LncRNA XIST promotes chemoresistance of breast cancer cells to doxorubicin by sponging miR‐200c‐3p to upregulate ANLN

Zhang, Min; Wang, Feng; Xiang, Zhen; Huang, Teng; Zhou, Wei‐Fang

doi:10.1111/1440-1681.13307

Cited by 48 publications

(34 citation statements)

References 24 publications

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“…Interestingly, miR-940 also regulates the in ammatory response in osteoarthritis (OA) (15) and spinal cord injury (16). lncRNA XIST enhances ANLN by up-regulating miR-200c-3p, promotes the chemical resistance of breast cancer cells to doxorubicin and provides a potential new therapeutic target for the breast (17). The exosomes rich in miR-2277-30p tend to have a high abundance of β4 and lead to a cancer promoting effect (18).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of the Regulatory lncRNA-miRNA-mRNA Network in Patients with Neuropathic Pain

Zhang

Liu

Song

et al. 2020

Preprint

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Background: Neuropathic pain (NP) is the main form of chronic pain, caused by damage to the nervous system and dysfunction. Methods: Here, we explore the key molecules involved in the development of NP condition via identification of lncRNA-miRNA-mRNA expression pattern of patients with NP. We identified differentially expressed miRNAs, lncRNA and mRNA through a comprehensive analysis strategy. Subsequently, we used bioinformatics approach to perform pathway enrichment analysis on DEGs and protein-protein interaction analysis. Combined with the three datasets, the lncRNA-miRNA-mRNA network was constructed. It will then be used as targets for drug prediction. Results: The results showed that a total of 8,251 DEGs (4,193 upregulated and 4,058 downregulated) were identified from the three microarray datasets, 959 DEmiRs (455 upregulated and 504 downregulated), 2,848 DElncs (1,324 upregulated and 1,524 downregulated). GO analysis showed that DEGs are mainly enriched in blood circulation, regulation of membrane potential and regulation of ion transmembrane transport. KEGG results showed that DEGs are enriched in neuroactive ligand-receptor interaction, PI3K-Akt signaling pathway and MAPK signaling pathway. When the correlation is set to above 0.8, a total of 31 lncRNAs, 36 miRNAs and 24 mRNAs were screened in the lncRNA-miRNA-mRNAs network. The results of drug prediction indicated the targeted drugs mainly include INDOMETHACIN, GLUTAMIC ACID and PIRACETAM. Conclusion: The lncRNA-miRNA-mRNA network has been carried out a comprehensive biological information analysis and predicted the potential therapeutic application of drugs in patients with NP. The corresponding data has a certain reference for studying the pathological mechanism of NP.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of the Regulatory lncRNA-miRNA-mRNA Network in Patients with Neuropathic Pain

Zhang

Liu

Song

et al. 2020

Preprint

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“…LncRNA can act as a ceRNA by sponging miRNA to reduce the regulatory effect of miRNA on its target [22,25]. To explore the regulatory mechanisms by which lncRNA MPNCR affects the proliferation of BMECs, we determined its subcellular localization using the PCR method.…”

Section: Lncrna Mpncr Acts As a Molecular Sponge For Mir-31mentioning

confidence: 99%

“…Long non-coding RNA XIST promotes chemoresistance of breast cancer cells to doxorubicin by sponging miR-200c-3p to upregulate the expression of ANLN, which is a downstream target gene of miR-200c-3p [25]. LncRNA-PNUTS serves as a competitive sponge for miR-205 during EMT, and its expression correlates with ZEB mRNA level [26].…”

Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA MPNCR is a Negative Regulator of Bovine Mammary Epithelial Cell Proliferation

Wang¹,

Zhang²,

Liu³

et al. 2020

Preprint

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BackgroundNumerous studies have reported that many long non-coding RNAs (lncRNAs) may play multiple essential roles during mammary gland development; however, little is known about their functions and regulatory mechanism.ResultsIn this study, we confirmed a novel lncRNA, MPNCR, that was highly expressed during lactation compared to the dry period in dairy cattle. The potential biological and regulatory functions of MPNCR were explored in bovine mammary epithelial cells (BMECs). The MTT and EdU assays revealed that lncRNA MPNCR significantly suppressed cell viability and proliferation of BMECs. The subcellular localization indicated that MPNCR had a potential function as a competing endogenous RNA (ceRNA) to regulate the proliferation of BMECs. Bioinformatics analysis showed that lncRNA MPNCR had two binding sites for miR-31, further confirmed by the dual-luciferase report assay. The RT-qPCR result showed that the expression of miR-31 was downregulated following the overexpression of MPNCR in BMECs. Furthermore, the expression of MPNCR was significantly inhibited by miR-31 overexpression and upregulated by miR-31 inhibition. Cell viability and proliferation were significantly suppressed or promoted with the transfection of miR-31 inhibitor or miR-31 mimic into BMECs, respectively, whereas rescue with MPNCR reduced the proliferation of BMECs. The dual-luciferase report assay showed an interaction between miR-31 and the 3' UTR of the target gene CAMK2D. RT-qPCR and western blot further showed that the expression of CAMK2D was significantly increased with the overexpression of miR-31, while its expression was significantly suppressed with the inhibition of miR-31, indicating CAMK2D was a target of miR-31.ConclusionTaken together, these results demonstrate the function and regulatory mechanism of lncRNA MPNCR on the proliferation of BMECs, and that its regulation may contribute to bovine mammary cell population management.

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“…In cervical cancer, bioinformatics analysis demonstrated that ANLN might be a candidate biomarker for evaluation of survival [7]. Meanwhile, ANLN awarded chemotherapy resistance ability to breast cancer and reduced the effectiveness of Doxorubicin or Anthracycline [8,9]. Based on large experiments, ANLN promotes the progression in cancer and might be a novel target in cancer therapy [10].…”

Section: Introductionmentioning

confidence: 99%

ANLN promotes carcinogenesis in oral cancer by regulating PI3K/mTOR signaling pathway

Wang

Zhang

et al. 2020

Preprint

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Background: Oral cancer is a malignant disease threatening human’s life and severely reduces human’s life-quality. Gene ANLN was reported to promote progression in cancer. This study aims at investigating the role of ANLN and molecular mechanism in oral cancer. Methods: ANLN was down-regulated by RNAi technology. The effect of ANLN on cell behaviors including proliferation, cycle distribution, invasion, and apoptosis was detected. Western blotting analysis was used to disclose the mechanism of ANLN in oral cancer. Results: ANLN was shown to express significantly higher in tumor tissues compared to the normal control tissues based on TCGA data. Patients with higher expression of ANLN displayed worse survival rate. Then ANLN was shown to express abundantly in cancer cell lines CA127 and HN30. When ANLN was reduced in CA127 and HN30 cells, cell proliferation and colony formation ability was inhibited. Cell invasion ability was suppressed. But cell apoptosis was induced reversely. In addition, expression of critical molecules including PI3K, mTOR, Akt, and PDK-1 were reduced after ANLN knockdown in CA127 cells. Conclusions: ANLN contributes to the progression in oral cancer and regulates activation of PI3K/mTOR signaling pathway. This study will provide guidance and new drug target for oral cancer.

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LncRNA XIST promotes chemoresistance of breast cancer cells to doxorubicin by sponging miR‐200c‐3p to upregulate ANLN

Cited by 48 publications

References 24 publications

Bioinformatics Analysis of the Regulatory lncRNA-miRNA-mRNA Network in Patients with Neuropathic Pain

Bioinformatics Analysis of the Regulatory lncRNA-miRNA-mRNA Network in Patients with Neuropathic Pain

Long Non-Coding RNA MPNCR is a Negative Regulator of Bovine Mammary Epithelial Cell Proliferation

ANLN promotes carcinogenesis in oral cancer by regulating PI3K/mTOR signaling pathway

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