LncRNA DUXAP8 as a prognostic biomarker for various cancers: A meta-analysis and bioinformatics analysis

Abstract

Background: Dual homeoboxes A pseudogene 8 (DUXAP8) is a newly discovered long noncoding RNA that has been shown to function as an oncogene in a variety of human malignant cancers. By integrating available data, this meta-analysis sought to determine the relationship between clinical prognosis and DUXAP8 expression levels in diverse malignancies.Materials and methods: A systematic search was performed to identify eligible studies from several electronic databases from their inception to 25 October 2021. Pooled odds ratios and hazard ratios with 95% CI were used to estimate the association between DUXAP8 expression and survival. For survival analysis, the Kaplan-Meier method and COX analysis were used. Furthermore, we utilized Spearman’s correlation analysis to explore the correlation between DUXAP8 and tumor mutational burden (TMB), microsatellite instability (MSI), the related genes of mismatch repair (MMR), DNA methyltransferases (DNMTs), and immune checkpoint biomarkers.Results: Our findings indicated that overexpression of DUXAP8 was related to poor overall survival (OS) (HR = 1.63, 95% CI, 1.49–1.77, p < 0.001). In addition, elevated DUXAP8 expression was closely related to poor OS in several cancers in the TCGA database. Moreover, DUXAP8 expression has been associated with TMB, MSI, and MMR in a variety of malignancies.Conclusion: This study revealed that DUXAP8 might serve as a prognostic biomarker and potential therapeutic target for cancer. It can be used to improve cancer diagnosis, discover potential treatment targets, and improve prognosis.

Identification and validation of a ferroptosis-related lncRNA signature to robustly predict the prognosis, immune microenvironment, and immunotherapy efficiency in patients with clear cell renal cell carcinoma

“…LINC01426 modulates CTBP1/miR-423-5p/FOXM1 axis via interacting with IGF2BP1 to aggravate ccRCC progression ( Jiang et al, 2021b ), it also aggravates the malignant progression through miR-661/Mdm2 axis in glioma ( Shu et al, 2022 ), it serves as a prognostic indicator in lung adenocarcinoma by triggering growth and metastasis ( Deng et al, 2022 ), what’s more, in a recently reported study on ferroptosis-related lncRNAs of glioma, LINC01426 was identified to be related to ferroptosis, its knockdown could significantly increase in the Fe 2+ levels and the erastin-induced ROS levels in glioma cells ( Huang et al, 2022 ). LncRNA DUXAP8 acts as an oncogene in most tumors, its abnormal overexpression is associated with the proliferation, invasion, migration, anti-autophagy, and poor prognosis of tumors ( Wang et al, 2022a ; Wang et al, 2022b ), it may act as a potential therapeutic target for cancer ( Wang et al, 2022b ); in a research on identification of necroptosis-related lncRNAs in hepatocellular carcinoma, DUXAP8 was identified as a prognostic lncRNA and related to patients’ prognosis ( Chen et al, 2022b ); In addition to our research, other studies have also found that it is involved in the process of ferroptosis in tumor, such as: it can act with ferroptosis-associated gene FANCD2 to form DUXAP8-miR-29c-FANCD2 axe in hepatocellular carcinoma ( Yang et al, 2022 ), it was also identified as one of ferroptosis-related lncRNAs in kidney carcinoma ( Xing et al, 2021 ). Similar to DUXAP8, lncRNA PVT1 was considered as involved in the ferroptosis progress, it may regulate ferroptosis through miR-214-mediated TFR1 and TP53 expression ( Lu, Xu & Lu, 2020 ), it was identified as one of the ferroptosis-related lncRNAs to construct a panel for predicting tumor progression, microenvironment ( He et al, 2021 ), and radiotherapy response ( Zheng et al, 2021 ) in glioma; in liver cancer, PVT1 is involved in the regulatory mechanism of lncPVT1/miR-214-3p/GPX4 axis and plays a role in ketamine suppressing the viability of liver cancer cells and inducing ferroptosis.…”

The role of piRNAs in predicting and prognosing in cancer: a focus on piRNA-823 (a systematic review and meta-analysis)

“…However, the 5-year survival rate remains relatively low for most cancers. Human health is seriously threatened by cancer [ 56 ]. piRNAs are a class of small non-coding RNA molecules that are expressed in the germline of many species [ 57 ].…”

Prognostic significance of HLA-G in patients with colorectal cancer: a meta-analysis and bioinformatics analysis

“…In addition, there was a research reported that overexpressed miR-365 under hypoxic conditions could target the 3’UTR of the HLA-G mRNA to suppress its expression [ 49 ]. Accumulating evidence has shown that long non-coding RNAs (lncRNAs) could be novel biomarkers to predict the prognosis of patients with cancer [ 50 ]. According to Wang et al’s report [ 51 ], lncRNA myocardial infarction associated transcript (MIAT) was correlated with poor OS, and it was noted that MIAT and miR-133 participated in the proliferation and metastasis of pancreatic cancer [ 52 ].…”