Localization of ubiquitin specific protease 26 at blood-testis barrier and near Sertoli cell-germ cell interface in mouse testes

Yan, Lin; Hsu, T.-H.; Yen, Pauline H.

doi:10.1111/j.1365-2605.2010.01130.x

Cited by 23 publications

(30 citation statements)

References 50 publications

(53 reference statements)

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“…21,22 Fully developed spermatozoa isolated from whole mouse testes showed intense USP26 signals on the dorsal surface of the head, covering most of the frontal portion of the head as previously reported 17 ( Figure 1a). The USP26 and PNA signals partially overlapped and both covered the apical hook.…”

Section: Resultsmentioning

confidence: 49%

“…Wholemount sperm specimens were prepared according to a published protocol, 11 and immuno-gold staining was performed as previously described. 17 Perforatorial triangular cross-sections were photographed regardless whether they contained gold-labels or not, and the distribution of the gold-labels on the images was then analyzed.…”

Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

“…18 Spermatozoa were isolated from different regions of mouse epididymis according to Olson et al 11 They were fixed with 2% paraformaldehyde in phosphate-buffered saline and spun onto glass slides using the Shandon Cytospin 4 Cytocentrifuge (Thermo Scientific, Pittsburgh, PA, USA). The cells were stained with the anti-USP26-M antibody 17 and an Alexa Fluor 488 conjugated goat anti-rabbit IgG secondary antibody (Invitrogen, Carlsbad, CA, USA) according to Lin and Yen. 19 The acrosome was labeled with lectin peanut agglutinin (PNA) conjugated with Alexa Fluor 594 (Invitrogen) or a mouse monoclonal anti-acrosin antibody (GeneTex, Inc., Irvine, CA, USA), and the nucleus was stained with Hoechst 33258 (Invitrogen).…”

Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

“…However, the presence of multiple bands on Western blots hinted possible crossreaction of the antibody with other proteins. 17 Here we report our subsequently immunostaining of mouse epididymal sperm with the antibody that led to the discovery of a novel structure we have named 'hook rim (HR)' at the three edges of the triangular rod of the anterior perforatorium. Examination of sperm from various regions of mouse epididymis revealed that sperm acquire HRs during epididymal maturation.…”

Section: Introductionmentioning

confidence: 99%

“…17 The antibody specifically stained cultured cells transfected with an USP26 expression vector but not the empty vector, and immunoprecipitated USP26 with deubiquitinating enzymatic activity from mouse testis lysates. However, the presence of multiple bands on Western blots hinted possible crossreaction of the antibody with other proteins.…”

Section: Introductionmentioning

confidence: 99%

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Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Lin¹,

Hsu²,

Yen³

2013

Asian J Androl

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Spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.

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Section: Resultsmentioning

confidence: 49%

Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Lin¹,

Hsu²,

Yen³

2013

Asian J Androl

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X Chromosome and Spermatogenesis Defects

Stouffs

Lissens

2013

Encyclopedia of Life Sciences

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Spermatogenesis, the process through which mature spermatozoa are formed, is a complex process requiring a very strict regulation. More than 2000 genes are involved in spermatogenesis. Part of these genes has a unique function during spermatogenesis. The X chromosome is special because only one copy is present in men, whereas women have two X copies. Moreover, the mammalian X chromosome is enriched for testis‐specific genes. This makes the X chromosome particularly interesting in view of male infertility. Although multiple studies have tried to identify mutations in X‐linked spermatogenesis genes, the number of causative mutations detected so far remains low. Nowadays, new techniques allow the analysis of the complete X chromosome in a single experiment. Within the next few years, the knowledge on the role of the X chromosome in male infertility (and consequently also in spermatogenesis) will increase. Key Concepts: Men are hemizygous for X‐chromosomal genes: men only have one X chromosome; mutations in X‐linked genes (might) have an immediate impact on spermatogenesis. Spermatogenic failure can be caused by genetic defects. The X chromosome is enriched for spermatogenesis genes. Many multi‐copy genes involved in spermatogenesis are also expressed in cancer cell lines. Therefore, these are often described as cancer‐testis (CT) genes. Many X‐linked genes that are preferentially expressed in testis have not (yet) been evaluated in view of male infertility. The study of individual genes in view of male infertility has been disappointing because it is a time‐consuming process and often no differences with normal controls have been detected.

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Concise Review: Fate Determination of Stem Cells by Deubiquitinating Enzymes

et al. 2016

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Post-translational modification by ubiquitin molecules is a key regulatory process for stem cell fate determination. Ubiquitination and deubiquitination are the major cellular processes used to balance the protein turnover of several transcription factors that regulate stem cell differentiation. Deubiquitinating enzymes (DUBs), which facilitate the processing of ubiquitin, significantly influence stem cell fate choices. Specifically, DUBs play a critical regulatory role during development by directing the production of new specialized cells. This review focuses on the regulatory role of DUBs in various cellular processes, including stem cell pluripotency and differentiation, adult stem cell signaling, cellular reprogramming, spermatogenesis, and oogenesis. Specifically, the identification of interactions of DUBs with core transcription factors has provided new insight into the role of DUBs in regulating stem cell fate determination. Thus, DUBs have emerged as key pharmacologic targets in the search to develop highly specific agents to treat various illnesses. STEM CELLS 2017;35:9-16 SIGNIFICANCE STATEMENTThe review is written from a unique perspective in overseeing the regulatory role of deubiquitinating enzymes in stem cells. A great attention has been given to the physiological role of ubiquitination system in regulating pluripotency of embryonic stem cells. However, the reversal of ubiquitination by deubiquitinating enzymes plays an equally important role in the regulation of levels of expression of the stemness-related proteins by preventing its ubiquitination. This is timely topic, given that this is the first review article attempting to discuss the accumulated evidence suggesting the critical role of deubiquitinating enzymes in regulating stem cell fate determination process such as maintenance of pluripotency, differentiation, cellular reprogramming, spermatogenesis, and oogenesis. Beyond this, the review provide us the clue for the remaining challenges in developing DUBs as targets for stem cell therapeutics.

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Localization of ubiquitin specific protease 26 at blood-testis barrier and near Sertoli cell-germ cell interface in mouse testes

Cited by 23 publications

References 50 publications

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

X Chromosome and Spermatogenesis Defects

Concise Review: Fate Determination of Stem Cells by Deubiquitinating Enzymes

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