Local Diversity and Biting Pattern of<i> Anopheles</i> Species in Southern Minahasa

Pinontoan, Odi R.; Supadmanaba, I Gede Putu; Manuaba, Ida Bagus Putra; Sukrama, I Dewa Made

doi:10.1155/2017/6313016

Cited by 6 publications

(4 citation statements)

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“…kochi has a habit of biting humans and domestic animals. Peak biting of humans and cattle occurs in the first half of the night [ 94 ], with a preference for one or the other depending on the distance to the blood source and its protected conditions. When livestock pens are distributed around human houses, the probability of mosquitoes feeding on human blood is elevated, so the probability of Plasmodium sporozoite infection increases [ 94 ].…”

Section: Discussionmentioning

confidence: 99%

“…Peak biting of humans and cattle occurs in the first half of the night [ 94 ], with a preference for one or the other depending on the distance to the blood source and its protected conditions. When livestock pens are distributed around human houses, the probability of mosquitoes feeding on human blood is elevated, so the probability of Plasmodium sporozoite infection increases [ 94 ]. Anopheles kochi plays a significant role in P. vivax and P. falciparum malaria transmission, which has been observed on the Bangladeshi–Indian border [ 95 – 98 ].…”

Section: Discussionmentioning

confidence: 99%

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Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border

et al. 2022

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Background To develop an effective malaria vector intervention method in forested international border regions within the Greater Mekong Subregion (GMS), more in-depth studies should be conducted on local Anopheles species composition and bionomic features. There is a paucity of comprehensive surveys of biodiversity integrating morphological and molecular species identification conducted within the border of Laos and Cambodia. Methods A total of 2394 adult mosquitoes were trapped in the Cambodia–Laos border region. We first performed morphological identification of Anopheles mosquitoes and subsequently performed molecular identification using 412 recombinant DNA–internal transcribed spacer 2 (rDNA-ITS2) and 391 mitochondrial DNA–cytochrome c oxidase subunit 2 (mtDNA-COII) sequences. The molecular and morphological identification results were compared, and phylogenetic analysis of rDNA-ITS2 and mtDNA-COII was conducted for the sequence divergence among species. Results Thirteen distinct species of Anopheles were molecularly identified in a 26,415 km2 border region in Siem Pang (Cambodia) and Pathoomphone (Laos). According to the comparisons of morphological and molecular identity, the interpretation of local species composition for dominant species in the Cambodia–Laos border (An. dirus, An. maculatus, An. philippinensis, An. kochi and An. sinensis) achieved the highest accuracy of morphological identification, from 98.37 to 100%. In contrast, the other species which were molecularly identified were less frequently identified correctly (0–58.3%) by morphological methods. The average rDNA-ITS2 and mtDNA-COII interspecific divergence was respectively 318 times and 15 times higher than their average intraspecific divergence. The barcoding gap ranged from 0.042 to 0.193 for rDNA-ITS2, and from 0.033 to 0.047 for mtDNA-COII. Conclusions The Cambodia–Laos border hosts a high diversity of Anopheles species. The morphological identification of Anopheles species provides higher accuracy for dominant species than for other species. Molecular methods combined with morphological analysis to determine species composition, population dynamics and bionomic characteristics can facilitate a better understanding of the factors driving malaria transmission and the effects of interventions, and can aid in achieving the goal of eliminating malaria. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border

et al. 2022

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“…barbirostris is an essential vector for the spread of malaria in the country. However, this species has never been reported in West Papua, but only in Kalimantan (Indriyati et al 2017), Sumatra (Yulidar 2017;Yahya et al 2020), Java (Ndoen et al 2010;Hastuti 2011) and Sulawesi (Pinontoan et al 2017).…”

Section: Discussionmentioning

confidence: 96%

Short Communication: The detection of Plasmodium in mosquitoes from Sumba and Sorong districts, Indonesia

et al. 2021

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Abstract. Munirah M, Wahid I, Hamid F, Wahyuni S. 2021. Short Communication: The detection of Plasmodium in mosquitoes from Sumba and Sorong districts, Indonesia. Biodiversitas 22: 2680-2684. Malaria is heading for elimination in 2030, but the disease remains prevalent in Indonesia. Therefore, this study aimed to determine a vector other than Anopheles that is potentially responsible for malaria transmission in the country's endemic areas. The mosquitoes were trapped by Kelambu trap, collected using a mouth aspirator, and stored in a tube containing silica gel. They were also examined microscopically following O'Connor identification keys, and Nested PCR was used to detect Plasmodium DNA. The number of mosquitoes collected from two areas was 1.336. In Sumba (Gaura village) district, 493 mosquitoes were captured, and the dominant genus was Anopheles (58.6%), followed by Culex (31%), Armigeres (9.9%), Aedes (0.2%), and Lutzia (0.2%). Among 843 mosquitoes collected from Sorong (Aimas), Culex was 98.9%, followed by Aedes (0.8%) and Anopheles (0.2%). The result of nested PCR found two An. sundaicus from Sumba carried Plasmodium which belongs to P. falciparum and P. vivax species (0.14%). Although we did not detect the presence of Plasmodium in mosquitoes other than the genus Anopheles, in this study, we found Anopheles species that have never previously been reported from the area, namely An. bailey and An. barbirostris from Papua and An. nivipes in West Sumba.

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“…vagus is found dominant in a location. (Alam et al, 2018;Astuti et al, 2016;Cooper, Edstein, Frances, & Beebe, 2010;Nguyen et al, 2021;Pinontoan, Supadmanaba, Manuaba, Sukrama, & Manuaba, 2017;Sugiarto, Hadi, Soviana, & Hakim, 2017).…”

Section: Anopheles Vagusmentioning

confidence: 99%

MOLECULAR DETECTION OF Plasmodium spp. IN Anopheles AND ITS VECTOR POTENTIAL IN LOW-ENDEMIC AREAS IN INDONESIA

Anggraeni¹,

Setiyaningsih²,

Mujiyanto³

et al. 2022

Proceeding of the International Conference on Public Health

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Indonesia has 31.1% of regencies/cities categorized as low-endemic areas. The areas are expected to accelerate the malaria elimination status; meanwhile, it is crucial to reduce the vector density in the area. Vector data updates in malaria-endemic areas are essential in anticipating the transmission and an increase in malaria cases. The purpose of the study was to get information on new malaria vector updates in low-endemic malaria areas in Indonesia. The study was conducted from 2015-2018 in Jambi, Riau Islands, Special Region of Yogyakarta, Central Kalimantan, North Kalimantan, and South Sulawesi. Mosquito and larva collection was performed in low-endemic areas of malaria. We used the night landing collection, animal-baited trap, livestock bait, light trap, and resting morning collection. The Plasmodium was identified from Anopheles samples using the polymerase chain reaction (PCR) with Plasmodium genus-specific primers. The results showed a new potential vector in low-endemic malaria areas in Indonesia. They are An. vagus (Jambi, Central Kalimantan, South Sulawesi, and Yogyakarta), An. kochi (Jambi), An. dirus and An. barbirostris (Central Kalimantan), and An. subpictus (South Sulawesi). The discovery of the suspected Anopheles species as malaria vectors suggests a potential transmission, and increased cases may occur. Vector surveillance needs to be strengthened to reduce malaria transmission.

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Local Diversity and Biting Pattern of Anopheles Species in Southern Minahasa

Cited by 6 publications

References 18 publications

Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border

Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border

Short Communication: The detection of Plasmodium in mosquitoes from Sumba and Sorong districts, Indonesia

MOLECULAR DETECTION OF Plasmodium spp. IN Anopheles AND ITS VECTOR POTENTIAL IN LOW-ENDEMIC AREAS IN INDONESIA

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