). Two highly conserved amino acids, R and D, at aa 436 and 480 of the polyprotein presenting at aa 170 and 214 of CP involve in virion assembly (Jacquet et al 1998). The consensus motifs and amino acids are also present in BaRMV RNA.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids

Zheng

Chen²,

Chen

et al. 2008

Eur J Plant Pathol

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A putative virus-induced disease showing chlorotic spots on leaves of Phalaenopsis orchids was observed in central Taiwan. A virus culture, phalaenopsis isolate 7-2, was isolated from a diseased Phalaenopsis orchid and established in Chenopodium quinoa and Nicotiana benthamiana. The virus reacted with the monoclonal antibody (POTY) against the potyvirus group. Potyvirus-like long flexuous filament particles around 12-15 x 750-800 nm were observed in the crude sap and purified virus preparations, and pinwheel inclusion bodies were observed in the infected cells. The conserved region of the viral RNA was amplified using the degenerate primers for the potyviruses and sequence analysis of the virus isolate 7-2 showed 56.6-63.1% nucleotide and 44.8-65.1% amino acid identities with those of Bean yellow mosaic virus (BYMV), Beet mosaic virus (BtMV), Turnip mosaic virus (TuMV) and Bean common mosaic virus (BCMV). The coat protein (CP) gene of isolate 7-2 was amplified, sequenced and found to have 280 amino acids. A homology search in GenBank indicated that the virus is a potyvirus but no highly homologous sequence was found. The virus was designated as Phalaenopsis chlorotic spot virus (PhCSV) in early 2006. Subsequently, a potyvirus, named Basella rugose mosaic virus isolated from malabar spinach was reported in December 2006. It was found to share 96.8% amino acid identity with the CP of PhCSV. Back-inoculation with the isolated virus was conducted to confirm that PhCSV is the causal agent of chlorotic spot disease of Phalaenopsis orchids in Taiwan. This is the first report of a potyvirus causing a disease on Phalaenopsis orchids

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids

Zheng

Chen²,

Chen

et al. 2008

Eur J Plant Pathol

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“…This motif is also shown to be absent in previously characterized tritimoviruses ONMV, WSMV, BrSMV and WEqMV. However, the two amino acids that are highly conserved among potyvirids in the core of the CP and involved in virion assembly motif [21], Arginine 403 (R) in the core region of the CP and at distance of 44 amino acids aspartic acid 448 (D) at the C terminal was present in the sequence of YOgMV (Fig. 3).…”

Section: Sequence Comparisons and Phylogenetic Relationshipsmentioning

confidence: 97%

Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae

et al. 2009

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Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviridae. The virus was readily mechanically transmitted to its original host plus a narrow host range of monocot species. Serological assays of infected plant extracts using antiserum specific to the closest species in the family Potyviridae were negative. The 3' end of the viral genome was cloned, sequenced and compared to sequences of species in the family Potyviridae. The virus is more closely related to viruses in the genus Tritimovirus than to other genera within the Potyviridae. Based on phylogenetic analyses of the coat protein cistron and flanking genomic regions, we propose this is a distinct viral species of the genus Tritimovirus, tentatively named Yellow oat-grass mosaic virus (YOgMV).

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“…Heteroencapsidation was shown to occur very rarely in potyvirus-resistant transgenic squash and, since it concerns only a single generation (it is a phenotypic change that does not affect the viral genome), heteroencapsidation is considered as having a limited significance in terms of risks (Fuchs & Gonsalves, 2007). In addition, use of constructs with modified CP genes that would prevent heteroencapsidation is possible ( Jacquet et al, 1998).…”

Section: Risk Assessment Studiesmentioning

confidence: 99%

Control of Cucurbit Viruses

Lecoq¹,

Katis

2014

Advances in Virus Research

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Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.

Cited by 42 publications

References 40 publications

Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids

Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids

Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae

Control of Cucurbit Viruses

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