LncRNA MIAT Promotes Allergic Inflammation and Symptoms by Targeting MiR-10b-5p in Allergic Rhinitis Mice

Abstract

Background Allergic rhinitis (AR) is one of the most common noninfectious respiratory diseases caused by immunoglobulin E (IgE) response. Objective The study sought to explore the relationship between lncRNA MIAT and miR-10b-5p and their interaction in the regulation of allergic phenotypes in allergic rhinitis (AR) mice. Methods A mice model of AR was constructed using ovalbumin (OVA) sensitization. AR mice were treated with miR-10b-5p agomiR and LNA mediated lncRNA MIAT. The targeting relationship between MIAT and miR-10b-5p was analyzed by the ENCORI website and dual-luciferase reporter assay. The numbers of rubbing and sneezing of mice were counted. Hematoxylin-eosin (HE) staining visualized the eosinophils infiltration in nasal mucosa tissues of mice. The percentage of Th17 cells was quantitated by flow cytometry analysis. ELISA was used to detect the levels of serum OVA-specific IgE, the Th12 cytokine IL-4, and inflammatory cytokines (IL-6, IL-17). Results MIAT was up-regulated in the nasal mucosa of AR mice, while miR-10b-5p was down-regulated. MIAT directly suppressed miR-10b-5p expression in AR mice. The numbers of rubbing and sneezing, the percentage of Th17 cells, and the levels of OVA-specific IgE, IL-4, IL-6, and IL-17 in AR mice were decreased by miR-10b-5p overexpression, which was reversed by MIAT overexpression. The eosinophils infiltration in AR mice was inhibited by miR-10b-5p overexpression, which was also reversed by MIAT overexpression. Conclusion The present study demonstrates that MIAT overexpression Promotes allergic inflammation and symptoms by activating Th17 immune response via miR-10b-5p inhibition.

Noncoding RNAs and Virus and Treatment in Allergic Rhinitis

“…Up-regulated miR-10b-5p 17, IL4, IL6, and IL17 MIAT can promote allergic inflammation and symptoms by activating the th17/ IL4/IL6 immune response via target-inhibited miR-10b-5p in AR patients [47].…”

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

“…CCND2 [814], VCAM1 [615], PDGFB (platelet derived growth factor subunit B) [795], PRKCB (protein kinase C beta) [780], ACTC1 [924], DAPK1 [947], hsa-mir-17-5p [1672], hsa-mir-2110 [1673], TCF3 [1674] and SMARCA4 [1675] are associated with developing cardiovascular diseases. CCND2 [1083], VCAM1 [761], PDGFB (platelet derived growth factor subunit B) [1059], PTCH1 [1098], FOXQ1 [1044], IGF2BP1 [1162], ACTC1 [1192], DAPK1 [947], hsa-mir-2110 [1676], hsa-mir-10b-5p [1677], TCF3 [1678], NR1I2 [1679] and TRIM28 [1680] have been shown to be associated with inflammation. Studies have shown that CCND2 [1495], VCAM1 [1462], PTPRD (protein tyrosine phosphatase receptor type D) [1496], PRKCB (protein kinase C beta) [1476], IGF2BP1 [1545], hsa-mir-200a-3p [1681] and hsa-mir-10b-5p [1682] are an important biomarkers of diabetes mellitus.…”

Roles of long noncoding RNAs in human inflammatory diseases

“…Liu et al also found that lncRNA H19, LINC00895, lnc-SRGAP2C-16, lnc-HLA-C-2, lnc-APOC1-1, and lnc-B3GALT2-1 were associated with the progression of chronic non-atrophic gastritis [ 92 ]. Ma et al revealed that MIAT promoted allergic inflammation in mice with allergic rhinitis [ 93 ]. Liu et al also found that NEAT1 knockdown may attenuate LPS-induced inflammation and apoptosis in HMEECs [ 94 ].…”