Loktanella variabilis sp. nov., isolated from a tidal flat

Abstract

Two Gram-negative, aerobic and rod-shaped or ovoid bacterial strains with different colony colours (greyish yellow and moderate orange), designated J-MR2-Y T and J-MR2-O, were isolated from a tidal flat in the South Sea of South Korea. The two novel strains grew optimally at 35-37 6C. Strains J-MR2-Y T and J-MR2-O showed no difference in their 16S rRNA gene sequences, and the mean DNA-DNA relatedness between them was 94 %. Phylogenetic trees based on 16S rRNA gene sequences revealed that strains J-MR2-Y T and J-MR2-O clustered consistently with the type strains of Loktanella soesokkakensis, L. hongkongensis and L. cinnabarina, with which it exhibited 97.83-99.06 % sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus Loktanella were 94.01-96.26 %. Both strains contained Q-10 as the predominant ubiquinone, C 18 : 1 v7c as the major fatty acid and phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid as the major polar lipids. The DNA G+C contents of strains J-MR2-Y T and J-MR2-O were 68.1 and 68.4 mol%, respectively, and DNA-DNA relatedness values with the type strains of L. soesokkakensis, L. hongkongensis and L. cinnabarina were 19-37 %. Differential phenotypic properties, together with their phylogenetic and genetic distinctiveness, revealed that the two novel strains are separated from other species of the genus Loktanella. On the basis of the data presented, strains J-MR2-Y T and J-MR2-O are proposed to represent a novel species of the genus Loktanella, for which the name Loktanella variabilis sp. nov. is proposed. The type strain is J-MR2-Y T (5KCTC 42074 T 5CECT 8572 T ).

Pseudoroseicyclus aestuarii gen. nov., sp. nov., isolated from a tidal flat

“…The polar lipid profile of strain DB-4 T was also distinguishable from those of the type strains of some phylogenetically related Loktanella species by the absence of one unidentified glycolipid ( Fig. S2; S. Park et al, 2013Park et al, , 2014aJ. M. Park et al, 2014).…”

“…The differences in fatty acid and polar lipid profiles served to differentiate strain T from the type strains of R. mahoneyensis and some phylogenetically related species of the genus Loktanella (Table 1; Fig. S2; S. Park et al, 2013Park et al, , 2014a; J. M. Park et al, 2014). Strain DB-4 T was distinguished from the type strain of R. mahoneyensis by differences in several phenotypic characteristics, including utilization of and acid production from some substrates, activity of some enzymes, susceptibility to some antibiotics and DNA G+C contents (Table 2).…”

“…The type species is . Data for columns 1-4 are from this study; data for columns 5 and 6 are from S. Park et al (2013); data for column 7 are from J. M. Park et al (2014). Fatty acids that represented < 0.5 % in all columns were omitted.…”

Limimaricola litoreus sp. nov., isolated from intertidal sand of the Yellow Sea

“…The major cellular fatty acids (>10 %) of strain ASW11-118 T was C 18 : 1 ω 7 c (84.2%), with C 16 : 0 (5.3%), summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c , 2.9%), C 18 : 0 (2.0%), C 12 : 0 3-OH (2.0%) and C 10 : 0 3-OH (1.1%) as minor components (Table S4). The predominance of C 18 : 1 ω 7 c is prevalent in the genus Limimaricola [1–7]. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), one unknown aminolipid (AL), one unknown phospholipid (PL) and two unidentified lipids (L1 and L2) (Fig.…”

Loktanella marina sp. nov., isolated from seawater

“…Subsequently, the names Loktanella hongkongensis (Lau et al, 2004), Loktanella agnita and Loktanella rosea (Ivanova et al, 2005), Loktanella koreensis (Weon et al, 2006), Loktanella atrilutea (Hosoya & Yokota, 2007), Loktanella maricola (Yoon et al, 2007), Loktanella pyoseonensis (Moon et al, 2010), Loktanella tamlensis (Lee, 2012), Loktanella cinnabarina (Tsubouchi et al, 2013), Loktanella litorea , Loktanella sediminilitoris (Park et al, 2013), Loktanella maritima (Tanaka et al, 2014), Loktanella ponticola (Jung et al, 2014), Loktanella soesokkakensis (Park et al, 2014a), Loktanella variabilis (Park et al, 2014b) and Loktanella sediminum (Liang et al, 2015) have been validly published. In this study, we describe a bacterial strain, designated MDM-7 T , which was isolated from seawater collected at Taean of the Yellow Sea in South Korea.…”