Long non-coding RNA-2271 promotes osteogenic differentiation in human bone marrow stem cells

Xi, Li-Cheng; Li, Hongyu; Yin, Dong

doi:10.1515/biol-2018-0049

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Also, the histological model of formation of HLs could be improved-there is no unanimity whether the HLs are rather Bgrowth arrest^ (Scott and Hoppa 2015) or Bgrowth recovery lines^ (Sajko et al 2011). Current biochemical models assume the involvement of GH and IGF-1 (Alfonso-Durruty 2011); however, bone growth and mineralization may be connected also with other proteins and pathways, such as TAZ/Runx2 and PI3K/Akt (Alkagiet and Tziomalos 2017;Zhang et al 2017), or could involve miRNA (Han et al 2017) and long non-coding RNA (Xi et al 2018). Better understanding of the biochemical base of HL formation could improve the interpretation of results involving these lines.…”

Section: Summary Perspectives and Challengesmentioning

confidence: 99%

How to calculate the age at formation of Harris lines? A step-by-step review of current methods and a proposal for modifications to Byers’ formulas

Kulus

Dąbrowski

2019

Archaeol Anthropol Sci

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Harris lines (HL; also known as Bgrowth arrest lines^or Btransverse radiopaque lines^) are horizontal sclerotic lines formed in the metaphyseal or diaphyseal part of long bones, usually visualized using X-ray images. Among the factors that may lead to a temporary arrest of bone growth (and thus-to HL deposition), the most commonly mentioned are nutritional disorders (malnutrition, protein, vitamin, and mineral deficiencies), a history of smallpox, pneumonia or other diseases, food poisoning, or alcohol abuse. The position of the HL is related to the period of incidence of physiological stress inhibiting bone growth, which enables the estimation of the age at which the subject was exposed to it. Such information can be valuable in the study on archeological populations; therefore, various methods have been developed to determine the age of HL deposition. In this review, six known methods for calculating the age of HL origin are presented and compared: Allison/McHenry, Hunt and Hatch, Clarke, Hummert and van Gerven, and Maat and Byers' methods. In addition, the authors propose here a modification to the last method in order to enable calculations on non-adult bones.

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Section: Summary Perspectives and Challengesmentioning

confidence: 99%

How to calculate the age at formation of Harris lines? A step-by-step review of current methods and a proposal for modifications to Byers’ formulas

Kulus

Dąbrowski

2019

Archaeol Anthropol Sci

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“…Bone marrow mesenchymal stem cells are ideal seed cells; however, there are many hematopoietic stem cells in bone marrow. Therefore, it is difficult to separate mesenchymal stem cells from bone marrow due to contamination of hematopoietic stem cells [ 7 ]. Because there are also mesenchymal stem cells in the bone cortex, but there are basically no hematopoietic stem cells, the separation of mesenchymal stem cells from the bone cortex can avoid hematopoietic stem cell contamination [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 µm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells

Yang,

Qiu,

Zhou

et al. 2023

Ann Transplant

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Background Many patients have bone defects that exceed the healing size. This study aimed to construct polycaprolactone/nano-hydroxyapatite (PCL/nHA) composite scaffolds with different pore sizes and investigate the osteogenesis and histocompatibility of cortical bone mesenchymal stem cells (BMSCs-C) seeded on it after inoculation. Material/Methods After mixing PCL and nHA proportionally, three-dimensional (3D) printing was used to print scaffolds. Porosity, compressive strength, and elastic modulus of PCL/nHA scaffolds were tested. The proliferation of BMSCs-C cells was examined and osteogenesis, chondrogenesis, and adipogenesis were evaluated. BMSCs-C cells were inoculated into 3D printing scaffolds, and histocompatibility between BMSCs-C cells and scaffolds was observed by the cell count kit (CCK-8) assay and LIVE/DEAD staining. After inoculating BMSCs-C cells into scaffolds, alkaline phosphatase (ALP) activity and calcium content were measured. Results There was no obvious difference in characteristics between the 3 PCL/nHA composite scaffolds. The porosity, compressive strength, and elastic modulus of the 300/500-μm scaffold were between those of the 300-μm and 500-μm scaffolds. With increasing pore size, the mechanical properties of the scaffold decrease. BMSCs-C cells demonstrated faster growth and better osteogenic, adipogenic, and chondrogenic differentiation; therefore, BMSCs-C cells were selected as seed cells. PCL/nHA composite scaffolds with different pore sizes had no obvious toxicity and demonstrated good biocompatibility. All scaffolds showed higher ALP activity and calcium content. Conclusions The 300/500 μm mixed pore size scaffold took into account the mechanical properties of the 300 μm scaffold and the cell culture area of the 500 μm scaffold, therefore, 300/500 μm scaffold is a better model for the construction of tissue engineering scaffolds.

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The effects of SDF-1 combined application with VEGF on femoral distraction osteogenesis in rats

Fu,

Li,

Yang

et al. 2024

Open Life Sciences

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Bone regeneration and mineralization can be achieved by means of distraction osteogenesis (DO). In the present study, we investigated the effect of stromal cell-derived factor 1 (SDF-1) and vascular endothelial growth factor (VEGF) on the new bone formation during DO in rats. Forty-eight Sprague–Dawley rats were randomized into four groups of 12 rats each. We established the left femoral DO model in rats and performed a mid-femoral osteotomy, which was fixed with an external fixator. DO was performed at 0.25 mm/12 h after an incubation period of 5 days. Distraction was continued for 10 days, resulting in a total of 5 mm of lengthening. After distraction, the solution was locally injected into the osteotomy site, once a day 1 ml for 1 week. One group received the solvent alone and served as the control, and the other three groups were treated with SDF-1, VEGF, and SDF-1with VEGF in an aqueous. Sequential X-ray radiographs were taken two weekly. The regeneration was monitored with the use of micro-CT analysis, mechanical testing, and histology. Radiographs showed accelerated regenerate ossification in the SDF-1, VEGF, and SDF-1 with the VEGF group, with a larger amount of new bone compared with the control group, especially SDF-1 with the VEGF group. Micro-CT analysis and biomechanical tests showed Continuous injection of the SDF-1, VEGF, and SDF-1 with VEGF during the consolidation period significantly increased bone mineral density bone volume, mechanical maximum loading, and bone mineralization of the regenerate. Similarly, the expression of osteogenic-specific genes, as determined by real-time polymerase chain reaction , was significantly higher in SDF-1 with the VEGF group than in the other groups. Histological examination revealed more new trabeculae in the distraction gap and more mature bone tissue for the SDF-1 with the VEGF group. SDF-1 and VEGF promote bone regeneration and mineralization during DO, and there is a synergistic effect between the SDF-1 and VEGF. It is possible to provide a new and feasible method to shorten the period of treatment of DO.

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Long non-coding RNA-2271 promotes osteogenic differentiation in human bone marrow stem cells

Cited by 3 publications

References 26 publications

How to calculate the age at formation of Harris lines? A step-by-step review of current methods and a proposal for modifications to Byers’ formulas

How to calculate the age at formation of Harris lines? A step-by-step review of current methods and a proposal for modifications to Byers’ formulas

Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 µm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells

The effects of SDF-1 combined application with VEGF on femoral distraction osteogenesis in rats

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