Localization of chromogranin A and B, .BETA.-endorphin and enkephalins in the submandibular glands of mice.

Letic-Gavrilovic, Anka; Shibaike, Satoru; Niina, Masaaki; Naruse, Satoru; Abe, Kimio

doi:10.2330/joralbiosci1965.31.453

Cited by 3 publications

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“…A previous report stated that immunoreactivity of chromogranin A was present in GCT cells of mouse submandibular glands, and chromogranin B and C staining was absent in glandular cells (14,15 authors used anti-human chromogranin A monoclonal antibody (hybridoma LK2H10) specific for the human adrenal medulla (14,15). In the present study, we used rabbit antiserum agains to bovine SP-1 (chromogranin) (Incstar, Minnesota, USA) which recognized this antiserum and detected chromogranin A, B and C complex by immunoblotting test.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Letic-Gavrilovic et al (14,15) systemically identified immunodeposition for chromogranin A, B and C in mouse submandibular glands, and showed that chromogranin A staining is localized in granular convoluted tubule (GCT) cells, but chromogranin B and C staining is present in some solitary cells around the acini in mouse submandibular glands. They suggested that chromogranin A belongs to differentiating GCT …”

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Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Kunikata

Yamada

Ogata

et al. 1992

Acta Histochem. Cytochem

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The immunoreactivity of chromogranin was studied in postnatal salivary glands of the mice and rats. Chromogranin was observed in proacinar cells in both sexes during the postnatal developmental stage of the submandibular glands; the strongest reactivity was seen in 4th to 7th day specimens of mouse, and in 7th to 21st day specimens of rat. Acinar cells of mouse parotid glands showed positive staining in 1 to 42 days. No chromogranin staining was seen in granular convoluted tubule (GCT) cells of the submandibular glands in mice and rats. In sublingual glands of rat, reactivity of chromogranin was found in undifferentiated serous acinar cells, whereas in mice, it was absent in these cells. No sex difference of chromogranin staining was observed in submandibular glands in mice and rats. Immunoblotting of chromogranin using polyclonal anti-bovine SP-1/chromogranin antiserum showed in submandibular glands and adrenal glands of the rat consisted of chromogranin A, B and C. The results of this study suggest that the existence of chromogranin in proacinar cells of salivary glands is probably associated with the developmental phenomenon during the postnatal development.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Kunikata

Yamada

Ogata

et al. 1992

Acta Histochem. Cytochem

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Chromogranin B-like immunoreactivity in the mouse submandibular salivary gland during postnatal development

1990

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Localization of Chromogranins, Non-neuron-specific Enolase, and Different Forms of Somatostatins in the Submandibular Salivary Glands of Mice

Letic-Gavrilovic

Abe

1990

J Dent Res

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The localizations of chromogranins A, B, and C, neuron-specific enolase (NSE, gamma gamma-type) and non-NSE (alpha alpha-type), and different forms of somatostatins were immunocytochemically identified. The localizations were compared with those of epidermal growth factor (EGF) and nerve growth factor (NGF) in the submandibular salivary glands (SMG) of male mice at five to six weeks of age, with use of a variety of antibodies and the peroxidase-antiperoxidase (PAP) and avidin-biotin complex (ABC) detection methods. In the SMG of male mice, the major chromogranin present was chromogranin A, whereas chromogranins B and C were not detected at these ages by either method. Chromogranin A-like immunoreactivity was located in the granular convoluted tubule (GCT) cells of the SMG, whereas non-NSE immunoreactivity was observed throughout the duct system and in some acinar-associated cells. NSE was not detected in any part of the SMG. The distribution of chromogranin A and somatostatins in the GCT cells was similar to that of EGF and NGF. Our results strongly suggest that chromogranin A and somatostatins, but not chromogranin B or C, may be useful as a means of differentiation of the cells in the duct system of the SMG responsible for the production of biologically-active factors.

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Localization of chromogranin A and B, .BETA.-endorphin and enkephalins in the submandibular glands of mice.

Cited by 3 publications

References 36 publications

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Chromogranin B-like immunoreactivity in the mouse submandibular salivary gland during postnatal development

Localization of Chromogranins, Non-neuron-specific Enolase, and Different Forms of Somatostatins in the Submandibular Salivary Glands of Mice

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