LncRNA‐ATB promotes TGF‐β‐induced glioma cells invasion through NF‐κB and P38/MAPK pathway

Abstract

Glioma constitutes the most aggressive primary intracranial malignancy in adults. We previously showed that long noncoding RNA activated by TGF-β (lncRNA-ATB) promoted the glioma cells invasion. However, whether lncRNA-ATB is involved in TGF-β-mediated invasion of glioma cells remains unknown. In this study, quantitative real-time polymerase chain reaction and western blot analysis were used for detecting the mRNA and protein expression of related genes, respectively. Transwell assay was performed to assess the impact of lncRNA-ATB on TGF-β-induced glioma cells migration and invasion. Immunofluorescence staining was utilized to characterize related protein distribution. Results showed that TGF-β upregulated lncRNA-ATB expression in glioma LN-18 and U251 cells. Overexpression of lncRNA-ATB activated nuclear factor-κB (NF-κB) pathway and promoted P65 translocation into the nucleus, thus facilitated glioma cells invasion stimulated by TGF-β. Similarly, lncRNA-ATB markedly enhanced TGF-β-mediated invasion of glioma cells through activation P38 mitogen-activated protein kinase (P38/MAPK) pathway. Moreover, both the NF-κB selected inhibitor pyrrolidinedithiocarbamate ammonium and P38/MAPK specific inhibitor SB203580 partly reversed lncRNA-ATB induced glioma cells invasion mediated by TGF-β. Collectively, this study revealed that lncRNA-ATB promotes TGFβ-induced glioma cell invasion through NF-κB and P38/MAPK pathway and established a detailed framework for understanding the way how lncRNA-ATB performs its function in TGF-β-mediated glioma invasion.

<p>LncRNA-ATB Promotes the Tumorigenesis of Ovarian Cancer via Targeting miR-204-3p</p>

“…For instance, lncRNA-ATB could promote the proliferation, migration and invasion of cervical cancer or glioma. 13,19 Our current study further confirmed these results, suggesting that lncRNA-ATB could act as an oncogene. In addition, our study firstly found that lncRNA-ATB played a critical role in the tumorigenesis of ovarian cancer, which supplemented the biological function of lncRNA-ATB.…”

“…10 Previous studies have indicated that lncRNA-ATB could significantly promote the progression of multiple malignancies. [11][12][13] However, the role of lncRNA-ATB in the progression of ovarian cancer remains to be explored. Thus, this research aimed to explore the biological function of lncRNA-ATB during the progression of ovarian cancer in vitro and in vivo.…”

lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway

“…Recent studies demonstrated that ATB acts as a competing RNA by binding with microRNA (miR)-494 in lung cancer cells (28), miR-141-3p in gastric cancer cells (29), miR-590-5p in melanoma (30), and miR-200 family members in diverse types of cancer (18,26,31,32). ATB regulates transcriptional coactivator YAP1 (YAP1), NF-κB, p38 mitogen-activated protein kinase (MAPK), Twist, and TGF-β in lung cancer, glioma, breast cancer and malignant melanoma (23,30,33,34). In hepatic fibrosis, ATB knockdown downregulated β-catenin expression by upregulating the expression of endogenous miR-200a (35).…”

Long non-coding RNA ATB promotes human non-small cell lung cancer proliferation and metastasis by suppressing miR-141-3p

“…LncRNA-ATB induced EMT and promoted metastasis of HCC cell lines by sponging miR-200 family [16]. The role of lncRNA-ATB was also investigated in other cancer cell lines, such as prostate cancer [17], glioma [18], cervical cancer [19] and breast cancer [20]. However, the value of lncRNA-ATB in clinical practice of NSCLC is unknown.…”