Long Noncoding RNA Small Nucleolar RNA Host Gene 1 (SNHG1) Promotes Renal Cell Carcinoma Progression and Metastasis by Negatively Regulating miR-137

Abstract

BackgroundData on the expression of RCC tissues from the GEO database and patient survival data from TCGA were used to explore the prognostic significance of long noncoding RNA SNHG1. SNHG1 has been reported to participate in the development of several cancers, but, the underlying mechanism of SNHG1 in renal cell carcinoma (RCC) has not been reported. The purpose of our study was to investigate the potential function of SNHG1 in RCC.Material/MethodsThe expression of SNHG1 in 40 cases of RCC and adjacent normal tissues and 5 cell lines was detected by qRT-PCR. Cell proliferation, Transwell assay, and Western blotting assay were carried out to investigate the biological function of SNHG1. A rescue experiment was performed to verify that miR-137 can partly impede the effect of SNHG1 on renal cancer cells.ResultsSNHG1 was identified to be overexpressed in RCC tissues and RCC cell lines. High levels of SNHG1 were correlated with poor prognosis of RCC patients. Knockdown of SNHG1 suppressed the proliferation, invasion, and EMT capacity in RCC. Moreover, miR-137 abrogated the effect of SNHG1 on RCC.ConclusionsSNHG1 is significantly upregulated in RCC and renal cancer cell lines. Overexpression of SNHG1 participates in RCC tumorigenesis by regulating miR-137.

Small nucleolar RNA host gene 1 promotes development and progression of colorectal cancer through negative regulation of miR‐137

“…miR‐137 is located on human chromosome 1p22 and is involved in many biological processes and diseases . To date, research indicates that miR‐137 may play a dual role during tumorigenesis, and that the nature of this role may be dependent on tumor type and target messenger RNA (mRNA) identities . As a tumor suppressor in melanoma, miR‐137 may inhibit cell migration by targeting the TBX3 transcription factor .…”

“…indicates that miR-137 may play a dual role during tumorigenesis, and that the nature of this role may be dependent on tumor type and target messenger RNA (mRNA) identities. 30,31 As a tumor suppressor in melanoma, miR-137 may inhibit cell migration by targeting the TBX3 transcription factor. 32 As an oncogene in breast cancer, miR-137 may enhance the epithelial-to-mesenchymal transition (EMT) | 2113 capacity of tumor cells by inhibiting the function of BMP7.…”

<p>miR-106b promotes proliferation and invasion by targeting Capicua through MAPK signaling in renal carcinoma cancer</p>

“…Since epithelial-mesenchymal transition (EMT) plays a crucial role in the metastasis of various cancer cells including renal carcinoma cancer, 28,29 the expressions of EMT-related protein were examined in 786-O cells transfected with target microRNAs. The results demonstrated miR-106b silencing in 786-O cells markedly downregulated the expression of N-cadherin and vimentin, while upregulated the expression of E-cadherin, which lead to the inhibition of EMT (Figure 4).…”

Identification of the Potential Prognostic Markers from the miRNA-lncRNA-mRNA Interactions for Metastatic Renal Cancer via Next-Generation Sequencing and Bioinformatics

“…The molecular function of some miRNAs in RCC has been determined in previous studies. The current studies suggest that has-miR-137 may serve as a suppressor of tumor progression and metastasis in RCC [54,55]. Besides, the tumor suppressor function of hsa-miR-224-5p and hsa-miR-335-5p is also demonstrated in clear cell RCC [57,58].…”

“…Before the present study, various studies demonstrated that miRNAs such as hsa-miR-137 [54,55], hsa-mir-211-5p [56], hsa-mir-224-5p [57], hsa-miR-335-5p [58], and hsa-mir-296-3p [59], are involved in the regulation of RCC progression. In addition, increased hsa-miRNA-9 and decreased hsa-miRNA-200a could serve as biomarkers for distinguishing hemangioblastomas from metastatic clear cell RCC [60].…”