LncRNA signature in colorectal cancer

Ghafouri‐Fard, Soudeh; Hussen, Bashdar Mahmud; Gharebaghi, Alireza; Eghtedarian, Reyhane; Taheri, Mohammad

doi:10.1016/j.prp.2021.153432

Cited by 50 publications

(30 citation statements)

References 107 publications

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“…In the epigenetic mechanisms, long non-coding RNAs (lncRNAs) are identified as significant contributors to the initiation, progression and metastasis of CC [ 3 ]. For one of them, LINC01605 was upregulated in 31 out of 38 cases of CC tissues, and its expression in CC cells was higher than that in normal colorectal cells [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancer

et al. 2021

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Background Colorectal cancer (CC) is one of the major contributors to tumor-related death worldwide, and its main cause of death is distant metastasis. Dysregulation of long non-coding RNA (lncRNA) LINC01605 has been implicated in CC. However, its role in metastasis of CC remains elusive. The goal of the study is to uncover the biological function and molecular mechanism of LINC01605 in CC. Methods The differentially expressed lncRNAs were first screened from GSE97300, GSE84983, GSE110715, GSE70880, and GSE75970 microarrays. The correlation between the expression of LINC01605 and the clinical phenotypes of enrolled CC patients (n = 134) was subsequently analyzed. The upstream and downstream regulatory mechanisms of LINC01605 in CC were identified through bioinformatics and RNA-seq analyses. Finally, the effects of related factors on CC cell growth and metastasis were confirmed through functional validation experiments. Results LINC01605, significantly highly expressed in CC, was a prognostic factor for patients with CC. Functional experiments revealed that LINC01605 knockdown inhibited the proliferatory and metastatic potential of CC cells in vitro and in vivo. Moreover, LINC01605 was regulated by SMYD2-EP300-mediated modifications of histone H3K4me3 as well as H3K27ac. LINC01605 was found to bind to METTL3 and promote the m6A modification of SPTBN2 mRNA, thereby facilitating the translation of SPTBN2. Conclusions Overexpression of LINC01605, regulated by SMYD2-EP300-mediated H3K27ac and H3K4me3 modifications, bound to METTL3 protein to promote m6A modification of SPTBN2 mRNA, leading to the development of CC.

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Section: Introductionmentioning

confidence: 99%

LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancer

et al. 2021

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“…CRC prevails as one of the most frequently diagnosed malignancies across the world, accompanied by increasing morbidity and mortality rates [ 13 ]. Meanwhile, the hard-done work of our peers has highlighted that lncRNA SNHG3 functions as a ceRNA to accelerate the progression of CRC [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…On a separate note, long noncoding RNAs (lncRNAs), represent a class of transcripts with over 200 nucleotides that lack apparent protein-coding capacity [ 12 ]. As critical contributors to the epigenetic mechanism, dysregulation of lncRNAs leads to CRC carcinogenesis and malignant transformation [ 13 ]. One such lncRNA, namely small nucleolar RNA host gene 3 (SNHG3), is aberrantly up-regulated in CRC patients, such that SNHG3 up-regulation is associated with advanced clinical stage, distant metastasis, and dismal overall survival [ 3 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Cancer-associated fibroblasts-derived extracellular vesicles carrying lncRNA SNHG3 facilitate colorectal cancer cell proliferation via the miR-34b-5p/HuR/HOXC6 axis

et al. 2022

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Cancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) can mediate tumorigenesis. Long noncoding RNA (LncRNA) SNHG3 is implicated in colorectal cancer (CRC) progression. The current study sought to clarify the role of CAFs-EVs carrying SNHG3 in CRC cell proliferation. Firstly, CAFs and normal fibroblasts (NFs) were cultured and identified, followed by isolation and characterization of CAFs-EVs and NFs-EVs. CRC cells were cultured with CAFs-EVs or CAFs-EVs overexpressing SNHG3. The effects of SNHG3 on CRC cell proliferation was evaluated using CCK-8, colony formation, and EdU staining assays. The binding relationships among SNHG3, miR-34b-5p, and HuR were validated, in addition to analyzing the binding between HuR and HOXC6. Lastly, xenograft tumor model was established to verify the role of CAFs-EVs carrying SNHG3 in vivo. SNHG3 was highly expressed in CRC cells and CAFs-EVs, whereas CAFs-EVs facilitated CRC cell proliferation. Mechanically, CAFs-EVs carried SNHG3 into CRC cells to upregulate HuR expression by competitively binding to miR-34b-5p, promote the binding of HuR and HOXC6, and enhance HOXC6 transcription. miR-34b-5p over-expression or HOXC6 silencing annulled the effect of CAFs-EVs. SNHG3 carried by CAFs-EVs facilitated CRC proliferation via the miR-34b-5p/HuR/HOXC6 axis in vivo. Collectively, our findings indicated that CAFs-EVs carried SNHG3 into CRC cells to upregulate HuR expression by sponging miR-34b-5p and finally enhance HOXC6 transcription, thereby facilitating CRC cell proliferation.

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“… 29 After exploring the lncRNAs in colorectal cancer, HOXA11-AS, MEG3, SLCO4A1-AS1, SPINT1-AS1, and DANCR lncRNAs were discovered strong power for the diagnosis. 30 Four-methylated LncRNAs were considered as biomarkers for predicting the survival of osteosarcoma. 31 The prognosis of diffuse large B‐cell lymphoma might be predicted by a signature based on 6 lncRNAs.…”

Section: Discussionmentioning

confidence: 99%

Nine Pyroptosis-Related lncRNAs are Identified as Biomarkers for Predicting the Prognosis and Immunotherapy of Endometrial Carcinoma

Liang¹,

Hu²,

Tang³

et al. 2021

IJGM

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LncRNA signature in colorectal cancer

Cited by 50 publications

References 107 publications

LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancer

LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancer

Cancer-associated fibroblasts-derived extracellular vesicles carrying lncRNA SNHG3 facilitate colorectal cancer cell proliferation via the miR-34b-5p/HuR/HOXC6 axis

Nine Pyroptosis-Related lncRNAs are Identified as Biomarkers for Predicting the Prognosis and Immunotherapy of Endometrial Carcinoma

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