Long‐living nanobubbles of dissolved gas in aqueous solutions of salts and erythrocyte suspensions

Abstract

Results of experiments combining laser modulation interference microscopy and Mueller matrix scatterometry show that macroscopic scatterers of light are present in liquids free of external solid impurities. Experimental data on distilled water and aqueous NaCl solutions of various concentrations as well as physiological saline solution are reported. The experimental data can be interpreted by using a model of micron-scale clusters composed of polydisperse air nanobubbles having effective radii of 70-100 nm. Their concentration increases with the growth of ionic content. We hypothesize that under certain conditions those clusters of nanobubbles can affect the erythrocyte structure.

Structure of the nanobubble clusters of dissolved air in liquid media

“…Assuming that at breathing the fish captures the bubston clusters by gills, the certain problem associated with the extraction of molecules of dissolved oxygen disappears. However, as was shown in our recent experimental work [18], for the bubstons and bubston clusters to exist in water the increased content of ions is necessary. Besides, in the same work it was shown that the bubston clusters play a negative role in living tissues at a sharp decrease of arterial pressure.…”

“…Refs. [14][15][16][17][18][19][20] contain a direct experimental proof of the bubston existence in an equilibrium aqueous solution of salts, saturated by dissolved air. However, key evidence supporting this hypothesis was inferred from observations of low-threshold laser-induced breakdown in water and aqueous electrolytic solutions that were transparent to the laser beams employed [21][22][23][24][25][26].…”

Laser Interference Microscopy for Nanobiotechnologies

“…Therefore, in this case the erythrocytes contain bubstone clusters [14]. In normal erythrocytes the maximum of μ distribu tion is around 0.3.…”

Untitled

“…This technique appeared to be very effective for the study of colloid systems; see our previous works (Bunkin et al 2009a;Bunkin et al 2009b;Bunkin et al 2010;Bunkin et al 2011;Bunkin et al 2012a;Bunkin et al 2012b). The microscope is a two-channel device.…”