Localization of chemotactic activity and 64 kD protein phosphorylation for human polymorphonuclear leukocytes in N-terminus of the chemotactic protein LUCTIL-8

Suzuki, Kazuo; Koshio, Osamu; Ishida-Okawara, Akiko; Shibata, Michio; Yamakawa, Yoshio; Tagawa, Masatoshi; Ota, Hiromi; Kuramoto, Atsushi; Mizuno, Satoshi

doi:10.1016/0006-291x(89)91119-4

Cited by 20 publications

(7 citation statements)

References 11 publications

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“…fMet‐Leu‐Phe (fMLP) and superoxide dismutase (SOD) were obtained from Peptide Institute Inc., Osaka and ICN Immunobiologicals, Lisle, IL, respectively. IL‐8 was purified as previously described [8,9], and Millipore filter (type SS, pore size 3.0 μm) was obtained from Nihon Millipore Ltd., Yonezawa. 12‐ O ‐Tetradecanoylphorbol 13‐acetate (PMA) and Escherichia coli lipopolysaccharide (LPS) 055:B5 were purchased from Sigma Chemical Co. (St. Louis, MO) and Difco (Detroit, MI), respectively.…”

Section: Methodsmentioning

confidence: 99%

Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Kawai

Okawara

Okuyama

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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The ornithine-containing lipid (OL) and the serineglycine-containing lipid (SGL) of Flavobacterium activated and modulated the functions of human polymorphonuclear leukocytes (PMNs). The OL and the SGL strongly activated fMet-Leu-Phe- and interleukin-8-induced chemotaxis of PMNs at the concentration of 0.1 microg ml(-1), and a synthetic OL also activated the function of PMNs. Further, the OL strongly activated O(2)(-) production from PMNs. Although the OL and the SGL slightly modulated myeloperoxidase release from PMNs, inhibition effects of their component fatty acid analogues were observed. O(2)(-) production-inducing activity is a common biological activity between the OL and bacterial lipopolysaccharides, but OL and SGL, unlike lipopolysaccharide, are potent activators of PMN chemotaxis.

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Section: Methodsmentioning

confidence: 99%

Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Kawai

Okawara

Okuyama

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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“…fMet-Leu-Phe (fMLP) and superoxide dismutase (SOD) were obtained from Peptide Institute Inc., Osaka and ICN Immunobiologicals, Lisle, IL, respectively. IL-8 was puri-¢ed as previously described [8,9], and Millipore ¢lter (type SS, pore size 3.0 Wm) was obtained from Nihon Millipore 0928-8244 / 00 / $20.00 ß 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.…”

Section: Reagentsmentioning

confidence: 99%

Modulation of chemotaxis, O2− production and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid of Flavobacterium

Kawai¹

2000

FEMS Immunology and Medical Microbiology

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“…IL-8-1ike activity was determined by the migration distance of human neutrophils in a 3-gm-pored filter (Millipore, MA) using the modified Boyden chamber method as described elsewhere [17,18,20]. The crude sample was partially purified with cation-exchange chromatography using CL-6B Sepharose (Pharmacia, Uppsala, Sweden) equilibrated at pH 7.4, followed by recovery with 0.7 M NaC1 phosphate buffer.…”

Section: Assay For Neutrophil Chemomctic Activitymentioning

confidence: 99%

“…Furthermore, some human neoplastic cell lines, including lung giant cell carcinoma LU65C cells [17,18] and thyroid carcinoma KHM-5M cells [19], can produce IL-8. Recently, we demonstrated that human glioblastoma cells produce IL-8 in vitro and that IL-8 is detected in cerebrospinal fluid of patients bearing cerebral glioblastoma or bacterial meningitis [20,21].…”

Section: Introductionmentioning

confidence: 99%

Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877)

et al. 1993

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The production of interleukin 8 (IL-8), a neutrophil chemotactic factor, and its amino acid sequence were examined in glioblastoma cell lines in vitro. Neutrophil chemotactic activity was demonstrated in 9 conditioned media of 15 human glioblastoma cell lines. Tumor necrosis factor (TNF)-alpha stimulated secretion of the activity in 7 lines and induced secretion in 4 other lines. ELISA quantification disclosed that the conditioned media contained interleukin 8 (IL-8) in an amount equivalent to the chemotactic activity. The IL-8 secretion increased with the stimulation by TNF-alpha. Northern blot analysis and the RT-PCR method confirmed expression of mRNA in the glioblastoma cells and its augmentation by TNF-alpha and/or IL-beta. Reversed-phase HPLC following ion-exchange chromatography revealed that the chemotactic activity was a single peptide, which was determined to be IL-8 by the retention time and ELISA. Furthermore, amino acid analysis disclosed that a major part of the glioblastoma-cell derived IL-8 peptide was 77 amino acid IL-8 (IL-8(77); with the N-terminal sequence AVLPRSAKELRCQCI-).

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Localization of chemotactic activity and 64 kD protein phosphorylation for human polymorphonuclear leukocytes in N-terminus of the chemotactic protein LUCTIL-8

Cited by 20 publications

References 11 publications

Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Modulation of chemotaxis, O2− production and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid of Flavobacterium

Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877)

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Localization of chemotactic activity and 64 kD protein phosphorylation for human polymorphonuclear leukocytes in N-terminus of the chemotactic protein LUCTIL-8

Cited by 20 publications

References 11 publications

Modulation of chemotaxis, O2âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Modulation of chemotaxis, O2âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Modulation of chemotaxis, O2− production and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid of Flavobacterium

Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877)

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Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium

Modulation of chemotaxis, O₂^âproduction and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid ofFlavobacterium