1981
DOI: 10.1073/pnas.78.9.5538
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Localization of 3' ends of 5S and 23S rRNAs in reconstituted subunits of Escherichia coli ribosomes.

Abstract: Periodate-oxidized 3' ends of 5S, 23S, and 16S rRNAs from Escherichia coli were allowed to react with fluorescein thiosemicarbazide, then labeled rRNAs were reconstituted into active ribosomal subunits. The fluorescein moiety on each of the rRNAs when reconstituted into ribosomal subunits was accessible to anti-fluorescein IgG as determined by fluorescence quenching and by sucrose gradient centrifugation. The region at which an antibody molecule bound to the labeled ribosomal subunits was determined by immunoe… Show more

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Cited by 49 publications
(21 citation statements)
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“…8A) is in precise agreement with the position found by neutron-scattering using E. coli proteins (May et al, 1992). Furthermore, this location is close to that determined for the 3'-terminus of 23s RNA (Shatsky et al, 1980;Stoffler-Meilicke et al, 1981), and RNA-protein cross-linking and footprinting studies have both identified sites for L3 in the vicinity of the extreme 3'-terminus of the 23s molecule (see Brimacombe, 1991, for review). In the model for the arrangement of the 50s proteins proposed by Walleczek et al (1988), L3 was placed on the basis of crosslinks to proteins L13 and L19, of which only L19 had been located by IEM; this placement of L3 was rather more towards the centre of the subunit than the position shown in Fig.…”
Section: Discussionsupporting
confidence: 71%
“…8A) is in precise agreement with the position found by neutron-scattering using E. coli proteins (May et al, 1992). Furthermore, this location is close to that determined for the 3'-terminus of 23s RNA (Shatsky et al, 1980;Stoffler-Meilicke et al, 1981), and RNA-protein cross-linking and footprinting studies have both identified sites for L3 in the vicinity of the extreme 3'-terminus of the 23s molecule (see Brimacombe, 1991, for review). In the model for the arrangement of the 50s proteins proposed by Walleczek et al (1988), L3 was placed on the basis of crosslinks to proteins L13 and L19, of which only L19 had been located by IEM; this placement of L3 was rather more towards the centre of the subunit than the position shown in Fig.…”
Section: Discussionsupporting
confidence: 71%
“…10). From this work a close neighbourhood of the two termini has emerged with the 5' terminus at a region at the lower left of the 30S/50S interface side (11) and the 3' terminus at the inner side of the large lobe (12,13,14).…”
Section: Introductionmentioning
confidence: 99%
“…The red and yellow colours are intended only to assist visualization, and follow the scheme of Capel et al (1988), as does the numerical labelling of the proteins. In the first column, the intersubunit interface of the small subunit is facing the viewer, which interpretation is supported by the presence of the interface protein S20/L26 (the two designations represent the same protein (Stöffler-Meilicke et al, 1981;Stöffler-Meilicke & Stöffler, 1990;Stade et al, 1995)). The bottom of the small ribosomal subunit is protein-deficient (Capel et al, 1988;Stöffler-Meilicke & Stöffler, 1990) and here is seen to be phosphorus-rich (i.e.…”
Section: Resultsmentioning
confidence: 73%
“…The independent reconstructions seem to have a natural fit when portrayed in this way. The merger was guided using published data on neutron scattering and immunolocalization of proteins and rRNA sequence segments (Stöffler & Wittmann, 1971;Stöffler-Meilicke et al, 1981;Stöffler & Stöffler-Meilicke, 1983;Capel et al, 1988;Walleczek et al, 1988;Stöffler-Meilicke & Stöffler, 1990;Nierhaus, 1991;May et al, 1992;Stade et al, 1995). If we interpret our E. coli ribosome reconstruction as a biologically functional entity, then there is a large central cavity which provides sufficient room to contain two or three molecules of transfer RNA (tRNA) and the necessary ancillary protein factors.…”
Section: Resultsmentioning
confidence: 99%