Loading of DOX into a tetrahedral DNA nanostructure: the corner does matter

Abstract

With the rapid development of nanotechnology, various DNA nanostructures have been synthesized and widely used in the drug delivery. However, the underlying mechanisms of drug molecules loading into the DNA...

Recent progress of aptamer‒drug conjugates in cancer therapy

“…4 A). Since then, DOX has become the most popular model drug in drug delivery, with nucleic acid as a carrier 72 , 73 , 74 .

Figure 4 Examples of ApDC from physical conjugation.

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Direct visualization of the drug loading of single DNA origami nanostructures by AFM-IR nanospectroscopy

“…This can be achieved using different non-covalent binding modes, including intercalation, [12][13][14] groove-binding, [15][16][17] or simple electrostatic binding. [18][19][20] Depending on the drug molecule in question, these rather straightforward drug loading approaches unfortunately often suffer from complications such as a competition of several different binding modes, 15,21,22 limited accessibility of binding sites, 15,23 and difficulties in the unambiguous detection of drug-DNA binding events. The latter is usually attempted by analyzing binding-specific UV-vis or fluorescence spectroscopic signatures, which may, however, be obscured by or sometimes even originate in other processes such as drug aggregation.…”

Functionalized DNA tetrahedron for concomitant delivery of doxorubicin and paclitaxel

“…17 These drugloaded DNA nanostructures increase the e cacy of chemotherapy, decrease the adverse side effects and show no signi cant cytotoxicity in vivo and in vitro. 18,19 The DNA tetrahedron (TDN) is one of the most promising nanoconstructs due to its straightforward and predictable assembly in a quantitative manner. 19,20 Turber eld et al previously demonstrated the successful cellular uptake of TDN into mammalian cells 21 and Jiang et al described its' use as a DOX nanocarrier for targeted delivery into drug-resistant MCF7 cells.…”

“…18,19 The DNA tetrahedron (TDN) is one of the most promising nanoconstructs due to its straightforward and predictable assembly in a quantitative manner. 19,20 Turber eld et al previously demonstrated the successful cellular uptake of TDN into mammalian cells 21 and Jiang et al described its' use as a DOX nanocarrier for targeted delivery into drug-resistant MCF7 cells. 22 The uptake of TDN into mammalian cells is an energy-dependent endocytosis process.…”