LncRNA LINC00152 promotes laryngeal cancer progression by sponging miR-613

Xiang, Zheng; Dong, Shuying; Sun, Lele; Xu, Jialu; Liu, Jia; Hao, Rui

doi:10.1515/med-2020-0035

Cited by 16 publications

(10 citation statements)

References 37 publications

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“…Accumulating studies have revealed the extensive interactions among lncRNA-miRNA-mRNA regulatory pathways involving ceRNAs, wherein lncRNAs can recover the expression and roles of miRNAs by sequestering miRNAs. [49][50][51] In our study, we used bioinformatics analysis to screen putative miRNAs that may interact with C1QTNF1-AS1. MiR-484 was identified to potentially interact with C1QTNF1-AS1, and this prediction was confirmed using the luciferase reporter and RIP assays.…”

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confidence: 99%

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

Jin¹,

Liu²,

Wang³

et al. 2020

CMAR

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The long noncoding RNA C1QTNF1 antisense RNA 1 (C1QTNF1-AS1) contributes to hepatocellular carcinoma development. However, its expression and roles in colorectal cancer (CRC) have not been fully explored. Therefore, this study determined the expression and roles of C1QTNF1-AS1 in CRC and elucidated its detailed mechanism of action. Methods: C1QTNF1-AS1 expression in CRC tissues and cell lines was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We used Cell Counting Kit-8, flow cytometry, cell migration and invasion assays, and a xenograft tumor model to test the effects of C1QTNF1-AS1 on CRC malignancy. The associations among C1QTNF1-AS1, microRNA-484 (miR-484), and hexokinase 2 (HK2) were explored using luciferase reporter assay, RNA immunoprecipitation, RT-qPCR, and Western blotting. Results: C1QTNF1-AS1 was overexpressed in CRC and related to poor prognosis. C1QTNF1-AS1 interference inhibited CRC cell proliferation, migration, and invasion but induced apoptosis. Furthermore, C1QTNF1-AS1 deficiency impaired tumor growth in vivo. Mechanistically, C1QTNF1-AS1 adsorbed miR-484, thereby increasing the expression of its target HK2. Rescue experiments revealed that the effects of C1QTNF1-AS1 deficiency in CRC cells were reversed by inhibiting miR-484 or upregulating HK2. Conclusion: C1QTNF1-AS1 drives CRC progression by sponging miR-484 and consequently upregulating HK2. The C1QTNF1-AS1/miR-484/HK2 pathway may serve as a diagnostic and therapeutic target for CRC.

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confidence: 99%

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

Jin¹,

Liu²,

Wang³

et al. 2020

CMAR

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“…CYTOR, also known as LINC00152, is a lncRNA with a length of 828 nucleotides and expressed abnormally in many cancers, such as lung cancer, stomach cancer, colon cancer, etc. [15][16][17][18]. It is reported that CYTOR is a key lncRNA in regulating age-related articular cartilage degradation, and CYTOR expression was significantly reduced in the cartilage tissues of OA patients compared to normal samples [9].…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of long non-coding RNA CYTOR induced by icariin promotes the viability and inhibits the apoptosis of chondrocytes

Wang

Zhang

Shen

et al. 2021

BMC Complement Med Ther

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Background Icariin (ICAR) is the main effective component extracted from epimedium, and is reported to have the potential to treat osteoarthritis (OA). However, its pharmacological function on chondrocytes has not been fully clarified. Methods Different doses of ICAR were used to treat chondrocyte cell lines, including CHON-001 and ATDC5. Then the expressions of different lncRNAs were measured by qRT-PCR. Interleukin-1β (IL-1β) was used to simulate the inflammatory response environment of chondrocytes. Overexpression plasmids and short hairpin RNAs of lncRNA CYTOR were used to construct gain-of-function and loss of function models. CCK-8 was conducted to determine the cell viability. Flow cytometry was used to detect the apoptosis of chondrocytes. Enzyme-linked immunosorbent assay (ELISA) was adopted to measure the contents of inflammatory factors (IL-6, IL-8, TNF-α) in the supernatant of the chondrocytes. Results Compared with other lncRNAs, CYTOR was changed most significantly in both CHON-001 and ATDC5 cells after treatment with ICAR. ICAR promotes the viability and inhibits the apoptosis of CHON-001 and ATDC5 cells induced by IL-1β, accompanied with reduced levels of inflammatory factors. Overexpression of CYTOR facilitated the viability of chondrocytes, while repressed their apoptosis and inflammatory response. What’s more, knockdown of CYTOR reversed the protective effects of ICAR on chondrocytes. Conclusion CYTOR was a pivotal lncRNA involved in the protective function of ICAR on chondrocytes.

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“…Among these genes, lncRNA has a crucial role in LSCC progression. For instance, lncRNA FOXD2-AS1 was shown to enhance chemotherapy resistance in LSCC by activating STAT3 [18], and LncRNA LINC00152 can promote laryngeal cancer progression by sponging MiR-613 [19]. However, due to the limited number of screened IALs, many potential and valuable IALs still need to be identi ed to improve the predictive accuracy for LSCC patients.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Immune Associated Long Non-Coding RNAs In Laryngeal Squamous Cell Carcinoma

Liu

et al. 2021

Preprint

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Background: Increasing studies have demonstrated that immune associated lncRNAs (IALs) take an important part in the occurrence and development of multiple cancers. However, the prognosis value of IALs in laryngeal squamous cell carcinoma (LSCC) remains unexplored. This study aimed to evaluate the importance of IALs in LSCC. Methods: RNA sequencing data profiles of LSCC and clinical information of patients were obtained from TCGA dataset. Correlation analysis was performed to screen IALs. Then, a IALs based prognostic signature was constructed through univariate and multivariate Cox regression. The uncover molecular mechanisms of these selected IALs were explored by the bioinformatics analyses.Results: a total of seven differentially expressed survival-associated IALs were found in LSCC patients. a six IALs (LINC02154, SNHG12, CHKB-DT, AL158166.1, AC027307.2 and AL121899.1) based prognostic signature was established, which was a reliable tool to predict the prognosis of LSCC. The area under the curve (AUC) were 0.817 (one-year), 0.847 (three-year) and 0.895 (five-year). Further analysis, there were different infiltration of immune cells between low-risk and high-risk group patients. Additionally, a lncRNA-miRNA-mRNA regulatory network basted on six IALs, 75 miRNAs, and 156 differentially expressed mRNAs was constructed.Conclusions: IALs may play critical role in the occurrence and progression of LSCC, and the IALs based prognostic signature can predict the overall survival rate of LSCC.

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LncRNA LINC00152 promotes laryngeal cancer progression by sponging miR-613

Cited by 16 publications

References 37 publications

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

Up-regulation of long non-coding RNA CYTOR induced by icariin promotes the viability and inhibits the apoptosis of chondrocytes

Prognostic Value of Immune Associated Long Non-Coding RNAs In Laryngeal Squamous Cell Carcinoma

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