lncRNA SNHG16 promotes glioma tumorigenicity through miR-373/EGFR axis by activating PI3K/AKT pathway

Zhou, Xiangyang; Liu, Hong; Ding, Zheng-Bin; Xi, Hai-Peng; Wang, Guangwei

doi:10.1016/j.ygeno.2019.06.017

Cited by 57 publications

(42 citation statements)

References 30 publications

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“…22 For example, lncRNA SNHG16 promotes glioma tumorigenicity through sponging miR-373-3p, therefore increasing estimated glomerular filtration rate expressing and subsequently activating phosphatidylinositol 3-kinase/AKT (PI3K/AKT) signaling pathway. 23 Similarly, our work found the direct binding between NEAT1 and miR-92b using RIP and RNA pull-down assay. Meanwhile, the luciferase reporter assay indicated that miR-92b expression was negatively correlated with NEAT1.…”

Section: Discussionsupporting

confidence: 69%

Long Noncoding RNA NEAT1 Suppresses Proliferation and Promotes Apoptosis of Glioma Cells Via Downregulating MiR-92b

Liu

Zou

et al. 2020

Cancer Control

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Background: The mechanisms underlying the proliferation and apoptosis of glioma cells remain unelucidated. A recent study has revealed that microRNA-92b (miR-92b) inhibits apoptosis of glioma cells via downregulating DKK3. Notably, long noncoding RNA nuclear-enriched abundant transcript 1 (NEAT1) is predicted to have a possible interaction with miR-92b. Objective: This study aimed to identify whether NEAT1 affects glioma cell proliferation and apoptosis via regulating miR-92b. Methods: The expression of NEAT1 was compared between glioma tissues and adjacent tissues as well as between glioma cells and normal astrocytes using quantitative real-time polymerase chain reaction. Glioma cell proliferation was determined by using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and glioma cell apoptosis was determined by using the flow cytometry. Results: The expression of NEAT1 was low in glioma tissues and cells compared to the normal ones. Overexpression of NEAT1 inhibited proliferation and promoted apoptosis of glioma cell lines (U-87 MG and U251). The interaction between NEAT1 and miR-92b was confirmed using RNA immunoprecipitation, RNA pull-down assay, and luciferase reporter assay. Importantly, the tumor suppressor function of overexpressing NEAT1 was achieved by downregulating miR-92b and subsequently upregulating DKK3. Conclusion: Our findings indicated that NEAT1 acts as a tumor suppressor in glioma cells, which provides a novel target in overcoming glioma growth.

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Section: Discussionsupporting

confidence: 69%

Long Noncoding RNA NEAT1 Suppresses Proliferation and Promotes Apoptosis of Glioma Cells Via Downregulating MiR-92b

Liu

Zou

et al. 2020

Cancer Control

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“…Recent evidence has indicated that lncRNAs can function as molecular sponges for miRNAs to regulate the The expression of Cleaved-Caspase-3, Cleaved-Caspase-9, Cyclin D1, PI3K, p-AKT, AKT, Foxo3a, Bax, Bcl-2, and total Caspase-3/9 proteins in RPMI-8226 and NCI-H929 cells was measured by western blot at 48 h after transfection with miR-342-3p mimics or miR-NC expression and function of target miRNAs [17]. SNHG16 has been reported to function as a molecular sponge for multiple miRNAs in cancers, such as miR-98-5p [18], miR-135a [8], and miR-373 [19]. We investigated the molecule mechanism of SNHG16 regulation in the progression of MM by using bioinformatics analysis to predict putative binding miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA SNHG16 suppresses multiple myeloma cell proliferation by sponging miR-342-3p

et al. 2020

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Background: Aberrant expression of long non-coding RNAs (lncRNAs) is closely associated with development and prognosis of human cancers. LncRNA SNHG16 is reportedly involved in human cancer; however, its roles in multiple myeloma (MM) remain unclear. Methods: In this study, we investigated the function and molecular mechanisms of SNHG16 in MM. MM cells were transfected with si-SNHG16 or si-NC. SNHG16 expression levels was measured by qRT-PCR. Cell proliferation was monitored using the MTS. Flow cytometry assay was performed to measure the cell cycle and apoptosis. Luciferase reporter assay were performed to confirm the sponged miRNAs of SNHG16. Results: SNHG16 expression was up-regulated in MM tissues. SNHG16 knockdown suppressed cell proliferation, arrested cell cycle transition from G1 to S phase, and promoted the apoptosis of MM cells. Moreover, SNHG16 knockdown promoted cleaved-Caspase-3, cleaved-Caspase-9, Foxa3a, and Bax expression, while markedly inhibiting CCND1, Bcl-2, Cyclin D1, PI3K, and p-AKT expression in MM cells. miR-342-3p was a direct target of SNHG16. SNHG16 knockdown significantly increased miR-342-3p expression in MM cells. Overexpression miR-342-3p markedly suppressed cell proliferation, arrested cell cycle transition from G1 to S phase, and promoted apoptosis of MM cells. Overexpression of miR-342-3p markedly promoted cleaved-Caspase-3/-9, Foxa3a, and Bax expression, and inhibited CCND1, Bcl-2, Cyclin D1, PI3K, and p-AKT expression in MM cells. Additionally, repression of miR-342-3p could rescue the effect of SNHG16 knockdown on MM cell proliferation, cycle arrest, apoptosis, and related protein expression. Conclusion: Knockdown of lncRNA SNHG16 suppresses MM cell proliferation by sponging miR-342-3p, implicating SNHG16 as a novel therapeutic target for MM.

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“…However, its association with miR-373 remains largely unclear. miR-373 plays a role in the ceRNA network in ovarian cancers and gliomas [ 21 , 22 ]. Our luciferase reporter assay and RT-qPCR analysis showed that miR-373, which is poorly expressed in PE, is a target of NEAT1 in trophoblast cells.…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis

Teng

Song³

et al. 2020

Med Sci Monit

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lncRNA SNHG16 promotes glioma tumorigenicity through miR-373/EGFR axis by activating PI3K/AKT pathway

Cited by 57 publications

References 30 publications

Long Noncoding RNA NEAT1 Suppresses Proliferation and Promotes Apoptosis of Glioma Cells Via Downregulating MiR-92b

Long Noncoding RNA NEAT1 Suppresses Proliferation and Promotes Apoptosis of Glioma Cells Via Downregulating MiR-92b

Knockdown of lncRNA SNHG16 suppresses multiple myeloma cell proliferation by sponging miR-342-3p

Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis

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